A5027984451- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Neuroscience and Neuropharmacology Research
- Lipid metabolism and biosynthesis
- Cancer Immunotherapy and Biomarkers
- Endoplasmic Reticulum Stress and Disease
- Neurological diseases and metabolism
- Cardiac electrophysiology and arrhythmias
- Cardiac Arrest and Resuscitation
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Sarcoma Diagnosis and Treatment
- Curcumin's Biomedical Applications
- Hydrogen's biological and therapeutic effects
- Protein Degradation and Inhibitors
- Cardiac Ischemia and Reperfusion
- Integrated Circuits and Semiconductor Failure Analysis
- Religious Tourism and Spaces
- Autoimmune Neurological Disorders and Treatments
- Cell Image Analysis Techniques
- Advanced Breast Cancer Therapies
- CAR-T cell therapy research
- Barrier Structure and Function Studies
- Protein Kinase Regulation and GTPase Signaling
University of Szeged
2001-2022
Hungarian Academy of Sciences
1996-2014
Institute of Biochemistry
2002-2013
Umicore (Belgium)
2011-2013
University of Debrecen
2008
HUN-REN Szegedi Biológiai Kutatóközpont
1998
C-150 a Mannich-type curcumin derivative, exhibited pronounced cytotoxic effects against eight glioma cell lines at micromolar concentrations. Inhibition of proliferation by was mediated affecting multiple targets as confirmed transcription and protein level. effectively reduced the activation NFkB, inhibited PKC-alpha which are constitutively over-expressed in glioblastoma. The on Akt/ Notch signaling were also demonstrated Drosophila tumorigenesis model. number tumors with similar efficacy...
Endomorphins were subjected to a number of structural modifications in search for their bioactive conformations. The alicyclic β-amino acids cis-(1S,2R)ACPC/ACHC, cis-(1R,2S)ACPC/ACHC, trans-(1S,2S)ACPC/ACHC, and trans-(1R,2R)ACPC/ACHC introduced into endomorphins examine the conformational effects on bioactivity. Use combination receptor binding techniques, 1H NMR, molecular modeling allowed conclusion that Pro2 substitution by these residues causes changes structure, proteolytic stability,...
Cytoplasmic lipid-droplets are common inclusions of eukaryotic cells. Lipid-droplet binding thalidomide analogs (2,6-dialkylphenyl-4/5-amino-substituted-5,6,7-trifluorophthalimides) with potent anticancer activities were synthesized.Cytotoxicity was detected in different cell lines including melanoma, leukemia, hepatocellular carcinoma, glioblastoma at micromolar concentrations. The synthesized non-toxic to adult animals up 1 g/kg but teratogenic zebrafish embryos concentrations defects the...
Background: A deleterious, late-onset side effect of thoracic radiotherapy is the development radiation-induced heart disease (RIHD). It covers a spectrum cardiac pathology including also failure with preserved ejection fraction (HFpEF) characterized by left ventricular hypertrophy (LVH) and diastolic dysfunction. MicroRNA-212 (miR-212) crucial regulator pathologic LVH via FOXO3-mediated pathways in pressure-overload-induced failure. We aimed to investigate whether miR-212 its selected...
Prolonged exposure to opioid agonists can induce adaptive changes resulting in tolerance and dependence. Here, rats were rendered tolerant by subcutaneous injections of increasing doses morphine from 10 60 mg/kg for 3, 5, or consecutive days. Binding parameters the μ-opioid receptor subcellular fractions measured with [<sup>3</sup>H]DAMGO ([d-Ala<sup>2</sup>,<i>N</i>-Me-Phe<sup>4</sup>,Gly<sup>5</sup>-ol]-enkephalin). Although density surface μ-sites did not change after 5-day treatment,...
Toxicogenomics, based on the temporal effects of drugs gene expression, is able to predict toxic earlier than traditional technologies by analyzing changes in genomic biomarkers that could precede subsequent protein translation and initiation histological organ damage. In present study our objective was extend vivo toxicogenomic screening from one or a few tissues multiple organs, including heart, kidney, brain, liver spleen. Nanocapillary quantitative real-time PCR (QRT-PCR) used study, due...
Hepatocellular carcinoma (HCC) is the most frequent and aggressive primary tumor of liver it has limited treatment options.In this study, we report in vitro vivo effects two novel amino-trifluoro-phtalimide analogs, Ac-915 Ac-2010. Both compounds bind lipid droplets endoplasmic reticulum membrane, interact with several proteins chaperone functions (HSP60, HSP70, HSP90, protein disulfide isomerase) as determined by affinity chromatography resonant waveguide optical biosensor technology....
Background: Reperfusion of ischemic myocardium may contribute to substantial cardiac tissue damage, but the addition iron chelators, zinc or complexes has been shown prevent heart from reperfusion injury. We investigated possible beneficial effects an iron-chelating and zinc-complexing agent, Q50, in rat models ischemia/reperfusion (I/R)-induced myocardial infarction on global reversible I/R injury after transplantation. Methods Results: Rats underwent 45-min ischemia by left anterior...
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from 14,585-member library further tested on living cells under microscope. Eleven compartment-specific, cell-permeable (or plasma...
Since the discovery of endomorphins (EM), postulated endogenous peptide agonists mu-opioid receptors, several analogues have been synthesized to improve their binding and pharmacological profiles. We shown previously that a new analogue, cis-1S,2R-aminocyclohexanecarboxylic acid(2)-endomorphin-2 (ACHC-EM2), had elevated mu-receptor affinity, selectivity, proteolytic stability over parent compound. In present work, we studied its antinociceptive effects receptor regulatory processes. ACHC-EM2...
To establish a transborder virtual tumor board (VTB) fostering state-of-the-art management of cancer patients by exchanging knowledge and expertise among oncologists in Central Southeastern Europe (CEE).We established implemented VTB based on the WebEx platform. This allowed for password-protected secure upload patient cases to be presented discussed colleagues from various oncology centers scattered throughout CEE order arrive at recommendation further diagnoses and/or treatment.A total 73...
Here we have studied regulatory changes of µ-opioid receptors accompanying in vivo 14-methoxymetopon treatments rats. Previously, this ligand has been shown to be an extremely potent, centrally acting specific analgesic with low physical dependence, tolerance, respiratory depression, constipation and other side effects. Our work shows that it is a highly potent full agonist receptor coupled G-protein signaling vitro, alike the well-known opioid agonist, etorphine. However, unlike etorphine,...
AbstractAbstractWe have investigated the thermodynamic parameters of various opioid ligands interacting with their receptors in rat brain membranes. Affinity constants (Ka), enthalpy and entropy values were obtained from homologous displacement experiments performed at 0, 24 33°C. It was found that all agonists tested ([3H]dihydromorphine (DHM) μ alkaloid; [3H]DAMGO peptide; [3H]deltorphin-B δ peptide) display endothermic binding accompanied a large increase, regardless chemical structure...
Event Abstract Back to Hydrogen membrane transport activity coupled with changing deuterium/hydrogen ratio may be a key proliferation signal for the cells Gábor Somlyai1*, Lajos Nagy2, László Puskás2, Gabriella Fábián2, Zoltán Gyöngyi3, Krisztina Krempels1, Ildikó Somlyai1, Laskay4, Jancsó5, György Jákli5, András Kovács6, Imre Guller6, Daiana S. Ávila7 and Michael Aschner7 1 HYD Ltd. Cancer Research Drug Development, Hungary 2 Avidin Biotechnology Ltd., 3 University of Pécs, 4 Department...