A5100388125- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Advanced Biosensing Techniques and Applications
- Cell Image Analysis Techniques
- Electrochemical sensors and biosensors
- Electron Spin Resonance Studies
- Nanopore and Nanochannel Transport Studies
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Data Management and Algorithms
- Electrowetting and Microfluidic Technologies
- Diverse Aspects of Tourism Research
- Electromagnetic Fields and Biological Effects
- CRISPR and Genetic Engineering
- Human Mobility and Location-Based Analysis
- Advanced Fluorescence Microscopy Techniques
- Extraction and Separation Processes
- Ubiquitin and proteasome pathways
- Neuroscience and Neural Engineering
- Advanced Chemical Sensor Technologies
- RNA Interference and Gene Delivery
- Biopolymer Synthesis and Applications
Peking University
2010-2025
Ningbo Institute of Industrial Technology
2024
Chinese Academy of Sciences
2013-2024
Sichuan University
2019-2024
University of Chinese Academy of Sciences
2020-2024
Jiaxing University
2024
Zhejiang University of Technology
2024
State Key Laboratory of Food Science and Technology
2023
Jiangnan University
2023
Japan Agency for Marine-Earth Science and Technology
2019-2023
This work reports on a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. A numerical model is developed to simulate Dean flow inside curved geometry track particle/cell trajectories, which validated against experimental observations serves as theoretical foundation for optimizing operating conditions. Results separating cells (MCF-7 Hela) spiked...
The sustained liquid flow of a typical lateral assay can be mimicked by two-dimensional shaped, thin porous membranes, specifically rectangular membranes appended to circular sectors. In designing these fan-shaped devices, we have been aided analytical equations and finite-element simulations. We show both mathematically experimentally how continuous increase in unwetted pore volume causes deviation from traditional imbibition, leads quasi-stationary the element. These results are...
This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. assay integrates capillaries (glass or plastic) to introduce house sample/reagents, segments of water droplets prevent contamination, pocket warmers provide heat, hand-held flashlight visual readout the fluorescent signal. The cLAMP system allows simultaneous detection two RNA targets human immunodeficiency virus...
In this review, we summarize the separation mechanisms and materials adopted for fabrication of 2D material membranes as well their applications in critical separations.
This work develops an integrated microcapillary-based loop-mediated isothermal amplification (icLAMP) containing preloaded reagents and DNA extraction card, allowing for sample-to-answer screening of single nucleotide polymorphisms (SNPs) typing the CYP2C19 gene from untreated blood samples with minimal user operation. With all card inside capillary, this icLAMP system can achieve on-site pretreatment, extraction, amplification, detection nucleic acids within 150 min, without requirement...
Given the essential role of apurinic/apyrimidinic endonuclease (APE1) in gene repair and cancer progression, we report a novel approach for probing regulating cellular APE1 activity by using DNA tetrahedrons.
A strategy based on Poisson statistics enables a rapid and efficient acquisition of enzyme mutants from just few clones.
We established an ultrasensitive method for identifying enzymes in biological samples by multiplexed single-molecule enzymatic assay.
Rapid diagnosis based on naked-eye colorimetric detection remains challenging, but it could build new capacities for molecular point-of-care testing (POCT). In this study, we evaluated the performance of 16 types single-stranded DNA-fluorophore-quencher (ssDNA-FQ) reporters use with clusters regularly spaced short palindrome repeats (CRISPR)/Cas12a-based visual assays. Among them, nine ssDNA-FQ were found to be suitable direct detection, especially very strong using ROX-labeled reporters. We...
The usage of mobile phone increases globally. However, there is still a paucity data about the impact electromagnetic fields (EMF) on human health. This study investigated whether EMF radiation would alter biology glial cells and act as tumor-promoting agent. We exposed rat astrocytes C6 glioma to 1950-MHz TD-SCDMA for 12, 24 48 h respectively, found that exposure had differential effects astroctyes cells. A damaged mitochondria induced significant apoptosis astrocytes. Moreover, caspase-3,...
Betweenness centrality (BC) is widely used to identify critical nodes in a network by exploring the ability of all act as intermediaries for information exchange. However, one its assumptions, i.e., contributions shortest paths are equal, inconsistent with variations spatial interactions along these and has been questioned when applied networks. Hence, this paper proposes interaction incorporated betweenness (SIBC) SIBC weights path between each node pair according intensity them,...
The efficient isolation and molecular analysis of circulating tumor cells (CTCs) from whole blood at single-cell level are crucial for understanding metastasis developing personalized treatments. viability isolated is the key prerequisite downstream analysis, especially RNA sequencing. This study develops a laser-induced forward transfer -assisted microfiltration system (LIFT-AMFS) high-viability CTC enrichment retrieval blood. LIFT-compatible double-stepped microfilter (DSMF), central to...
A profluorescent probe that has no fluorescent response to H2O2, iron or copper ions but can be readily activated in the presence of both H2O2 and Fe (or Cu) ion been developed; is capable detecting oxidative stress promoted by (i.e. Fenton reaction conditions) living cells.
This work reports an integrated platform combining localized-surface plasmon resonance (LSPR) and microfluidic chips to carry out multiplexed label-free protein analysis. We fabricated optical bench enable detection using only a laboratory UV-Vis spectrophotometer. assay not improves throughput, but also allows quantitative
Multiplexed assay of analytes is great importance for clinical diagnostics and other analytical applications. Barcode-based bioassays with the ability to encode decode may realize this goal in a straightforward consistent manner. We present here microfluidic barcoded chip containing several sets microchannels different widths, imitating commonly used barcode. A single microchip can carry out tens individual protein/nucleic acid assays (encode) immediately yield all results by portable...
Paper-based platforms are ideal for on-site surveillance of infectious diseases in low-resource settings due to their simplicity, self-containment, and low cost. The two most popular materials used paper-based nitrocellulose cellulose. membrane has a high protein binding affinity, but its price is an issue. Cellulose paper inexpensive allows intricate fluidic control more sophisticated biochemical reactions, it affinity. By examining the microstructure cellulose paper, we discover that...
Few-shot image generation is a challenging task since it aims to generate diverse new images for an unseen category with only few images. Existing methods suffer from the trade-off between quality and diversity of generated To tackle this problem, we propose Hyperbolic Attribute Editing (HAE), simple yet effective method. Unlike other that work in Euclidean space, HAE captures hierarchy among using data seen categories hyperbolic space. Given well-trained HAE, can be by moving latent code...