A5036354523- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- Nuclear Structure and Function
- Plant Virus Research Studies
- ATP Synthase and ATPases Research
Max Planck Institute for Molecular Genetics
2024
Institute of Molecular Biotechnology
2015-2021
Austrian Academy of Sciences
2015-2021
Vienna Biocenter
2015-2021
Medical University of Vienna
2021
Eötvös Loránd University
2015
Hungarian Academy of Sciences
2012-2015
The repression of transposable elements in eukaryotes often involves their transcriptional silencing via targeted chromatin modifications. In animal gonads, nuclear Argonaute proteins the PIWI clade complexed with small guide RNAs (piRNAs) serve as sequence specificity determinants this process. How binding PIWI–piRNA complexes to nascent transcripts orchestrates heterochromatin formation and is unknown. Here, we characterize CG9754/Silencio an essential piRNA pathway factor that required...
Base-excision repair and control of nucleotide pools safe-guard against permanent uracil accumulation in DNA relying on two key enzymes: uracil–DNA glycosylase dUTPase. Lack the major UNG gene from fruit fly genome dUTPase larvae prompted hypotheses that i) may accumulate Drosophila genomic where it be well tolerated, ii) this affect development. Here we show melanogaster tolerates high levels DNA; such is correctly interpreted cell culture embryo; iii) under physiological spatio-temporal...
Nuclear Argonaute proteins, guided by their bound small RNAs to nascent target transcripts, mediate cotranscriptional silencing of transposons and repetitive genomic loci through heterochromatin formation. The molecular mechanisms involved in this process are incompletely understood. Here, we show that the SFiNX complex, a mediator downstream from nuclear Piwi-piRNA complexes Drosophila , facilitates as homodimer. dynein light chain protein Cut up/LC8 mediates dimerization, its function can...
Despite a distinct developmental origin, extraembryonic cells in mice contribute to gut endoderm and converge transcriptionally resemble their embryonic counterparts. Notably, all progenitors share non-canonical epigenome, raising several pertinent questions, including whether this landscape is reset match the regulation if persist into later development. Here we developed two-colour lineage-tracing strategy track isolate over time. We find that display substantial memory of origin retention...
dUTP pyrophosphatase (dUTPase) is a dNTP-sanitizing enzyme that prevents the appearance of potentially harmful uracil bases in DNA by hydrolyzing cellular dUTP. This function dUTPase found to be essential many organisms including Drosophila melanogaster. Previously, we showed expression pattern determines extent accumulation genome different tissues. We wished find regulatory mechanism eventually leaves set tissues with uracil-free and intact genome. established promoter overlaps mRNA...
ABSTRACT The PIWI-interacting RNA (piRNA) pathway protects animal genome integrity in part through establishing repressive heterochromatin at transposon loci. Silencing requires piRNA-guided targeting of nuclear PIWI proteins to nascent transcripts, yet the subsequent molecular events are not understood. Here, we identify SFiNX (Silencing Factor interacting Nuclear eXport variant), an interdependent protein complex required for Piwi-mediated co-transcriptional silencing Drosophila . consists...
Abstract Nuclear Argonaute proteins, guided to nascent target RNAs by their bound small RNAs, elicit co-transcriptional silencing through heterochromatin formation at transposon insertions and repetitive genomic loci. The molecular mechanisms involved in this process are incompletely understood. Here, we propose that the SFiNX complex, a mediator downstream of nuclear Piwi-piRNA complexes Drosophila , enables via condensates. Condensate is stimulated nucleic acid binding requires...