Abstract Background The soil-borne fungal pathogen Verticillium dahliae Kleb causes wilt in a wide range of crops including cotton ( Gossypium hirsutum ). To date, most upland varieties are susceptible to V. and the breeding for with resistance has not been successful. Results Hpa1 Xoo is harpin protein from Xanthomonas oryzae pv. which induces hypersensitive cell death plants. When hpa1 was transformed into line Z35 through Agrobacterium -mediated transformation, transgenic (T-34) an...

The pestalotiopsis-like taxa, inclusive of Pseudopestalotiopsis, Pestalotiopsis, and Neopestalotiopsis, constitute a well-recognized fungal family. Recently, species within the Neopestalotiopsis genus have been identified as plant pathogens affecting crops fruits. However, there is currently paucity reference genome data for cubana in germplasm resource evaluation research. In this study, we present chromosome-level N. cubana, constructed using DNBSEQ platform PacBio RS II platform....

The exocyst complex in eukaryotic cells modulates secretory vesicle transportation to promote exocytosis. is also required for the hyphal growth and pathogenic development of several filamentous phytopathogens. Obligate biotrophic powdery mildew fungi cause considerable damage many cash crops; however, exocyst’s roles this group not well studied. To verify functions fungus, we identified two subunits, EqSec5 EqSec6, from Erysiphe quercicola, a fungus that infects rubber tree Hevea...

Bacillus bacteria can produce abundant secondary metabolites that are useful for biocontrol, especially in maintaining plant root microecology, and protection. In this study, we determine the indicators of six strains colonization, promotion growth, antimicrobial activity, other aspects, with aim obtaining a compound bacteriological agent to construct beneficial microbial community roots. We found there was no significant difference growth curves over 12 h. However, strain HN-2 have...

The transcriptome profile in leaves and roots of the transgenic cotton line T-34 expressing hpa1Xoo from Xanthomonas oryzae pv. was analysed using a customized 12k cDNA microarray. A total 530 transcripts involved 34 pathways were differentially expressed T-34, which 123 genes related to defence responses including hypersensitive reaction, associated with recognition pathogen-derived elicitors, signalling mediated by salicylic acid, jasmonic ethylene, auxin, abscicic Ca2+. Furthermore,...

Areca palm (Areca catechu), one of the two most important commercial crops in Hainan, China, has been severely damaged by a variety pathogens and insects. Here, we report new disease, tentatively referred to as areca necrotic ringspot disease (ANRSD), which is highly epidemic main growing regions Hainan. Transmission electron microscopy observation small RNA deep sequencing revealed existence viral agent family Potyviridae diseased plant (XC1). The virus was named (ANRSV). Subsequently,...

Soft rot pectobacteria are devastating plant pathogens with a global distribution and broad host range. Pectobacterium aroidearum L6, previously isolated from leaves of Syngonium podophyllum , is pectolytic bacterial pathogen that causes typical soft on S. . There shortage for genome data P. which seriously hinders research classification pathogenesis We present here the complete sequence L6. The L6 strain carries single 4,995,896-bp chromosome 53.10% G + C content harbors 4,306 predicted...

Abstract Background Harpins are proteins secreted by the type III secretion system of Gram-negative bacteria during pathogen–plant interactions that can act as elicitors, stimulating defense and plant growth in many types non-host plants. Harpin-treated plants have higher resistance, quality yields and, therefore, harpin may potentially valuable agricultural applications. characterized high thermal stability at 100 °C. However, it is unknown whether harpins still active temperatures above °C...

Abstract Harpin proteins are produced by plant-pathogenic Gram-negative bacteria and regulate bacterial pathogenicity inducing plant growth defence responses in non-hosts. HpaG-Xcm, a novel harpin protein, was identified from Xanthomonas citri pv. mangiferaeindicae , which causes black spot of mango. Here, we describe the predicted structure functions HpaG-Xcm investigate mechanism heat resistance. The amino acid sequence contains seven motifs two α-helices, N- C-terminals, respectively....

The pepper cultivar Yellow Lantern, one of the spiciest varieties, is a local germplasm Capsicum chinense, cultivated exclusively on Hainan Island, China. However, this variety susceptible to viral diseases that severely affect its production. In study, we report veinal mottle virus (PVMV) associated with foliar chlorosis and rugosity symptoms in Lantern. To verify correlation, constructed full-length cDNA clone PVMV isolate named HNu. progeny derived from replicated moved systemically...

Abstract Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants by delivering repertoire type III effectors. In the present study, we determined that deletion effector RipAF1 resulted in increased virulence on Nicotiana benthamiana, Solanum lycopersicum, and Capsicum annuum plants. showed ADP-ribosylation activity vivo vitro. Transient overexpression suppressed jasmonic acid (JA) signaling induced salicylic (SA) signaling. The was essential for JA...

Background Pepper veinal mottle virus (PVMV) belongs to the genus Potyvirus within family Potyviridae and is a major threat pepper production, causing reduction in yield fruit quality; however, efficient pesticides chemical treatments for plant protection against viral infections are lacking. Hence, there critical need discover highly active environment-friendly antiviral agents derived from natural sources. Bacillus spp. widely utilized as biocontrol manage fungal, bacterial, diseases....

The Potyviridae represent the largest group of known plant RNA viruses and account for more than half viral crop damage worldwide. leader proteases within family vary greatly in size arrangement play key roles during infection. Here, we experimentally demonstrate presence a distinct pattern proteases, HCPro1 HCPro2 tandem, newly identified member family. Moreover, HCPro2, which are closely related typically characterized with short size, have evolved contrasting silencing suppression...

Harpins, encoded by hrp (hypersensitive response and pathogenicity) genes of Gram-negative plant pathogens, are elicitors hypersensitive (HR). HpaXm is a novel harpin-like protein described from cotton leaf blight bacteria, Xanthomonas citri subsp. malvacearum-a synonym X. campestris pv. malvacearum (Smith 1901-1978). A putative signal peptide (1-MNSLNTQIGANSSFL-15) hpaXm was predicted in the nitroxyl-terminal (N-terminal)by SignalP (SignalP 3.0 server). Here, we explored function N-terminal...

Cucumber (Cucumis sativus L.) is one of the most important vegetables cultivated in world. It widely and mostly grown under greenhouse conditions (Sallam et al. 2021). has a long growth cycle particularly susceptible to bacterial diseases. In May 2021, leaf spot was found on cucumbers variety Lyuyou NO.3 Hainan Province, China. early stage disease, leaves showed small yellow-brown spots shape water stains. When exposed light, yellow halo around disease could be seen. later stages, lesions...