A5030755987- Immune cells in cancer
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Dermatology and Skin Diseases
- Angiogenesis and VEGF in Cancer
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Retinoids in leukemia and cellular processes
- Cerebrospinal fluid and hydrocephalus
- Gut microbiota and health
- Inflammasome and immune disorders
- Adenosine and Purinergic Signaling
- Clostridium difficile and Clostridium perfringens research
- Fetal and Pediatric Neurological Disorders
- Neonatal Respiratory Health Research
- Head and Neck Surgical Oncology
- Lymphatic System and Diseases
- Hematopoietic Stem Cell Transplantation
- Blood disorders and treatments
- Cancer Cells and Metastasis
University of British Columbia
2021-2025
University of Calgary
2018-2020
Significance Staphylococcus aureus is a bacterium causing many community-acquired skin infections. However, as dissemination from infections rare, we were interested in understanding what mechanisms the body has place to prevent escape of bacteria via lymphatics rest body. We found that after infection, neutrophils almost immediately recruited lymph nodes specialized blood vessels cut off at pass. Neutrophils worked together with resident macrophages S. spread, removing both these immune...
Abstract Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and cell recruitment in lymphoid tissues. However, diversity of BEC their origins during angiogenesis remain unclear. Here we profile transcriptomes from peripheral lymph nodes map phenotypes to vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts selective role myeloid (vs lymphocyte) medulla, confirmed videomicroscopy. define five...
Essentially all Staphylococcus aureus (S. aureus) bacteria that gain access to the circulation are plucked out of bloodstream by intravascular macrophages liver - Kupffer cells. It is also thought these disseminated via other organs. Our data show S. inside cells grew and escaped across mesothelium into peritoneal cavity immediately infected GATA-binding factor 6-positive (GATA6+) macrophages. These provided a haven for aureus, thereby delaying neutrophilic response in peritoneum 48 hours...
The adaptive immune function of lymph nodes is dependent on constant recirculation lymphocytes. In this article, we identify neutrophils present in the node at steady state, exhibiting same capacity for recirculation. germ-free mice, still recirculate through nodes, and mice cohoused with wild microbiome level increases significantly. We found that enter entirely via L-selectin actively exit efferent lymphatics an S1P mechanism. small population can act as reconnaissance cells to recruit...
Organoid-based research has made significant discoveries and contributions to our understanding of human organ function in both health disease. To continue making progress, it is crucial acknowledge the role immune system all organs. Various cells, such as macrophages, T neutrophils, are resident almost tissues play essential roles homeostasis, function, Using diverse methods, researchers have begun integrating cells into organoid models, leading more physiologically relevant models that...
Lymphoid organs are critical for organizing the development of immune system, generating tolerance, and orchestrating adaptive response to foreign antigens. Defects in their structure function can lead immunodeficiency, hypersensitivity, cancer, or autoimmune diseases. To better understand these diseases assess potential therapies, complex models that recapitulate anatomy physiology tissues required. Organoid possess a number advantages, including 3D microarchitecture, scalability,...
Abstract Upon tissue injury or microbial invasion, a large number of neutrophils converge from blood to the sites infection in short time. The migration through limited paths tissues and capillary networks seems efficient ‘traffic jams’ are generally avoided. However, mechanisms that guide trafficking numbers not well understood. Here we show pairs arriving closely one after another at bifurcations migrate alternating branches vivo vitro. Perturbation chemoattractant gradients increased...
Abstract Lymphatic vessels remove and transport excess interstitial fluid to lymph nodes (LNs) for balance immune protection. LNs are typically surrounded by perinodal adipose tissue (PAT). However, PAT is a blood vessel–rich but lymphatic-rare tissue; therefore, how in removed remains unclear. Using C57BL/6 mice, fluorescent dye tracing transmission electron microscopy results suggest that can travel the LN via collagen I+ channels (PAT-LN conduits), merge into collagen-rich space between...
Abstract Blood vascular endothelial cells (BECs) control the immune response by regulating cell recruitment, metabolite exchange and blood flow in lymphoid tissues. However, diversity of BEC their origins during angiogenesis remain poorly understood. Here we profile transcriptomes from mouse peripheral lymph nodes map key phenotypes to vasculature. Our analysis identifies multiple novel subsets including a venous population whose gene signature predicts an unexpectedly selective role myeloid...