A5102021175- Cardiac Ischemia and Reperfusion
- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Nitric Oxide and Endothelin Effects
- Renal Transplantation Outcomes and Treatments
- Cardiac and Coronary Surgery Techniques
- Mesenchymal stem cell research
- Cardiovascular Function and Risk Factors
- Pancreatic function and diabetes
- Cardiac electrophysiology and arrhythmias
- Electrospun Nanofibers in Biomedical Applications
- Aortic Disease and Treatment Approaches
- Pulmonary Hypertension Research and Treatments
- Fuel Cells and Related Materials
- Cardiac Fibrosis and Remodeling
- Adenosine and Purinergic Signaling
- Phosphodiesterase function and regulation
- Diabetes Treatment and Management
- Cardiac Arrest and Resuscitation
- Mechanical Circulatory Support Devices
- Neurological Disease Mechanisms and Treatments
- Cardiovascular Issues in Pregnancy
- Sexual function and dysfunction studies
- PARP inhibition in cancer therapy
- Cerebrovascular and Carotid Artery Diseases
Heidelberg University
2015-2024
Martin Luther University Halle-Wittenberg
2020-2024
University Hospital Heidelberg
2010-2023
University Hospital in Halle
2020-2023
St. Josef-Hospital
2014-2021
Ruhr University Bochum
2014-2021
St. Elisabeth-Hospital Bochum
2018-2021
Heidelberg (Poland)
2018
Semmelweis University
2009-2018
Chettinad Health City
2016-2018
The sodium–glucose cotransporter-2 (SGLT2) inhibitor canagliflozin has been shown to reduce major cardiovascular events in type 2 diabetic patients, with a pronounced decrease hospitalization for heart failure (HF) especially those HF at baseline. These might indicate potent direct cardioprotective effect, which is currently incompletely understood. We sought characterize the effects of acute treatment healthy and infarcted rat hearts. Non-diabetic male rats were subjected sham operation or...
Background— The role of the nitric oxide/cGMP/cGMP–dependent protein kinase G pathway in myocardial protection and preconditioning has been object intensive investigations. novel soluble guanylate cyclase activator cinaciguat reported to elevate intracellular [cGMP] activate vivo. We investigated effects on infarction induced by isoproterenol rats. Methods Results— Rats were treated orally twice a day for 4 days with vehicle or (10 mg/kg). Isoproterenol (85 mg/kg) was injected subcutaneously...
Diabetes mellitus (DM) is associated with characteristic structural and functional changes of the myocardium, termed diabetic cardiomyopathy. As a distinct entity independent coronary atherosclerosis, cardiomyopathy an increasingly recognized cause heart failure. A detailed understanding cardiac dysfunction, using relevant animal models, required for effective prevention treatment cardiovascular complications in patients. We investigated compared performance rat models type 1 DM...
Background: Reports about the generation of 3-dimensional neoscaffolds for myocardial tissue engineering are limited. The architecture provided by perfusion decellularization whole hearts would support production human-sized living tissues from an acellular matrix. aim this study was to evaluate potential a model heart engineering. Methods and Results: Hearts were obtained 12 German Landrace pigs selected abattoir. After preparation, mounted perfused on modified Langendorff specifically...
Background and purpose: Patients with diabetes mellitus exhibit generalized endothelial cardiac dysfunction decreased nitric oxide production. Elevated intracellular cyclic guanosine monophosphate (cGMP) levels contribute to an effective cardioprotection in different pathophysiological conditions. In this study, we investigated whether chronic treatment the phosphodiesterase‐5 inhibitor vardenafil could improve diabetic cardiovascular by up‐regulating oxide–cGMP pathway vessel wall...
Background: Heart valve tissue engineering represents a concept for improving the current methods of valvular heart disease therapy. The aim this study was to develop engineered valves combining human umbilical vein endothelial cells (HUVECs) and decellularized matrices. Methods Results: Pulmonary (n=9) aortic (n=6) allografts were harvested from explanted hearts transplant recipients using detergent-based cell extraction method. Analysis decellularization success performed with light...
Vascular ischemia/reperfusion injury (IRI) contributes to graft failure and adverse clinical outcomes following coronary artery bypass grafting. Sodium-glucose-cotransporter (SGLT)-2-inhibitors have been shown protect against myocardial IRI, irrespective of diabetes. We hypothesized that adding canagliflozin (CANA) (an SGLT-2-inhibitor) saline protects vascular grafts from IRI. Aortic rings non-diabetic rats were isolated immediately mounted in organ bath chambers (control, n = 9–10 rats) or...
Increasing evidence suggests that both types of diabetes mellitus (DM) lead to cardiac structural and functional changes. In this study we investigated compared characteristics underlying subcellular pathological features in rat models type-1 type-2 diabetic cardiomyopathy. Type-1 DM was induced by streptozotocin. For DM, Zucker Diabetic Fatty (ZDF) rats were used. Left ventricular pressure-volume analysis performed assess function. Myocardial nitrotyrosine immunohistochemistry, TUNEL assay,...
Heart transplantation is the therapy of choice for end-stage heart failure. However, hemodynamic instability, which has been demonstrated in brain-dead donors (BDD), could also affect posttransplant graft function. We tested hypothesis that treatment BDD with dopamine derivate n-octanoyl-dopamine (NOD) improves donor cardiac and function after transplantation. Donor rats were given a continuous intravenous infusion either NOD (0.882 mg/kg/h, BDD+NOD, n = 6) or physiological saline vehicle...
Objectives: Phosphodiesterase-5 inhibitors and elevated myocardial cyclic guanosine monophosphate levels can induce potent cardioprotection-like effects against ischaemia–reperfusion injury. We investigated the of vardenafil, a selective phosphodiesterase-5 inhibitor on endothelial functions during reperfusion in canine model cardioplegic arrest extracorporal circulation. Methods: Vehicle-treated (control, n = 8) vardenafil-treated (30 μg kg−1 intravenous (IV); n= anaesthetised dogs...
Oxidative stress interferes with nitric oxide (NO)/soluble guanylate cyclase (sGC)/cyclic guanosine monophosphate (cGMP) signalling pathway through reduction of endogenous NO and formation the strong intermediate oxidant peroxynitrite leads to vascular dysfunction. We evaluated effects oral treatment NO- heme-independent sGC activator cinaciguat on peroxynitrite-induced dysfunction in rat aorta. Sprague-Dawley rats were treated orally 2 times at an interval 17 hours vehicle or (10 mg/kg)....
Background Many donor organs come from youths involved in alcohol-related accidental death. The use of cardiac allografts for transplantation donors after acute poisoning is still under discussion while ethanol intoxication associated with myocardial functional and morphological changes. aims this work were 1) to evaluate rats the time-course effects ethanol-exposure 2) explore how its abuse by might affect recipients pump function transplantation. Methods Rats received saline or (3.45 g/kg,...
Abstract Background and Purpose Olaparib, rucaparib niraparib, potent inhibitors of poly(ADP‐ribose) polymerase (PARP) are approved as anti‐cancer drugs in humans. Considering the previously demonstrated role PARP various forms acute chronic myocardial injury, we tested effects olaparib in‐vitro models oxidative stress cardiomyocytes, an vivo model cardiac transplantation. Experimental Approach H9c2‐embryonic rat heart‐derived myoblasts pretreated with vehicle or (10μM) were challenged...
Sex differences in pressure overload (PO)-induced left ventricular (LV) myocardial hypertrophy (LVH) have been intensely investigated. Nevertheless, sex-related disparities of LV hemodynamics LVH were not examined detail. Therefore, we aimed to provide a detailed characterization distinct aspects function male and female rats during different stages LVH. Banding the abdominal aorta (AB) was performed induce PO for 6 or 12 wk rats. Control animals underwent sham operation. The development...
Abstract Background Brain death (BD) has been suggested to induce coronary endothelial dysfunction. Ischemia/reperfusion (IR) injury during heart transplantation may lead further damage of the endothelium. Previous studies have shown protective effects conditioned medium (CM) from bone marrow-derived mesenchymal stem cells (MSCs) against IR injury. We hypothesized that physiological saline-supplemented CM protects BD rats’ vascular grafts Methods The rat MSCs, used for conservation purposes,...
Investigating the effect of sex on pressure unloading therapy in a clinical scenario is limited by several nonstandardized factors. Hence, we sought to study sex-related similarities and differences under laboratory conditions. Pressure overload was induced male female rats aortic banding (AB) for 6 12 wk. Age-matched sham-operated animals served as controls. performed debanding at week 6. Different aspects myocardial remodeling were characterized echocardiography, pressure-volume analysis,...
Heart transplantation has become the most effective treatment for end-stage heart failure. Donors after brain death (BD) are currently only reliable source cardiac transplants. However, haemodynamic instability and dysfunction have been demonstrated in brain-dead donors this could therefore also affect post-transplant graft function. We studied effects of BD on function its short-term (1 h) or long-term (5 impacts In Lewis rats, was induced by inflation a subdurally placed balloon catheter...