A5078188940- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Isotope Analysis in Ecology
- Radioactive element chemistry and processing
- Cancer-related Molecular Pathways
- Radioactive contamination and transfer
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Mesenchymal stem cell research
- Analytical chemistry methods development
- Cancer, Lipids, and Metabolism
- Extracellular vesicles in disease
- Estrogen and related hormone effects
- Radioactivity and Radon Measurements
- Galectins and Cancer Biology
- Nuclear Physics and Applications
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Advanced Breast Cancer Therapies
International Atomic Energy Agency
2013-2023
HUN-REN Szegedi Biológiai Kutatóközpont
2010-2021
Institute of Genetics
2010-2021
Seibersdorf Laboratories (Austria)
2019
Hungarian Academy of Sciences
2009-2019
FAO/IAEA Agriculture and Biotechnology Laboratories
2019
Institute of Biochemistry
2017
Accenture (Switzerland)
2005
Abstract Background PGRMC1 (progesterone receptor membrane component 1) is a highly conserved heme binding protein, which overexpressed especially in hormone receptor-positive breast cancer and plays an important role carcinogenesis. Nevertheless, little known about the mechanisms by drives tumor progression. The aim of our study was to investigate involvement cholesterol metabolism detect new can increase lipid alter cancer-related signaling pathways leading Methods effect overexpression...
Recently, it has been described that programmed cell death protein 1 (PD-1) overexpressing melanoma cells are highly aggressive. However, until now not defined which factors lead to the generation of PD-1 subpopulations. Here, we present melanoma-derived exosomes, conveying oncogenic molecular reprogramming, induce formation a melanoma-like, population (mMSCPD-1+) from naïve mesenchymal stem (MSCs). Exosomes and mMSCPD-1+ tumor progression expression in vivo. Finally, revealed...
The synthesis and characterization of a novel redox responsive comb-copolymer consisting poly(ferrocenylsilane) backbone N-dimethylethyl ammonium N-dimethyldecyl substituents are reported. Due to the presence side groups featuring cationic amine as well decyl hydrocarbon chains comb copolymer exhibits amphiphilic behaviour forms micellar assemblies with typical dimensions 100 nm. display unique, induced morphology change in water, investigated by dynamic light scattering transmission...
There is convincing epidemiological and experimental evidence that capsaicin, a potent natural transient receptor potential cation channel vanilloid member 1 (TRPV1) agonist, has anticancer activity. However, capsaicin cannot be given systemically in large doses, because of its induction acute pain neurological inflammation. MRS1477, dihydropyridine derivative acts as positive allosteric modulator TRPV1, if added together with but ineffective, alone. Addition MRS1477 evoked Ca2+ signals MCF7...
ABSTRACT An in vivo approach has been developed for generation of artificial chromosomes, based on the induction intrinsic, large-scale amplification mechanisms mammalian cells. Here, we describe successful prototype human satellite DNA-based chromosomes via amplification-dependent de novo chromosome formations induced by integration exogenous DNA sequences into centromeric/rDNA regions acrocentric chromosomes. Subclones with mitotically stable were established, which allowed initial...
Bone marrow derived mesenchymal stromal cells (MSCs) have recently been implicated as one source of the tumor-associated stroma, which plays essential role in regulating tumor progression. In spite intensive research, individual factors MSCs controlling progression not adequately defined. present study we examined galectin-1 (Gal-1), a protein highly expressed tumors with poor prognosis, course development. Co-transplantation wild type 4T1 mouse breast carcinoma enhances incidence palpable...
Abstract Some seashells of the Philippine venus species and sea coral Porites sp. were studied by means FT‐Raman, Fourier transform infrared spectroscopy (FTIR) Far‐FTIR spectroscopic methods. The Raman spectra show that both P. are aragonite‐structured CaCO 3 . Detailed spectral analysis, however, reveals some small differences, due to differences in crystallite size or habit different minor element contents. IR contains also quantities calcite‐structured carbonates. ν 2 band (shoulder)...
The B6.Cg-Tg(Thy1-YFP)16Jrs/J transgenic mouse strain, widely used to study neuronal development and regeneration, expresses the yellow fluorescent protein (YFP) in peripheral nerves central nervous system under control of regulatory sequences Thy1 gene. (CD90) cell surface glycoprotein is present on many types besides neurons known be involved adhesion, migration signal transduction. We hypothesized that Thy1-activating conditions could probably activate truncated Thy1-YFP construct,...
Adipose-tissue stem cells (ASCs) are subject of intensive research since their successful use in regenerative therapy. The drawback ASCs is that they may serve as stroma for cancer and assist tumor progression. It disquieting frequently undergo genetic epigenetic changes during vitro propagation. In this study, we describe the polyploidization murine accompanying phenotypical, gene expressional functional under long term culturing. were isolated from visceral fat C57BL/6 J mice, cultured...
Toxicogenomics, based on the temporal effects of drugs gene expression, is able to predict toxic earlier than traditional technologies by analyzing changes in genomic biomarkers that could precede subsequent protein translation and initiation histological organ damage. In present study our objective was extend vivo toxicogenomic screening from one or a few tissues multiple organs, including heart, kidney, brain, liver spleen. Nanocapillary quantitative real-time PCR (QRT-PCR) used study, due...
The paper describes an analytical method for rapid categorization of nuclear forensic evidence based on uranium isotopic composition. investigation uranium-based materials seized in Hungary was carried out by inductively coupled plasma sector-field mass spectrometry (ICP-SFMS) applying laser ablation (LA) sample introduction. results obtained the LA-ICP-SFMS were compared with independent techniques. advantage and limitations are discussed.
Mammalian artificial chromosomes are natural chromosome-based vectors that may carry a vast amount of genetic material in terms both size and number. They reasonably stable segregate well mitosis meiosis. A platform chromosome expression system (ACEs) was earlier described with multiple loading sites for modified lambda-integrase enzyme. It has been shown this ACEs is suitable high-level industrial protein production the treatment mouse model devastating human disorder, Krabbe’s disease....
Background: The close proximity of adipose tissue and mammary epithelium predispose involvement cells in breast cancer development. Adipose-tissue stem (ASCs) contribute to tumor stroma promote growth cells. In our previous study, we have shown that murine ASCs, which undergo polyploidization during their prolonged vitro culturing, enhanced the proliferation 4T1 IGF1 dependent manner. Aims: present aim was clarify regulation ASC-derived IGF1. Methods: carcinoma were co-transplanted with...
MC-ICP-MS methods for accurate and precise determination of Pu amount isotopic composition at picogram to femtogram levels.