A5028923514- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Synthesis and Biological Evaluation
- Parasitic Infections and Diagnostics
- Helminth infection and control
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Parasite Biology and Host Interactions
- Cancer-related gene regulation
- Legionella and Acanthamoeba research
- Insect symbiosis and bacterial influences
- Urticaria and Related Conditions
- Vector-borne infectious diseases
- Parasitic infections in humans and animals
- Parasitic Diseases Research and Treatment
- Toxin Mechanisms and Immunotoxins
- Cardiomyopathy and Myosin Studies
Autonomous University of Yucatán
2013-2025
Texas Children's Hospital
2020-2023
Baylor College of Medicine
2020-2023
Chagas disease is a chronic infection caused by the protozoan parasite, Trypanosoma cruzi, with limited benefits of currently available anti-parasitic chemotherapeutic approaches to halt progression heart disease. Recombinant TSA-1-C4 and Tc24-C4 proteins have been developed as promising antigen candidates for therapeutic vaccines, leading propose them in combination bivalent recombinant protein strategy. In this study, we evaluated immunomodulatory effect combined vitro assays using murine...
Dogs play a crucial role in the transmission cycle of Trypanosoma cruzi and their presence domicile increases risk infection humans. In Yucatán, Mexico previous studies have reported T. dogs from both rural urban areas, which we expanded here, to better understand dynamics. A total 186-dogs were screened for by PCR serology. Parasite burden, genotype, immune response, cardiac alterations, roaming behavior analyzed. The prevalence was 26.8% (50/186). Genotyping revealed predominance TcI...
Chagas disease, caused by the protozoan Trypanosoma cruzi (T. cruzi), is most significant neglected tropical disease affecting individuals in Americas. Currently, available drugs, such as nifurtimox and benznidazole (BZN), are both toxic ineffective chronic phase of disease. A promising alternative development a vaccine, although this effort hampered complexity parasite HLA polymorphisms. In addition, activation epitope-specific CD8+ T cells critical to conferring robust cell-mediated immune...
A therapeutic vaccine for human Chagas disease (American trypanosomiasis caused by Trypanosoma cruzi) is under development based on the success of vaccinating mice with DNA constructs expressing antigens Tc24 and Tc-TSA-1. However, because nucleic acid vaccines produce less than optimal responses in humans, our strategy relies administering a recombinant protein-based vaccine, together adjuvants that promote Th1-type immunity. Here we describe process purification refolding TSA-1 expressed...
The diversity of Trypanosoma cruzi parasites infecting humans is still poorly understood. We used deep sequencing to analyze this in chagasic patients from Mexico. Such information crucial understand transmission cycles and identify determinants epidemiological clinical characteristics the infection. analyzed parasite mini-exon spliced-leader sequences following amplification blood DNA by polymerase chain reaction sequencing. Chagasic presented a diverse assemblage haplotypes covering TcI,...
Abstract Chagas disease resulting from Trypanosoma cruzi infection leads to a silent, long-lasting chronic neglected tropical affecting the poorest and underserved populations around world. Antiparasitic treatment with benznidazole does not prevent progression or death in patients established cardiac disease. Our consortium is developing therapeutic vaccine based on T. flagellar—derived antigen Tc24-C4 formulated Toll-like receptor 4 agonist adjuvant, complement existing chemotherapy improve...
Trypanosoma cruzi antigens TSA-1 and Tc24 have shown promise as vaccine candidates in animal studies. We evaluated here the recall immune response these induce Chagasic patients, a first step to test their immunogenicity humans. vitro cellular after stimulation with recombinant (rTSA-1) or (rTc24) mononuclear cells of asymptomatic chronic patients (n = 20) compared healthy volunteers 19) from Yucatan, Mexico. Proliferation assays, intracellular cytokine staining, cytometric bead arrays,...
We discuss the potential usefulness of molecular testing soil, dust, and water samples to detect medically important parasites, where such could be used supplement stool sampling in humans. A wide variety parasites including protozoa helminths, many which are zoonotic, have an infection reservoir environment. In some cases, this environmental period is essential for further parasite development. describe progress implementing methods detection these soil across eight collaborating centers...
Background Chagas disease (CD) is caused by Trypanosoma cruzi and affects 6–7 million people worldwide. Approximately 30% of chronic patients develop chagasic cardiomyopathy (CCC) after decades. Benznidazole (BNZ), one the first-line chemotherapy used for CD, induces toxicity fails to halt progression CCC in patients. The recombinant parasite-derived antigens, including Tc24, Tc24-C4, TSA-1, TSA-1-C4 with Toll-like receptor 4 (TLR-4) agonist-adjuvants reduce cardiac parasite burdens, heart...
Abstract Introduction Chagas disease, caused by the protozoan parasite Trypanosoma cruzi , is a major public health problem in Americas, and existing drugs have severe limitations. In this context, vaccine would be an attractive alternative for disease control. One of difficulties developing effective lies high genetic diversity T. . study, we evaluated level sequence leading candidate Tc24 multiple strains. Methods Results We quantified its polymorphism within between discrete typing units...
Cutaneous leishmaniasis is a tropical disease affecting over one million patients annually and Leishmania (L.) mexicana of the major etiological agents in Americas. Here we established first experimental infection L. canids. Beagle dogs were infected intradermally with culture-derived mexicana. We followed skin ulcer development, histopathological signs, parasite burden immune status dogs. All developed uniform oval-craterform ulcers similar to those observed humans, associated mixed T...
About 6.5 million people worldwide are afflicted by Chagas disease, which is caused the protozoan parasite Trypanosoma cruzi. The development of a therapeutic vaccine to prevent progression Chagasic cardiomyopathy has been proposed as an alternative for antiparasitic chemotherapy. Bioinformatics tools can predict MHC class I CD8 + epitopes inclusion in single recombinant protein with goal develop multivalent vaccine. We expressed novel Tc24-C4.10E harboring ten nonameric and using Tc24-C4...
E6020 is a synthetic agonist of Toll-like receptor-4 (TLR4). The purpose this study was to evaluate the effect different doses E6020-SE on Trypanosoma cruzi-specific immune responses and its ability confer protection against acute lethal infection in mice. Forty female BALB/c were infected with 500 trypomastigotes T cruzi H1 strain, divided into four groups (n = 10) treated at 7- 14-day post-infection (dpi) or PBS (control). Survival followed for 51 days, mice euthanized hearts collected...
Peromyscus yucatanicus (Rodentia: Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida: Trypanosomatidae). Nitric oxide (NO) generally plays crucial role in the containment and elimination Leishmania. The aim this study was to determine amount NO produced by P. infected with L. (L.) mexicana. Subclinical clinical infections were established through inoculation 1 x 10 2 2.5 6 promastigotes, respectively. Peritoneal macrophages cultured alone or co-cultured...
Abstract. Although mesenchymal stem cells (MSCs) are the major source of adipocytes, adipogenesis is a highly complex process whose mechanisms driving adipocyte origin and development remain poorly understood. Previous findings by our group have shown that different MSCs from oral cavity displayed differential expression TET2, key regulator DNA methylation, during adipogenic induction. Therefore, we proposed to evaluate effects overexpression TET2 on response cell line with low natural...
Abstract Background Chagas disease (CD) is caused by Trypanosoma cruzi and affects 6-7 million people worldwide. Approximately 30% of chronic patients develop chagasic cardiomyopathy (CCC) after decades. Benznidazole (BNZ), one the first-line chemotherapy approved for CD, induces toxicity fails to halt progression CCC in patients. The recombinant parasite-derived antigens, including Tc24, Tc24-C4, TSA-1, TSA-1-C4 with Toll-like receptor 4 (TLR-4) agonist-adjuvants reduce cardiac parasite...
The soil is the primary environmental reservoir for many parasites transmitted to humans that cause disease. Our study used a multiparallel real-time quantitative polymerase chain reaction assay detect parasite DNA in collected from outdoor built environments of 34 houses rural Yucatan, Mexico. number positive ( n , %) per species was 18 (53%) Acanthamoeba spp.; four (12%) Blastocystis spp. and Ascaris lumbricoides ; three (9%) Toxocara canis one (3%) Ancylostoma spp., Trichuris trichiura...
ABSTRACT The soil is the primary environmental reservoir for many parasites transmitted to humans through fecal-oral route, causing disease. Our study used a high-throughput multi-parallel real-time quantitative PCR (qPCR) assay detect parasites’ DNA in collected from outdoor built environments of 34 houses. total parasite prevalence was Acanthamoeba spp . (53%), Blastocystis spp. (12%), Ascaris lumbricoides Toxocara canis (9%), Ancylostoma (3%), Trichuris trichiura Entamoeba histolytica...
Chagas disease is a chronic infection caused by Trypanosoma cruzi . The first-line drugs approved for treatment have several limitations and are associated with toxicity. recombinant TSA-1-C4 Tc24-C4 proteins been profiled as promising candidates the formulation of therapeutic vaccines, leading us to propose them in combination bivalent protein strategy. In this study, we evaluated immunomodulatory effect plus in-vitro assays. Macrophages from naïve BALB/c mice were isolated stimulated...
Chagas disease by Trypanosoma cruzi (T. cruzi) infection is a leading cause of myocarditis worldwide. cardiomyopathy presented with wide variety conduction abnormalities including arrhythmias, first- and second-degree atrioventricular blockade, left ventricular systolic dysfunction some cases heart failure to the death. Currently, there are no effective treatments available against advanced disease. With advance in development novel therapies, it important utilize an animal model that can...