A5035684616- Image Processing Techniques and Applications
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Photoreceptor and optogenetics research
- Cardiac electrophysiology and arrhythmias
- Iterative Learning Control Systems
- Neurogenesis and neuroplasticity mechanisms
- Hedgehog Signaling Pathway Studies
Montreal Clinical Research Institute
2015
McGill University
2015
Advances in imaging, segmentation and tracking have led to the routine generation of large complex microscopy datasets. New tools are required process this 'phenomics' type data. Here, we present 'Cell PLasticity Analysis Tool' (cellPLATO), a Python-based analysis software designed for measurement classification cell behaviours based on clustering features morphology motility. Used after tracking, tool extracts from each per timepoint, using them segregate cells into dimensionally reduced...
During nervous system development, gradients of Sonic Hedgehog (Shh) and Netrin-1 attract growth cones commissural axons toward the floor plate embryonic spinal cord. Mice defective for either Shh or signaling have axon guidance defects, suggesting that both are required correct guidance. However, how collaborate to guide is not known. We first quantified steepness gradient in cord found it mostly very shallow. then developed an vitro microfluidic assay simulate these shallow gradients....
Abstract Coordinated cardiomyocyte contraction drives the mammalian heart to beat and circulate blood. No consensus model of geometrical arrangement exists, due limited spatial resolution whole imaging methods piecemeal nature studies based on histological sections. By combining microscopy computer vision, we produced first‐ever three‐dimensional orientation reconstruction across mouse ventricular walls at micrometer scale, representing a gain three orders magnitude in resolution. We...
Abstract Stain-free, single-cell segmentation and tracking is tantamount to the holy grail of microscopic cell migration analysis. Phase contrast microscopy (PCM) images with cells at high density are notoriously difficult segment accurately; thus, manual remains de facto standard practice. In this work, we introduce Usiigaci, an all-in-one, semi-automated pipeline segment, track, visualize movement morphological changes in PCM. instance-aware accomplished using a mask regional convolutional...
Abstract The coordinated contraction of myocytes drives the heart to beat and circulate blood. Due limited spatial resolution whole imaging piecemeal nature high-magnification studies, a confirmed model myofiber geometry does not yet exist. Using microscopy computer vision we report first three-dimensional reconstruction myofibers across entire mouse ventricular walls at micron scale, representing gain three orders magnitude in over existing models. Our analysis reveals prominent...