A5004995452- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Invertebrate Immune Response Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Insect symbiosis and bacterial influences
- Erythrocyte Function and Pathophysiology
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Axon Guidance and Neuronal Signaling
- Cellular transport and secretion
- Insect Resistance and Genetics
- 14-3-3 protein interactions
- Biomarkers in Disease Mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Heat shock proteins research
- Neurobiology and Insect Physiology Research
- Reproductive Biology and Fertility
- Ubiquitin and proteasome pathways
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- ATP Synthase and ATPases Research
- Circadian rhythm and melatonin
McGill University
2013-2024
Craig Technologies (United States)
2013-2016
University of Wisconsin–Madison
1999-2003
Institute of Zoology
2000
Marine Biological Laboratory
1999
University of Waterloo
1995-1997
Xenopus oocytes assemble an array of F-actin and myosin 2 around plasma membrane wounds. We analyzed this process in living using confocal time-lapse (four-dimensional) microscopy. Closure wounds requires assembly contraction a classic “contractile ring” composed 2. However, ring works concert with 5–10-μm wide “zone” localized actin assembly. The zone forms before the can be uncoupled from by inhibition cortical flow contractility. contractility contractile are required for stability...
Abstract Many key cellular functions, such as cell motility and differentiation are mediated by Rho‐associated protein kinases (ROCKs). Numerous studies have been conducted to examine the ROCK signal transduction pathways involved in these motile contractile events with aid of pharmacological inhibitors Y‐27632. However molecular mechanism action Y‐27632 has not fully defined. To assess relative contribution Rho effectors effects Y‐27632, we compared cytoskeletal phenotype, wound healing...
Water content within the epididymis of male reproductive system is stringently regulated to promote sperm maturation. Several members aquaporin (AQP) family water channel-forming integral membrane proteins have been identified in epididymal cells, but expression profiling for this epithelium presently incomplete, and no AQP isoform has yet on basolateral plasma membranes these cells. In study, we explored by RT-PCR light microscopy immunolocalizations using peroxidase wide-field fluorescence...
Expression of human Bax, a cardinal regulator mitochondrial membrane permeabilization, causes death in yeast. We screened cDNA library for suppressors Bax-mediated yeast and identified 14-3-3β/α, protein whose paralogs have numerous chaperone-like functions. Here, we show that, cells expressing 14-3-3β/α are able to complement deletion the endogenous 14-3-3 confer resistance variety different stresses including cadmium cycloheximide. The expression also conferred induced by target rapamycin...
The role of the cytoskeleton in regulating mitochondrial distribution dividing mammalian cells is poorly understood. We previously demonstrated that mitochondria are transported to cleavage furrow during cytokinesis a microtubule-dependent manner. However, exact subset spindle microtubules and molecular machinery involved remains unknown. employed quantitative imaging techniques structured illumination microscopy analyse spatial temporal relationship with actin contractile ring HeLa cells....
ABSTRACT Coordinated interplay of the microtubule and actin cytoskeletons has long been known to be crucial for many cellular processes including cell migration cytokinesis. However, interactions between these two systems have difficult document by conventional approaches, a variety technical reasons. Here distribution f-actin microtubules were analyzed in absence fixation using Xenopus egg extracts as an vitro source f-actin, demembranated sperm nucleate asters, fluorescent phalloidin probe...
During embryogenesis, the pancreas develops from separate dorsal and ventral buds, which fuse to form mature pancreas. Little is known about functional differences between these two buds or relative contribution of cells derived each region after fusion. To follow fate bud in fusion, we produced chimeric Elas-GFP transgenic/wild-type embryos either pancreatic expressed GFP. We found that migrate extensively into whereas converse does not occur. Moreover, annular tissue composed exclusively...
Mitochondria are dynamic organelles with multiple cellular functions, including ATP production, calcium buffering, and lipid biosynthesis. Several studies have shown that mitochondrial positioning is regulated by the cytoskeleton during cell division in several eukaryotic systems. However, distribution of mitochondria mammalian cytokinesis whether has not been examined. Using live spinning disk confocal microscopy quantitative analysis fluorescence intensity, we demonstrate recruited to...
Discovered in 1909 by Retzius and described mainly morphology, the cytoplasmic droplet of sperm (renamed here Hermes body) is conserved among all mammalian species but largely undefined at molecular level. Tandem mass spectrometry isolated body from rat epididymal characterized 1511 proteins, 43 which were localized to structure situ light microscopy two quantitative electron localization. Glucose transporter 3 (GLUT-3) glycolytic enzymes, selected membrane traffic cytoskeletal proteins...
Cortical flow, the directed movement of cortical F-actin and organelles, is a basic cellular motility process. Microtubules are thought to somehow direct but whether they do so by stimulating or inhibiting contraction actin cytoskeleton subject debate. Treatment ofXenopus oocytes with phorbol 12-myristate 13-acetate (PMA) triggers flow toward animal pole oocyte; this suppressed microtubules. To determine how suppression occurs it can control direction were subjected localized manipulation...
Genetically programmed cell death (PCD) mechanisms, including apoptosis, are important for the survival of metazoans since it allows, among things, removal damaged cells that interfere with normal function. Cell due to PCD is observed in processes such as aging and a number pathophysiologies hypoxia (common causes heart attacks strokes) subsequent tissue reperfusion. Conversely, loss apoptotic responses associated development tumors. So far, limited success preventing unwanted has been...
Ferritin is a sub-family of iron binding proteins that form multi-subunit nanotype storage structures and prevent oxidative stress induced apoptosis. Here we describe the identification characterization human ferritin, heavy polypeptide 1 (FTH1) as suppressor pro-apoptotic murine Bax sequence in yeast. In addition demonstrate FTH1 general pro-survival since it also prevents cell death inducing effects copper when heterologously expressed Although ferritins are phylogenetically widely...