A5100773703- Epigenetics and DNA Methylation
- Electromagnetic wave absorption materials
- Renal and related cancers
- Advanced battery technologies research
- Metal-Organic Frameworks: Synthesis and Applications
- Electrocatalysts for Energy Conversion
- Advanced Antenna and Metasurface Technologies
- Chemical Synthesis and Analysis
- Animal Genetics and Reproduction
- CAR-T cell therapy research
- Click Chemistry and Applications
- Covalent Organic Framework Applications
- Membrane Separation and Gas Transport
- Supercapacitor Materials and Fabrication
- Radical Photochemical Reactions
- Peptidase Inhibition and Analysis
- Advanced Photocatalysis Techniques
- Metamaterials and Metasurfaces Applications
- Advancements in Battery Materials
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Crystallization and Solubility Studies
- Fluorine in Organic Chemistry
- Graphene research and applications
- X-ray Diffraction in Crystallography
Scripps Research Institute
2022-2025
Sun Yat-sen Memorial Hospital
2017-2025
Sun Yat-sen University
2011-2025
Zhejiang University of Technology
2024-2025
Jianghan University
2025
Shenzhen Children's Hospital
2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2019-2024
Chongqing Medical University
2019-2024
Scripps (United States)
2024
Scripps Institution of Oceanography
2024
We report a new reversible lysine conjugation that features novel diazaborine product and much slowed dissociation kinetics in comparison to the previously known iminoboronate chemistry. Incorporating diazaborine-forming warhead RMR1 peptide ligand gives potent long-acting covalent inhibitors of staphylococcal sortase. The efficacy sortase inhibition is demonstrated via biochemical cell-based assays. A comparative study an iminoboronate-forming highlights significance potential modulating...
How ion channels are gated to regulate flux in and out of cells is the subject intense interest. The Escherichia coli mechanosensitive channel, MscS, opens allow rapid efflux, relieving turgor pressure that would otherwise destroy cell. We present a 3.45 angstrom–resolution structure for MscS channel an open conformation. This has pore diameter ∼13 angstroms created by substantial rotational rearrangement three transmembrane helices. suggests molecular mechanism underlies gating its decay...
The interface is a critical issue for metal-organic-framework hybrid membranes in propylene separation. Here, covalent-linking strategy reported strikingly reinforcing the interfacial compatibility ZIF-8-based membrane. A functionalized ZIF-8 material named ZIF-8-CN synthesized using mixed-ligand approach. has an identical crystalline structure to ZIF-8, and 4,5-dicyanoimidazole ligand available further functionalization. Covalent linkage of with PIM-1 driven by thermal reaction cyano groups...
Gas separation efficiency of covalent organic framework (COF) membrane can be greatly elevated through precise functionalization. A pair-functionalized COF 1,3,5-triformylphloroglucinol (TP) and isoquinoline-5,8-diamine (IQD) monomers in two three nodes is designed synthesized. TP-IQD crystallized a two-dimensional structure with pore size 6.5 Å surface area 289 m2 g-1 . This possesses N-O paired groups which cooperatively interact C2 H2 instead H4 nanosheets ≈10 μm width ≈4 nm thickness are...
Covalent organic frameworks (COFs) mixed matrix membranes (MMMs) combining individual attributes of COFs and polymers are promising for gas separation. However, applying COF MMMs propylene/propane (C3 H6 /C3 H8 ) separation remains a big challenge due to inert pores C3 similar molecular sizes. Herein, the designed synthesis Cu(I) coordinated membrane is reported. A platform synthesized from 5,5'-diamino-2,2'-bipyridine 2-hydroxybenzene-1,3,5-tricarbaldehyde. This possesses porous 2D...
Abstract Triggering lysosome‐regulated immunogenic cell death (ICD, e.g., pyroptosis and necroptosis) with nanomedicines is an emerging approach for turning “immune‐cold” tumor “hot”—a key challenge faced by cancer immunotherapies. Proton sponge such as high‐molecular‐weight branched polyethylenimine (PEI) excellent at rupturing lysosomes, but its therapeutic application hindered uncontrollable toxicity due to fixed charge density poor understanding of resulted mechanism. Here, a series...
An efficient multicomponent reaction for the preparation of vinyl sulfone was achieved by hydrogenation/bifunctional sulfonylation alkynyl esters with N- allyl bromodifluoroacetamide, DABSO, and H 2 O under visible-light-only conditions.
Abstract The generation of alkyl radical from C(sp 3 )−H substrates via hydrogen atom abstraction represents a desirable yet underexplored strategy in alkylation reaction since involving common concerns remain adequately unaddressed, such as the harsh conditions, limited substrate scope, and employment noble metal- or photo-catalysts stoichiometric oxidants. Here, we utilize synergistic photoredox transfer (HAT) catalysis to accomplish general practical functionalization unactived centers...
Abstract We report a novel conjugation of N‐terminal cysteines (NCys) that proceeds with fast kinetics and exquisite selectivity, thereby enabling facile modification NCys‐bearing proteins in complex biological milieu. This new NCys via thiazolidine boronate (TzB) intermediate results from ( k 2 : ≈5000 m −1 s ) reversible 2‐formylphenylboronic acid (FPBA). designed FPBA derivative upon TzB formation elicits intramolecular acyl transfer to give N‐acyl thiazolidines. In contrast the quick...
FucoID is an intercellular proximity labeling technique for studying cell-cell interactions (CCIs) via fucosyltransferase (FT)-meditated fucosyl-biotinylation, which has been applied to probe antigen-specific dendritic cell (DC)-T interactions. In this system, bait cells of interest with surface-anchored FT are used capture the interacting prey by transferring a biotin-modified substrate cells. Here, we leveraged study CCIs directed different molecular pairs, e.g., programmed death...
Abstract Functionalized porous aromatic frameworks (PAFs) are excellent candidate materials for hybrid membrane fabrication. However, tailoring PAFs CO 2 separation with desirable performance is still a challenge. Here, facile fabrication of functional alkylamine‐modified PAF‐1 containing membranes high compatibility efficient /N reported. The methylamino groups installed on resulting in PAF‐1‐CH NH that has surface area over 1400 m g –1 and unique adsorption thermodynamic selectivity 1000....
Abstract The histone methyltransferase enhancer of zeste homolog 2 (EZH2) plays important roles in T-cell differentiation, proliferation and function. Previous studies have demonstrated that genetic deletion EZH2 CD8+ or total T cells impairs their antiviral antitumor activity, cytokine production ability to expand upon rechallenge. Contrary the detrimental role deleting cell–intrinsic EZH2, here we transient inhibition prior phenotypic onset exhaustion with a clinically approved inhibitor,...
<p>Figure S4 OT-I splenocytes were stimulated with 500 nM OVA257-264 for 3 days in the presence of 60 IU/mL IL2 and analyzed on day3.</p>
<p>Figure S3 OT-I splenocytes were stimulated with 500 nM OVA257-264 for 3 days in the presence of 60 IU/mL IL2 and expanded 2 more days. Taz treatment was started at 0h, 24h, 48h, 72h upon stimulation cells analyzed on day5.</p>
<p>Figure S8 Phenotype analysis of OT-I cells from B16-OVA tumor and EZH2 knockout provided a similar phenotype as tazemetostat inhibition but impaired survival upon restimulation.</p>
<p>Figure S6 EZH2 inhibition by tazemetostat during in vitro expansion enhanced vivo response of OT-I cells upon LM-OVA infection and improved long-term survival cells.</p>
<p>Figure S10 Transient inhibition of EZH2 by tazemetostat increased chemokine production OT-I cells.</p>
<p>Figure S2. Induction of EZH2 expression during human T cell stimulation.</p>
<p>Figure S11 Overlap of transcriptomic impact Ezh2 deletion vs Taz treatment on CD8 T Cells.</p>