A5051718868- Cellular Mechanics and Interactions
- Invertebrate Immune Response Mechanisms
- RNA Research and Splicing
- Insect symbiosis and bacterial influences
- Nuclear Structure and Function
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Signaling Pathways in Disease
- Neurobiology and Insect Physiology Research
- TGF-β signaling in diseases
- DNA Repair Mechanisms
- Cellular transport and secretion
- Heat shock proteins research
- Hippo pathway signaling and YAP/TAZ
- Protein Degradation and Inhibitors
- Insect Resistance and Genetics
- Immunotherapy and Immune Responses
- Skin and Cellular Biology Research
- Protein Tyrosine Phosphatases
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Connective Tissue Growth Factor Research
- Aquaculture disease management and microbiota
- Endoplasmic Reticulum Stress and Disease
Institute of Genetics
1998-2024
HUN-REN Szegedi Biológiai Kutatóközpont
2012-2024
Hungarian Academy of Sciences
2003-2019
University of California, Irvine
2001-2005
We have identified a previously undescribed transmembrane protein, Hemese, from Drosophila melanogaster blood cells (hemocytes), by using monoclonal pan-hemocyte antibody. Heavy glycosylation is suggested the heterogeneous size distribution, ranging between 37 and 70 kDa. Hemese expression restricted to cell surfaces of hemocytes all classes, hematopoietic organs. The sequence corresponding gene, ( He ), predicts glycophorin-like protein 15 kDa, excluding an N-terminal signal peptide, with...
Base-excision repair and control of nucleotide pools safe-guard against permanent uracil accumulation in DNA relying on two key enzymes: uracil–DNA glycosylase dUTPase. Lack the major UNG gene from fruit fly genome dUTPase larvae prompted hypotheses that i) may accumulate Drosophila genomic where it be well tolerated, ii) this affect development. Here we show melanogaster tolerates high levels DNA; such is correctly interpreted cell culture embryo; iii) under physiological spatio-temporal...
The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report molecular analysis computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that mir-282 gene is source 4.9-kb-long primary transcript with 5' cap and 3'-poly(A) sequence mature ∼25 bp. Our data strongly suggest existence an independent conserved holometabolic insects. give evidence locus encodes functional...
Protein phosphatase 4 (PP4) is an evolutionarily conserved and essential Ser/Thr that regulates cell division, development DNA repair in eukaryotes. The major form of PP4, present from yeast to human, the PP4c-R2-R3 heterotrimeric complex. R3 subunit responsible for substrate-recognition via its EVH1 domain. In typical domains, phenylalanine, tyrosine tryptophan residues specific recognition site their target's proline-rich sequences. Here, we identify novel binding partners domain...
ABSTRACT Dorsal closure of the Drosophila embryonic epithelium provides an excellent model system for in vivo analysis molecular mechanisms regulating cytoskeletal rearrangements. In this study, we investigated function spectraplakin Short stop (Shot), a conserved structural protein, during dorsal epithelium. We show that Shot is essential efficient final zippering opposing epithelial margins. By using isoform-specific mutant alleles and genetic rescue experiments with truncated variants,...
<i>Aims</i>—To analyse the similarities between Twisted gastrulation (TSG) proteins known to date; in addition, determine phylogenetic relations among TSG proteins, and TSGs other protein families—the CCN (for example, CCN2 (CTGF), CCN1 (CYR61), CCN3 (NOV)) IGFBP (insulin-like growth factor binding protein) families. <i>Methods</i>—TBLASTN FASTA3 were used identify new tsg genes relatives of family. The sequences aligned with ClustalW. predictions sites for signal peptide cleavage,...
The conserved Ser/Thr protein phosphatase 5 (PP5) is involved in the regulation of key cellular processes, including DNA damage repair and cell division eukaryotes. As a co-chaperone Hsp90, PP5 has been shown to modulate maturation activity numerous oncogenic kinases. Here, we identify novel substrate PP5, Polo-like kinase 4 (Plk4), which master regulator centriole duplication animal cells. We show that specifically interacts with Plk4, able dephosphorylate vitro vivo, affects interaction...
Morphogenesis, the establishment of animal body, requires coordinated rearrangement cells and tissues regulated by a very strictly-determined genetic program. Dorsal closure epithelium in Drosophila melanogaster embryo is one best models for such complex morphogenetic event. To explore regulation dorsal closure, we carried out large-scale RNA interference-based screen combination with vivo time-lapse microscopy identified several genes essential or affecting its dynamics. One novel genes,...
Abstract Ezrin‐Radixin‐Moesin proteins are highly conserved, actin‐binding cytoskeletal that play an essential role in microvilli formation, T‐cell activation, and tumor metastasis by linking actin filaments to the plasma membrane. Recent studies demonstrated only protein of Drosophila melanogaster , Moesin, is involved mitotic spindle function through stabilizing cell shape microtubules at cortex. We previously observed Moesin localizes spindle; hence, we tested for biological significance...
Actin, as an ancient and fundamental protein, participates in various cytoplasmic well nuclear functions eukaryotic cells. Based on its manifold tasks the nucleus, it is a reasonable assumption that presence of actin essential for cell, consequently, localization ensured by robust system. However, today only single import export pathway known which maintain dynamic balance between pools. In our work, we tested robustness actin, investigated whether perturbations affect viability whole...
The ERM protein family, which consists of three closely related proteins in vertebrates, ezrin, radixin, and moesin (ERM), is an ancient important group cytoplasmic actin-binding organizing proteins. With their FERM domain, ERMs bind various transmembrane anchor them to the actin cortex through C-terminal F-actin binding thus they are major regulators dynamics cell. participate many fundamental cellular processes, such as phagocytosis, microvilli formation, T-cell activation tumor...
The members of the evolutionary conserved actin-binding Ezrin, Radixin and Moesin (ERM) protein family are involved in numerous key cellular processes cytoplasm. In last decades, ERM proteins, like actin other cytoskeletal components, have also been shown to be functional components nucleus; however, molecular mechanism behind their nuclear activities remained unclear. Therefore, our primary aim was identify interactome single