A5090382563- Plant Micronutrient Interactions and Effects
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Spectroscopy and Chemometric Analyses
- Receptor Mechanisms and Signaling
- Biochemical and Molecular Research
- Sugarcane Cultivation and Processing
- Spectroscopy Techniques in Biomedical and Chemical Research
- Metabolomics and Mass Spectrometry Studies
- Protein Structure and Dynamics
- Neuroblastoma Research and Treatments
- Gene expression and cancer classification
- Immunotherapy and Immune Responses
- Asymmetric Hydrogenation and Catalysis
- Advanced NMR Techniques and Applications
- Metalloenzymes and iron-sulfur proteins
- Inorganic and Organometallic Chemistry
- Glycosylation and Glycoproteins Research
- Viral gastroenteritis research and epidemiology
- Analytical Chemistry and Chromatography
- Advanced MRI Techniques and Applications
- Click Chemistry and Applications
- Infectious Encephalopathies and Encephalitis
- Chemical Synthesis and Analysis
- SARS-CoV-2 and COVID-19 Research
University of Oxford
2021-2025
Rosalind Franklin Institute
2022-2024
Max Planck Institute for Terrestrial Microbiology
2024
Bioscience Research
2024
Many pathogens exploit host cell-surface glycans. However, precise analyses of glycan ligands binding with heavily modified pathogen proteins can be confounded by overlapping sugar signals and/or compounded known experimental constraints. Universal saturation transfer analysis (uSTA) builds on existing nuclear magnetic resonance spectroscopy to provide an automated workflow for quantitating protein-ligand interactions. uSTA reveals that early-pandemic, B-origin-lineage severe acute...
Abstract To extract information from NMR experiments, users need to identify the number of resonances in spectrum, together with characteristic features such as chemical shifts and intensities. In many applications, particularly those involving biomolecules, this procedure is typically a manual laborious process. While algorithms are available tackle problem, their performance tends be inferior that an experienced user. Here, we introduce UnidecNMR, which identifies spectra using...
Abstract Boron is absent in proteins, yet a micronutrient. It possesses unique bonding that could expand biological function including modes of Lewis acidity not available to typical elements life. Here we show post-translational Cβ–Bγ bond formation provides mild, direct, site-selective access the minimally sized residue boronoalanine (Bal) proteins. Precise anchoring boron within complex biomolecular systems allows dative bond-mediated, site-dependent protein acid–base-pairing (LABP) by...
Biomolecule environments can enhance chemistries with the potential to mediate and modulate self-modification (e.g., self-cleavage). While these enhanced modes are found in certain biomolecules RNA ribozymes), it is more rare proteins. Targeted proteolytic cleavage vital physiology, biotechnology, even emerging therapy. Yet, purely chemically induced methods for site-selective of proteins remain scarce. Here, as a proof principle, we designed tested system intended combine protein-enhanced...
Abstract Ganglioside sugars, as Tumour-Associated Carbohydrate Antigens (TACAs), are long-proposed targets for vaccination and therapeutic antibody production, but their self-like character imparts immunorecessive characteristics that classical approaches have to date failed overcome. One prominent TACA, the glycan component of ganglioside GM3 (GM3g), is over-expressed on diverse tumours. To probe limits tolerance, we used protein editing methods display GM3g in systematically varied...
Abstract Many host pathogen interactions such as human viruses (including non-SARS-coronaviruses) rely on attachment to cell-surface glycans. There are conflicting reports about whether the Spike protein of SARS-CoV-2 binds sialic acid commonly found N-linked In absence a biochemical assay, ability analyze binding glycans heavily- modified proteins and resolve this issue is limited. Classical Saturation Transfer Difference (STD) NMR can be confounded by overlapping sugar resonances that...
Abstract All NMR experiments require sequences of RF ‘pulses’ to manipulate nuclear spins. Signal is lost due non-uniform excitation spins resonating at different energies (chemical shifts) and inhomogeneity in the actually generated by hardware over sample volume. Here, we present Seedless, a tool calculate pulses that compensate for these effects enhance control magnetisation boost signal. The calculations take only few seconds using an optimised GRadient Ascent Pulse Engineering (GRAPE)...
Abstract Many archetypal and emerging classes of small-molecule therapeutics form covalent protein adducts. In vivo, both the resulting conjugates their off-target side-conjugates have potential to elicit antibodies, with implications for allergy drug sequestration. Although β-lactam antibiotics are a class long associated these immunological phenomena, molecular underpinnings drug-protein conjugation consequent drug-specific immune responses remain incomplete. Here, using classical...
Abstract Methylthio-alkane reductases convert methylated sulfur compounds to methanethiol and small hydrocarbons, a process with important environmental biotechnological implications. These enzymes are classified as nitrogenase-like enzymes, despite lacking the ability dinitrogen ammonia, raising fundamental questions about factors controlling their activity specificity. Here, we present first molecular structure of methylthio-alkane reductase, which reveals large metalloclusters, including...
T. S. Patterson, C. Buchanan and A. J. Summers, Chem. Soc., 1941, 606 DOI: 10.1039/JR9410000606
Abstract Many classes of small-molecule drugs form protein adducts in vivo , which may elicit antibodies via a classical hapten-carrier-type response, with implications for both allergy and drug sequestration. Although β-lactam antibiotics are class long associated these phenomena, the molecular determinants drug-protein conjugation consequent drug-specific immune responses remain incomplete. Here, we interrogated factors influencing penicilloyl adduct formation immunogenicity, used...
Abstract To extract information from NMR experiments, users need to identify the number of resonances in spectrum,together with characteristic features such as chemical shiftsand intensities. In many applications, particularly those involving biomolecules, this procedure is typicallya manual and laborious process. While manyalgorithms are available tackle problem, theirperformance tends be inferior that an experienced user.Here,weintroduce UnidecNMR, which identifies spectra using...