A5039031303- Genomics and Chromatin Dynamics
- BRCA gene mutations in cancer
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- interferon and immune responses
- Protein Degradation and Inhibitors
- Regional Socio-Economic Development Trends
- Cancer-related gene regulation
- Nutrition, Genetics, and Disease
- CRISPR and Genetic Engineering
- Regional Economic Development and Innovation
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- NF-κB Signaling Pathways
- Socioeconomic and Demographic Analysis
- Microbial Community Ecology and Physiology
- Global Health Care Issues
- Migration, Aging, and Tourism Studies
- Toxin Mechanisms and Immunotoxins
- Economic and Technological Developments in Russia
- Chemical Reactions and Isotopes
- Estrogen and related hormone effects
Institute of Economics
2022-2024
Institute of Ecology and Genetics of Microorganisms
2017-2023
Ministry of Health of the Russian Federation
2015-2022
Université de Montréal
2017
Russian Cancer Research Center NN Blokhin
2015
Hôpital Maisonneuve-Rosemont
2013-2014
Université Paris Cité
1998-2012
Délégation Paris 5
2006-2012
Dana-Farber Cancer Institute
2006-2011
Harvard University
2006-2011
In response to DNA double-strand breaks (DSBs), BRCA1 forms biochemically distinct complexes with certain other damage proteins. These structures, some of which are required for homologous recombination (HR)-type DSB repair, concentrate at nuclear foci that demarcate sites genome breakage. Polyubiquitin binding by one these the RAP80/BRCA1 complex, is efficient focal recruitment, but relationship this process execution HR has been unclear. We found complex actively suppresses otherwise...
beta-actin mRNA is localized near the leading edge in several cell types, where actin polymerization actively promoting forward protrusion. The localization of facilitated by a short sequence 3' untranslated region, "zip code." Localization at this region important physiologically. Treatment chicken embryo fibroblasts with antisense oligonucleotides complementary to (zip code) leads delocalization mRNA, alteration phenotype, and decrease motility. To determine components process responsible...
The microtubule-associated tau protein participates in the organization and integrity of neuronal cytoskeleton. A nuclear form has been described non-neuronal cells, which displays a nucleolar localization during interphase but is associated with nucleolar-organizing regions mitotic cells. In present study, based on immunofluorescence, immuno-FISH confocal microscopy, we show that mainly at internal periphery nucleoli, partially colocalizing nucleolin human AT-rich α-satellite DNA sequences...
Induction of the antiviral interferon response is initiated upon recognition viral RNA structures by RIG-I or Mda-5 DEX(D/H) helicases. A complex signaling cascade then converges at mitochondrial adapter MAVS, culminating in activation IRF and NF-kappaB transcription factors induction gene expression. We have previously shown that MAVS recruits IkappaB kinase epsilon (IKKepsilon) but not TBK-1 to mitochondria following infection. Here we map interaction IKKepsilon C-terminal region...
ABSTRACT The induction of the beta interferon (IFN-β) gene constitutes one first responses cell to virus infection. Its regulation is achieved through an intricate combination virus-induced binding transcription factors and local chromatin remodeling. In this work, we demonstrate that factor YY1, known interact with histone deacetylases (HDAC) acetyltransferases, has a dual activator/repressor role during IFN-β promoter activity. We show YY1 specifically binds in vitro vivo murine at...
ABSTRACT The induction of alpha/beta interferon (IFN-α/β) genes constitutes one the first responses cell to virus infection. IFN-β gene is constitutively repressed in uninfected cells and transiently activated after In this work we demonstrate that histone deacetylation regulates silent state murine gene. Using chromatin immunoprecipitation (ChIP) assays, show a direct vivo correlation between transcriptionally hypoacetylation H4 on promoter region. Trichostatin A (TSA), specific inhibitor...
Pericentromeric γ-satellite DNA is organized in constitutive heterochromatin structures. It comprises a 234 bp sequence repeated several thousands times surrounding the centromeric of all murine chromosomes. Potential binding sites for transcription factor Yin Yang 1 (YY1), repressor or activator cellular and viral genes, are present pericentromeric DNA. Using gel retardation chromatin immunoprecipitation, we demonstrate this work that YY1 specifically interacts vitro vivo with immunoFISH...
The addition of 5 mM cAMP to a cell‐free translation system from rabbit reticulocytes increases the rate protein synthesis 3–5‐fold. Lower concentrations (0.005, 0.05 and 0.5 mM) have no effect on in this system. at all tested stimulates phosphorylation same pattern polypeptides, while additionally dephosphorylation 95 kDa polypeptide identified as elongation factor 2 (EF‐2). Testing preparations EF‐2 with different content phosphorylated form poly(U)‐directed poly(Phe) reveals that activity...
The distribution of eukaryotic elongation factor 2 (eEF-2) in G0-arrested fixed human skin diploid fibroblasts was studied by indirect immunofluorescent microscopy. It found earlier that the main part eEF-2 cycling cells located near nucleus endoplasm (Gavrilova et al., 1987). has been demonstrated here transition from proliferation to G0 phase cell cycle leads mainly along intermediate filaments and/or microtubules. Both and a portion is also co-localized with actin microfilament bundles....
Indirect immunofluorescent microscopy was used to study the distribution of elongation factor 2 (eEF-2) in fixed human skin diploid and mouse embryo fibroblasts. It found earlier that some eEF-2, ribosomes initiation (eIF-2) are co-localized with a part actin microfilament bundles these cells (Gavrilova et al., 1987; Shestakova 1991). Here it has been shown inhibition protein synthesis either by inactivation eEF-2 itself diphtheria toxin or ricin does not abolish along bundles. At same time,...
The fusion protein E2A-PBX1 induces pediatric B cell leukemia in human. Previously, we reported oncogenic interactions between homeobox (Hox) genes and murine T leukemia. A proviral insertional mutagenesis screen with our mouse model identified Hoxa as potential collaborators to E2A-PBX1. Here studied whether Hoxa9 could enhance leukemogenesis.We show that confers a proliferative advantage cells. Transplantation experiments transgenic cells overexpressing isolated from bone marrow chimeras...
Nuclear architecture as well gene nuclear positioning can modulate expression. In this work, we have analyzed the position of interferon-β (IFN-β) locus, responsible for establishment innate antiviral response, with respect to pericentromeric heterochromatin (PCH) in correlation virus-induced IFN-β Experiments were carried out two different cell types either non-infected (NI) or during time course three viral infections. NI cells, showed a monoallelic promoter association PCH that strongly...
In our days it becomes clear that not only the genetic information encoded in sequence of DNA is responsible for gene expression profile but epigenetic signature pattern modifications histones associate with chromosomes also plays a very important role determination expression.Octomere (H3/H4 and H2A/H2B) segment surrounding histone proteins compose nucleosome, basic unit chromatin turn first level chromosome organization.Besides hypermethylation CpG islands leads to repression multiple...
The tumor suppressor breast cancer 1, early onset (BRCA1) plays an important role in maintaining the genome integrity, actively participating DNA repair via homologous recombination. level of functional final BRCA1 protein is precisely regulated cells. Epigenetic regulation gene expression one most mechanisms for fine-tuning. In this review main epigenetic are presented, including methylation, histone covalent modifications, and with transcription factors. Another function emerging recent...