A5034698353- Cellular Mechanics and Interactions
- Developmental Biology and Gene Regulation
- Cardiomyopathy and Myosin Studies
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Neurobiology and Insect Physiology Research
- Muscle Physiology and Disorders
- Epigenetics and DNA Methylation
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Chromosomal and Genetic Variations
- Cellular transport and secretion
- RNA Research and Splicing
- Plant Molecular Biology Research
- Advanced Electron Microscopy Techniques and Applications
- Genetic Neurodegenerative Diseases
- Autophagy in Disease and Therapy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Histone Deacetylase Inhibitors Research
- Genetics, Aging, and Longevity in Model Organisms
- Immune cells in cancer
- Physiological and biochemical adaptations
- Axon Guidance and Neuronal Signaling
- Invertebrate Immune Response Mechanisms
University of Szeged
2019-2024
Institute of Genetics
2015-2024
HUN-REN Szegedi Biológiai Kutatóközpont
2015-2024
Hungarian Academy of Sciences
2012-2023
Hungarian Research Network
2022
Eötvös József Főiskola
2017
University of Pecs
2015
University of Geneva
1990-2001
European Molecular Biology Organization
2001
European Molecular Biology Laboratory
2000
Summary Parasegmental (PS)-specific expression of the homeotic genes bithorax-complex (BX-C) appears to depend upon subdivision complex into a series functionally independent cis-regulatory domains. Fab-7 is regulatory element that lies between iab-6 and iab-7 (the PS11- PS12-specific domains, respectively). Deletion causes ectopic iab- 7 in PS11 (where normally only active). Two models have been proposed account for dominant phenotype. The first considers functions as boundary insulates...
The Drosophila bithorax complex Abdominal-B (Abd-B) gene specifies parasegmental identity at the posterior end of fly. specific pattern Abd-B expression in each parasegment (PS) determines its and, PS10-13, is controlled by four parasegment-specific cis-regulatory domains, iab-5 to iab-8, respectively. In order properly determine identity, these domains must function autonomously during both initiation and maintenance phases BX-C regulation. studies reported here demonstrate that...
In the work reported here we have undertaken a functional dissection of Polycomb response element (PRE) from iab-7 cis-regulatory domain Drosophila melanogasterbithorax complex (BX-C). Previous studies mapped iab-7PRE to an 860-bp fragment located just distal Fab-7boundary. Located within this is ∼230-bp chromatin-specific nuclease-hypersensitive region called HS3. We shown that HS3 capable functioning as Polycomb-dependent silencer in vivo, inducing pairing-dependent silencing amini-white...
Formins are involved in a wide range of cellular processes that require the remodeling actin cytoskeleton. Here, we have analyzed novel Drosophila formin, belonging to recently described DAAM subfamily. In contrast previous assumptions, show DAAMplays no essential role planar cell polarity signaling, but it has striking requirements organizing apical cables define taenidial fold pattern tracheal cuticle. These observations provide evidence first time function organization is prevent collapse...
The regulation of growth cone actin dynamics is a critical aspect axonal control. Among the proteins that are directly involved in dynamics, nucleation factors play pivotal role by promoting formation novel filaments. However, essential developing neurons have so far not been clearly identified. Here, we show expression data, and use true loss-of-function analysis targeted activated constructs to demonstrate Drosophila formin DAAM plays morphogenesis. In agreement with this finding, dDAAM...
The PI3K/Akt signaling pathway, Notch, and other oncogenes cooperate in the induction of aggressive cancers. Elucidating how pathway facilitates tumorigenesis by may offer opportunities to develop drugs with fewer side effects than those currently available. Here, using an unbiased vivo chemical genetic screen Drosophila, we identified compounds that inhibit activity proinflammatory enzymes nitric oxide synthase (NOS) lipoxygenase (LOX) as selective suppressors Notch-PI3K/Akt cooperative...
Wnt-Frizzled (Fz) signaling pathways play recurring important roles during the development and homeostasis of vertebrates invertebrates. Fz receptors can signal through β-catenin–dependent –independent pathways. In Drosophila , Fz2 are redundant for Wg. addition, conveys signals a distinct pathway to organize planar polarization epithelial structures. We demonstrate that cytoplasmic sequences preferentially activate β-catenin polarity cascade, respectively. Both either pathway, but with...
The three homeotic genes of the bithorax complex (BX-C), Ubx,abd-A and Abd-B control identity posterior thorax all abdominal segments. Large segment-specific cis-regulatory regions expression Ubx, abd-A or in each These span whole 300 kb BX-C are arranged on chromosome same order as segments they specify. Experiments with lacZ reporter constructs revealed existence several types regulatory elements regions. include initiation elements, maintenance cell type- tissue-specific enhancers,...
C-150 a Mannich-type curcumin derivative, exhibited pronounced cytotoxic effects against eight glioma cell lines at micromolar concentrations. Inhibition of proliferation by was mediated affecting multiple targets as confirmed transcription and protein level. effectively reduced the activation NFkB, inhibited PKC-alpha which are constitutively over-expressed in glioblastoma. The on Akt/ Notch signaling were also demonstrated Drosophila tumorigenesis model. number tumors with similar efficacy...
Recent studies established that the planar cell polarity (PCP) pathway is critical for various aspects of nervous system development and function, including axonal guidance. Although it seems clear PCP signaling regulates actin dynamics, mechanisms through which this occurs remain elusive. Here, we establish a functional link between one specific regulator, formin DAAM, has previously been shown to be required embryonic morphogenesis filopodia formation in growth cone. We show <i>dDAAM</i>...
Directed axonal growth depends on correct coordination of the actin and microtubule cytoskeleton in cone. However, despite relatively large number proteins implicated actin-microtubule crosstalk, mechanisms whereby polymerization is coupled to stabilization advancement peripheral cone remained largely unclear. Here, we identified formin Dishevelled-associated activator morphogenesis (DAAM) as a novel factor playing role concerted regulation remodeling Drosophilamelanogaster primary neurons....
Abstract The Abd-B gene, one of the three homeotic genes in Drosophila bithorax complex (BX-C), is required for proper identity fifth through eighth abdominal segments (corresponding to parasegments 10–14) fruitfly. morphological difference between these four due differential expression Abd-B, which achieved by action parasegment-specific cisregulatory regions infra-abdominal-5 (iab-5), -6, -7 and -8. dominant gain-of-function mutation Frontabdominal-7 (Fab-7) removes a boundary separating...
During muscle development, myosin and actin containing filaments assemble into the highly organized sarcomeric structure critical for function. Although sarcomerogenesis clearly involves de novo formation of filaments, this process remained poorly understood. Here we show that mouse Drosophila members DAAM formin family are sarcomere-associated assembly factors enriched at Z-disc M-band. Analysis dDAAM mutants revealed a pivotal role in myofibrillogenesis larval somatic muscles, indirect...
The indirect flight muscle is a widely used model for studying sarcomere structure and development due to its extremely regular architecture. Nevertheless, precise measurement of the basic sarcomeric parameters remains challenge even in this greatly ordered tissue. In study, we identified several factors affecting reliability developed software tool precise, high-throughput length myofibril width. accuracy new was validated against simulated images blinded manual measurements. To extend...
We characterized the properties of Drosophila melanogaster DAAM-FH2 and DAAM-FH1-FH2 fragments their interactions with actin profilin by using various biophysical methods in vivo experiments. The results show that although fragment does not have any conspicuous effect on assembly vivo, cells expressing fragment, a profilin-dependent increase formation structures is observed. trachea-specific expression also induces phenotypic effects, leading to collapse tracheal tube lethality larval...
F-actin networks are important structural determinants of cell shape and morphogenesis. They regulated through a number actin-binding proteins. The function many these proteins is well understood, but very little known about how they cooperate integrate their activities in cellular contexts. Here, we have focussed on the roles actin regulators controlling filopodial dynamics. Filopodia needle-shaped, actin-driven protrusions with characteristic features that conserved amongst vertebrates...
Sarcomeres are extremely highly ordered macromolecular assemblies where structural organization is intimately linked to their functionality as contractile units. Although the basis of actin and Myosin interaction revealed at a quasiatomic resolution, much less known about molecular I-band H-zone. We report development powerful nanoscopic approach, combined with structure-averaging algorithm, that allowed us determine position 27 sarcomeric proteins in Drosophila melanogaster flight muscles...
During heart formation, a network of transcription factors and signaling pathways guide cardiac cell fate differentiation, but the genetic mechanisms orchestrating assembly lumen formation remain unclear. Here, we show that small GTPase Cdc42 is essential for Drosophila melanogaster morphogenesis formation. genetically interacts with cardiogenic factor tinman; dDAAM which belongs to family actin organizing formins; zipper, encodes nonmuscle myosin II. Zipper required its spatiotemporal...