A5086071034- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Electron and X-Ray Spectroscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Algal biology and biofuel production
- Graphene and Nanomaterials Applications
- Bacterial Identification and Susceptibility Testing
- Advanced Electron Microscopy Techniques and Applications
- Carbon dioxide utilization in catalysis
- Ion-surface interactions and analysis
- Electrospun Nanofibers in Biomedical Applications
- Muscle metabolism and nutrition
- Microfluidic and Bio-sensing Technologies
- Bacterial biofilms and quorum sensing
- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Antimicrobial agents and applications
- Nanopore and Nanochannel Transport Studies
- Polysaccharides and Plant Cell Walls
- Microbial metabolism and enzyme function
- Nanoplatforms for cancer theranostics
- Microbial Fuel Cells and Bioremediation
- Infant Nutrition and Health
- Seaweed-derived Bioactive Compounds
- Computational and Text Analysis Methods
Czech Academy of Sciences, Institute of Scientific Instruments
2015-2024
Brno University of Technology
2014-2015
Accumulation of polyhydroxybutyrate (PHB) seems to be a common metabolic strategy adopted by many bacteria cope with cold environments. This work aimed at evaluating and understanding the cryoprotective effect PHB. At first monomer PHB, 3-hydroxybutyrate, was identified as potent cryoprotectant capable protecting model enzyme (lipase), yeast (Saccharomyces cerevisiae) bacterial cells (Cupriavidus necator) against adverse effects freezing-thawing cycles. Further, viability frozen–thawed PHB...
Abstract Correlative imaging, integrating diverse observational methods, has become increasingly vital for comprehensive sample analysis by linking distinct datasets that are accessible only through individual techniques. This study combines cryogenic scanning electron microscopy (cryo-SEM) and Raman micro-spectroscopy (cryo-Raman), which together offer extensive insight into analysis. Cryo-SEM effectively investigates the surface, structure, morphology, while cryo-Raman excels in detecting...
Many bacteria are capable of accumulating intracellular granules polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis bacterial cells to study changes in the diameters as well PHA during growth and accumulation bacterium Cupriavidus necator H16 (formerly Ralstonia eutropha). The cell envelope was stained by DiD® fluorescent probe Nile Red. Signals from both probes were separated based on their spectral fluorescence life-time properties. During accumulation,...
Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim this work was to evaluate the PHA potential a novel PHA-producing thermophilic Gram-positive isolate Aneurinibacillus sp. H1. This organism capable efficient conversion glycerol into poly(3-hydroxybutyrate) (P3HB), homopolyester 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature 45 °C,...
Polyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the forefront of interest due their biodegradability and production from renewable sources. A potential increase in competitiveness PHA process comes with a combination use thermophilic bacteria mutual waste substrates. In this work, bacterium Tepidimonas taiwanensis LMG 22826 was identified as promising producer. The ability produce T. studied both on genotype phenotype levels. gene encoding Class I synthase,...
The biofilm-forming microbial species Candida parapsilosis and Staphylococcus epidermidis have been recently linked to serious infections associated with implanted medical devices. We studied biofilms by high resolution scanning electron microscopy (SEM), which allowed us visualize the biofilm structure, including distribution of cells inside extracellular matrix areas surface adhesion. compared classical SEM (chemically fixed samples) cryogenic SEM, employs physical sample preparation based...
Aneurinibacillus sp. H1 is a promising, moderately thermophilic, novel Gram-positive bacterium capable of the biosynthesis polyhydroxyalkanoates (PHA) with tunable monomer composition. In particular, strain able to synthesize copolymers 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB) and 3-hydroxyvalerate (3HV) remarkably high 4HB 3HV fractions. this study we performed an in-depth material analysis PHA polymers produced by in order describe how composition affects fundamental structural...
A method for in vitro identification of individual bacterial cells is presented. The based on a combination optical tweezers spatial trapping and Raman microspectroscopy acquisition spectral "Raman fingerprints" obtained from the trapped cell. Here, spectra were taken biofilm-forming without influence an extracellular matrix compared with biofilm-negative cells. Results principal component analyses enabled us to distinguish between two strains Staphylococcus epidermidis. Thus, we propose...
This study introduces an original concept in the development of hydrogel materials for controlled release charged organic compounds based on semi-interpenetrating polymer networks composed by inert gel-forming component and interpenetrating linear polyelectrolyte with specific binding affinity towards carried active compound. As it is experimentally illustrated prototype hydrogels prepared from agarose interpenetrated poly(styrene sulfonate) (PSS) alginate (ALG), respectively, main benefit...
The cyanobacterial genus Synechocystis is of particular interest to science and industry because its efficient phototrophic metabolism, accumulation the polymer poly(3-hydroxybutyrate) (PHB) ability withstand or adapt adverse growing conditions. One such condition increased salinity that can be caused by recycled brackish water used in cultivation. While overall reduced growth expected response salt stress, other metabolic responses relevant efficiency production biomass PHB (or both) have...
Since polyhydroxyalkanoates (PHAs) belong among the most widespread storage materials within bacteria genus, it can be assumed that ability of PHAs accumulation represent significant advantage in natural environments. Of course, probably obvious is possibility to utilize when external carbon sources are depleted. Nevertheless, likely play much more complex role stress response bacteria. Therefore, aim this work was investigate possible influence PHA on physical properties cells and their...
Journal Article Cryo-SEM and Raman Spectroscopy Study of the Involvement Polyhydroxyalkanoates in Stress Response Bacteria Get access V Krzyzanek, Krzyzanek Institute Scientific Instruments ASCR, v.v.i., Brno, Czech Republic Search for other works by this author on: Oxford Academic Google Scholar K Hrubanova, Hrubanova O Samek, Samek S Obruca, Obruca Centre Material Research, Brno University Technology, I Marova, Marova Bernatova, Bernatova M Siler, Siler P Zemanek Microscopy Microanalysis,...
Selenium (Se) is an element with many commercial applications as well essential micronutrient. Dietary Se has antioxidant properties and it known to play a role in cancer prevention. However, the general population often suffers from deficiency. Green algae, such Chlorella vulgaris, cultivated Se-enriched environment may be used food supplement provide adequate levels of Se. We Raman microspectroscopy (RS) for fast, reliable, non-destructive measurement concentration living algal cells....
Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt growth conditions even extreme environments. Thus, they ideal organisms for the Next Generation Industrial Biotechnology concept reducing risk contamination by using naturally robust extremophiles. Unfortunately, potential PPB use in biotechnology is hampered missing knowledge on...
Poly(3-hydroxybutyrate) (PHB) is a biobased and biodegradable polymer with properties comparable to polypropylene therefore has the potential replace conventional plastics. PHB intracellularly accumulated by prokaryotic organisms. For cells functions manly as carbon energy source, but all possible of are still not known. Synechocystis (cyanobacteria) accumulates using light CO2 source. The main trigger for accumulation in cyanobacteria nitrogen phosphorous depletion simultaneous surplus...
The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for µCT. Using various independent modalities, we thoroughly characterized the PEG-AuNPs. Here, show that PEG-AuNPs are retained in blood and provide a high contrast high-resolution µCT imaging of vessels inner organs. biodistribution is prolonged circulation accumulation liver, spleen skin. AuNP skin resulted blue discoloration eyes whole In vitro experiments using leukemic...
Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With ability to fix CO2, cyanobacteria interesting for white biotechnology as cell factories produce various high-value metabolites such polyhydroxyalkanoates, pigments, or proteins. White is the industrial production processing chemicals, materials, energy using microorganisms. It known that exposing low levels stressors can induce secondary metabolites. Understanding this...