A5062488498- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- Signaling Pathways in Disease
- Neurobiology and Insect Physiology Research
- Botulinum Toxin and Related Neurological Disorders
- Developmental Biology and Gene Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Invertebrate Immune Response Mechanisms
- Temporomandibular Joint Disorders
- GABA and Rice Research
- Lysosomal Storage Disorders Research
- Hippo pathway signaling and YAP/TAZ
- Endoplasmic Reticulum Stress and Disease
- Wnt/β-catenin signaling in development and cancer
- Medicinal Plants and Neuroprotection
- Nuclear Structure and Function
- Oropharyngeal Anatomy and Pathologies
- S100 Proteins and Annexins
- Craniofacial Disorders and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Ubiquitin and proteasome pathways
- Neurological disorders and treatments
- Trypanosoma species research and implications
- Hereditary Neurological Disorders
Rikkyo University
2013-2024
Minophagen Pharmaceutical (Japan)
2024
Chukyo University
2024
Tohoku University
1997-2022
Yamaguchi University
2022
Aomori Prefectural Industrial Technology Center
2019-2021
Apple (Israel)
2019-2021
Showa University
2019
Apple (United States)
2019
Waseda University
2008-2018
Abstract: Polyclonal antibodies against Ca 2+ /calmodulin‐de‐pendent protein kinase II (CaM II) of rat brain were prepared by immunizing rabbits and then purified antigen‐affinity column. The which recognized both sub‐units the enzyme with M r S 49K 60K used for study on distribution CaM in formalin‐fixed, paraffin‐embedded tissues. In brain, a light‐microscopic demonstrated strong immunoreactivity neu‐ronal somata dendrites weak nuclei. densely stained regions included cerebral cortex,...
Abstract: Isolated microtubule‐associated protein 2 (MAP2), τ factor, and tubulin were phosphorylated by a purified Ca 2+ , calmodulin‐dependent kinase (640K enzyme) from rat brain. The phosphorylation of MAP2 factor separately induced the inhibition microtubule assembly, in accordance with degree. Tubulin 640K enzyme whereas effect catalytic subunit was undetectable. effects MAPs on assembly greater than that either or phosphorylation. Because MAP2, also type‐II cyclic AMP‐dependent brain,...
Abstract: Calcineurin dephosphorylated microtubule‐associated protein 2 (MAP2) and τ factor phosphorylated by cyclic AMP‐dependent Ca 2+ , calmodulin‐dependent kinases from the brain. Tubulin, only kinase, served as substrate for calcineurin. The concentrations of calmodulin required to give half‐maximal activation calcineurin were 21 16 n M with MAP2 substrates, respectively. K m V max values in ranges 1–3 μ 0.4–1.7 μmol/mg/ min, respectively, factor. value tubulin was a similar range, but...
In this paper, we provide direct evidence that glutathione S-transferase pi (GSTpi) detoxifies cisplatin (CDDP). We used human colonic cancer HCT8 cells sensitive and resistant to CDDP, the level of cisplatin-glutathione adduct (DDP-GSH) being higher in cells. There was an overexpression GSTpi mRNA these CDDP-resistant Incubation with CDDP resulted formation DDP-GSH dependent on concentration incubation time. The abolished when were pre-treated ethacrynic acid or ketoprofen, inhibitors...
ABSTRACT Lactobacilli with tannase activity were isolated from human feces and fermented foods. A PCR-based taxonomic assay revealed that the isolates belong to Lactobacillus plantarum , L. paraplantarum pentosus . Additional studies on a range of species established culture collections confirmed this enzymatic is phenotypic property common these three species.
p38 mitogen-activated protein kinase (p38) has been extensively studied as a stress-responsive kinase, but its role in development remains unknown. The fruit fly, Drosophila melanogaster, two genes, D-p38a and D-p38b. To elucidate the developmental function of p38's, we used various genetic pharmacological manipulations to interfere with their functions: expression dominant-negative form D-p38b, antisense D-p38b RNA, reduction D-p38 gene dosage, treatment inhibitor SB203580. Expression wing...
Little has been understood about the underlying mechanisms that generate morphological diversity of dendritic trees. Dendritic arborization neurons in Drosophila provide an excellent model system to tackle this question, and they are classified into classes I-IV order increasing arbor complexity. Here we have developed transgenic green fluorescent protein markers for class I or IV cells, which allowed time-lapse recordings birth embryo, its maturation processes larva, lesion-induced...
Nucleotide sulfate, namely 3′-phosphoadenosine 5′-phosphosulfate (PAPS), is a universal sulfuryl donor for sulfation. Although specific PAPS transporter present in Golgi membrane, no study has reported the corresponding gene. We have identified novel human gene encoding transporter, which we named PAPST1, and Drosophila melanogaster ortholog, slalom (sll). The amino acid sequence of PAPST1 (432 acids) exhibited 48.1% identity with SLL (465 acids), hydropathy analysis predicted two to be type...
✓ Results in three patients with thrombosed giant aneurysms of the vertebral artery are reported. Each presented as a mass lesion. On postcontrast computerized tomography and magnetic resonance imaging, each aneurysm demonstrated patent lumen intrathrombotic vascular channels. Two died were autopsied, other patient was successfully treated. Pathological examination revealed that had staged clots, an open lumen, channels endothelial lining, aneurysmal walls intimal thickening. The authors...
A topographical immunocytochemical analysis was performed on the substantia nigra from patients with idiopathic Parkinson's disease and striatonigral degeneration. Antibodies to tyrosine hydroxylase, a marker for nigrostriatal dopaminergic neurons, calcineurin, projection fibers, were used in this study. There marked depletion of neurons parkinsonian compared control subjects, reduction being greater lateral portion than medial (p less 0.001). Calcineurin immunoreactivity densely distributed...
Abstract Knowledge on the distribution and dynamics of glycosylation enzymes in Golgi is essential for better understanding this modification. Here, using a combination CRISPR/Cas9 knockin technology super-resolution microscopy, we show that complex assembled by number small ‘Golgi units’ have 1-3 μm diameter. Each unit contains domains which call ‘zones’. The zones N- O-glycosylation are colocalised. However, they less colocalised with glycosaminoglycan synthesizing enzyme. units change...
A striking variety of glycosylation occur in the Golgi complex a protein-specific manner, but how this diversity and specificity are achieved remains unclear. Here we show that stacked fragments (units) dispersed Drosophila imaginal disk cells functionally diverse. The UDP-sugar transporter FRINGE-CONNECTION (FRC) is localized to subset units distinct from those harboring SULFATELESS (SFL), which modifies glucosaminoglycans (GAGs), protease RHOMBOID (RHO), processes glycoprotein SPITZ (SPI)....
The increased resolution of Quad Full High Definition (QFHD) offers significantly enhanced visual experience. However, the corresponding huge data throughput up to 530 Mpixels/s greatly challenges design real-time video decoder VLSI with extensive requirement on both DRAM bandwidth and computational power. In this work, a lossless frame recompression technique partial MB reordering scheme are proposed save access QFHD chip. Besides, pipelining parallelization techniques such as...
ABSTRACT Two thoracic limbs of Drosophila, the leg and wing, originate from a common cluster cells that include source two secreted signaling molecules, Decapentaplegic Wingless. We show Wingless, but not Decapentaplegic, is responsible for initial specification limb primordia with distal identity. Limb formation restricted to lateral position embryo by negative control early function EGF receptor homolog determine global dorsoventral pattern. Late locally determines additional cell...
Abstract Meige syndrome is an adult‐onset dystonic movement disorder that predominantly involves facial muscles, while some patients with this develop spasmodic dysphonia and dystonia of the neck, trunk, arms, legs. We report all symptoms had been refractory to both pharmacotherapy bilateral thalamotomy were markedly alleviated by pallidal stimulation in a patient segmental axial advanced from syndrome. © 2001 Movement Disorder Society.
The mammalian striatum arises in the basal telencephalon and contains morphologically homogenous neurons that can be divided into two distinct compartments, patches matrix. During development, patch are generated first to form a striatal primordium. After large influx of later-born matrix this region, unique mosaic arrangement these neuronal phenotypes is established. massive migration continues during embryonic period, they eventually comprise 80-85% mature striatum. To elucidate cellular...
r-GCS andGlutathione S-Conjugate Transport 20367shown ( 14), and some characteristics of the enzyme were reported (15).However, physiological role this has not been fully understood.K562 cells are known to differentiate into erythroid after addition erythropoietin (EP) (16).On other hand, it that HSP family is involved i n cell differentiation proliferation (17), still relationship between cycle clarified.In present study, we investigated metabolism y-GCS transport glutathione S-conjugate...
Recent studies have demonstrated protective roles for autophagy in various neurodegenerative disorders, including the polyglutamine diseases; however, role of retinal degeneration has remained unclear. Accumulation activated rhodopsin some Drosophila mutants leads to degeneration, and although it is known that degraded endosomal pathways normal photoreceptor cells, contribution regulation elusive. This study reveals by collaboration with prevent degeneration. Light-dependent visual system...