A5077240615- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Plant and Fungal Interactions Research
- Mosquito-borne diseases and control
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- Plant Molecular Biology Research
- Virology and Viral Diseases
- Chromosomal and Genetic Variations
- Viral Infections and Outbreaks Research
- Immune Cell Function and Interaction
- Insect and Pesticide Research
- Transgenic Plants and Applications
- Hepatitis C virus research
- Bamboo properties and applications
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Viral Infections and Vectors
University of Kentucky
2019-2024
Plant (United States)
2024
Fujian Medical University
2022
Fujian Agriculture and Forestry University
2014-2021
Summary Positive‐strand RNA viruses co‐opt organellar membranes for biogenesis of viral replication organelles (VROs). Tombusviruses also pro‐viral cytosolic proteins to VROs. It is currently not known what type molecular organization keeps co‐opted sequestered within membranous In this study, we employed tomato bushy stunt virus (TBSV) and carnation Italian ringspot (CIRV) – Nicotiana benthamiana pathosystems identify biomolecular condensate formation in We show that TBSV p33 the CIRV p36...
Elaborate viral replication organelles (VROs) are formed to support positive-strand RNA virus in infected cells. VRO formation requires subversion of intracellular membranes by proteins. Here, we showed that the key ATG8f autophagy protein and NBR1 selective receptor were co-opted Tomato bushy stunt (TBSV) closely-related carnation Italian ringspot virus. Knockdown or plants led reduced tombusvirus replication, suggesting pro-viral function for autophagy. BiFC proximity-labeling experiments...
Positive-strand RNA [(+)RNA] viruses assemble numerous membrane-bound viral replicase complexes (VRCs) with the help of replication proteins and co-opted host within large compartments in cytosol infected cells. In this study, we found that deletion or depletion Sac1 phosphatidylinositol 4-phosphate [PI(4)P] phosphatase reduced tomato bushy stunt virus (TBSV) yeast (Saccharomyces cerevisiae) plants. We demonstrate a critical role for TBSV assembly cell-free reconstitution assay. The effect...
The viral replication proteins of plus-stranded RNA viruses orchestrate the biogenesis large compartments, including numerous replicase complexes, which represent sites replication. formation and operation these virus-driven structures require subversion cellular proteins, membrane deformation, proliferation, changes in lipid composition hijacked membranes intensive synthesis. These processes plentiful ATP molecular building blocks produced at or delivered there. To obtain necessary...
Plus-stranded RNA viruses have limited coding capacity and to co-opt numerous pro-viral host factors support their replication. Many of the co-opted biogenesis viral replication compartments formation replicase complexes on subverted subcellular membrane surfaces. Tomato bushy stunt virus (TBSV) exploits peroxisomal membranes, whereas closely-related carnation Italian ringspot (CIRV) hijacks outer membranes mitochondria. How these organellar can be recruited into roles is not completely...
Positive-strand (+)RNA viruses take advantage of the host cells by subverting a long list protein factors and transport vesicles cellular organelles to build membranous viral replication (VROs) that support robust RNA replication. How accomplish major recruitment tasks large number proteins are intensively studied. In case tomato bushy stunt virus (TBSV), single protein, named p33, carries out most duties. Yet, it is currently unknown how p33 which membrane associated, capable rapid...
Positive-strand RNA viruses induce the biogenesis of viral replication organelles (VROs), which support in infected cells. VRO formation requires proteins, co-opted host factors and intracellular membranes. Here, we show that conserved Atg11 autophagy scaffold protein is by Tomato bushy stunt virus (TBSV) via direct interactions with proteins. Deletion ATG11 yeast or knockdown homologous plants led to reduced tombusvirus replication, thus indicating pro-viral function for Atg11. Based on...
Abstract Positive-strand RNA viruses co-opt intracellular organellar membranes for the biogenesis of viral replication organelles (VROs). The membranous VROs are sites replication. Tombusviruses numerous host proteins, many them cytosolic, not membrane-bound, such as glycolytic and fermentation enzymes. It is currently known what type molecular organization keeps these enzymes enriched sequestered within VROs. Here, we show evidence that tomato bushy stunt virus (TBSV) p33 closely-related...
CD7 and CD57 are related to the differentiation functional stages of CD8+ T cells. However, role their combined presence in cells patients with chronic hepatitis B virus (HBV) infection, especially those end-stage liver disease, remains unclear. Blood samples from healthy volunteers were analyzed via Luminex assay ELISA measure plasma cytokine levels. Further, recombinant IL-22 was used stimulate peripheral blood mononuclear volunteers, frequency CD3+ CD4- CD7+ CD57- apoptosis rates...