A5018124191- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Chromosomal and Genetic Variations
- Wnt/β-catenin signaling in development and cancer
Northwestern University
2018-2020
Significance Cilia are composed of hundreds proteins whose identities and functions far from being completely understood. In this study, we determined that calmodulin-regulated spectrin-associated protein 3 (CAMSAP3) plays an important role for the function motile cilia in multiciliated cells (MCCs). Global knockdown CAMSAP3 expression mice resulted defects ciliary structures, polarity, synchronized beating MCCs. These animals also displayed signs symptoms reminiscent primary dyskinesia...
Most epithelial cells polarize along the axis of tissue, a feature known as planar cell polarity (PCP). The initiation PCP requires cell–cell signaling via noncanonical Wnt/PCP pathway. Additionally, changes in cytoskeleton both facilitate and reflect this polarity. We have identified CLAMP/Spef1 novel regulator signaling. In addition to decorating microtubules (MTs) ciliary rootlet, pool CLAMP localizes at apical cortex. Depletion leads loss protein asymmetry, defects cilia polarity, angle...
Abstract 2‐Amino‐4‐(3,4‐[methylenedioxy]benzylamino)‐6‐(3‐methoxyphenyl)pyrimidine (AMBMP) is a small molecule that has been previously reported to be both Wnt agonist and microtubule (MT) regulator. Here we report detailed analysis of AMBMPs effects on MTs MT associated cellular processes including cell polarity, ciliogenesis, migration. Specifically, treatment Xenopus embryos with AMBMP leads defects similar the depolymerizing drug nocodazole, failure generate or polarize cilia (depending...