A5071834063- Microtubule and mitosis dynamics
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cellular Mechanics and Interactions
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- 14-3-3 protein interactions
- Cardiomyopathy and Myosin Studies
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Genetic and Kidney Cyst Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Function and Risk Factors
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Protein Structure and Dynamics
- Advanced Fluorescence Microscopy Techniques
- Tissue Engineering and Regenerative Medicine
- Peripheral Neuropathies and Disorders
- Mass Spectrometry Techniques and Applications
- ATP Synthase and ATPases Research
Institute for Stem Cell Biology and Regenerative Medicine
2015-2023
Howard Hughes Medical Institute
2014-2016
University of California, San Francisco
2014-2016
Max Planck Society
2007-2009
Max Planck Institute of Molecular Physiology
2009
Indian Institute of Science Bangalore
2006
Jawaharlal Nehru Centre for Advanced Scientific Research
2006
Septins constitute a group of GTP-binding proteins involved in cytokinesis and other essential cellular functions. They form heterooligomeric complexes that polymerize into nonpolar filaments are dynamic during different stages the cell cycle. Posttranslational modifications interacting partners widely accepted regulators septin filament function, but contribution nucleotide is undefined due to lack detailed structural information. Previous low-resolution structures showed G domain assembles...
Objective To determine the cause and course of a novel syndrome with progressive encephalopathy brain atrophy in children. Methods Clinical whole-exome sequencing was performed for global developmental delay intellectual disability; some patients also had spastic paraparesis evidence clinical regression. Six were identified de novo missense mutations kinesin gene KIF1A. The predicted functional disruption these assessed silico to compare calculated conformational flexibility estimated...
Resource22 June 2020Open Access Source DataTransparent process Structural insights into actin filament recognition by commonly used cellular markers Archana Kumari Center for Cardiovascular Biology and Diseases, Institute Stem Cell Science Regenerative Medicine, Bengaluru, India Search more papers this author Shubham Kesarwani Manipal Academy of Higher Education, Manipal, Manjunath G Javoor Kutti R Vinothkumar Corresponding Author [email protected] National Biological Sciences, TIFR,...
Calculations predict that cation−π interactions make an important contribution to protein stability. While there have been some attempts experimentally measure strengths of using peptide model systems, much less experimental data are available for globular proteins. We attempted determine the magnitude Lys with aromatic amino acids in four different proteins (LIVBP, MBP, RBP, and Trx). In each case, was replaced Gln Met. a separate series experiments, acid pair by Leu. Stabilities wild-type...
Abstract The amino acid Pro is more rigid than other naturally occurring acids and, in proteins, lacks an amide hydrogen. To understand the structural and thermodynamic effects of substitutions, it was introduced at 13 different positions four leucine–isoleucine–valine binding protein, maltose ribose thioredoxin. Three protein mutants were characterized by X‐ray crystallography to confirm that no changes had occurred upon mutation. In remaining cases, fluorescence CD spectroscopy used show...
Microtubule cytoskeleton exists in various biochemical forms different cells due to tubulin posttranslational modifications (PTMs). Tubulin PTMs are known affect microtubule stability, dynamics, and interaction with MAPs motors a specific manner, widely as code hypothesis. At present, there no tool that can specifically mark living cells, thus severely limiting our understanding of their dynamics cellular functions. Using yeast display library, we identified binder against terminal tyrosine...
The emergence of new escape mutants the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has escalated its penetration among human population and reinstated status as a global pandemic. Therefore, developing effective antiviral therapy against emerging SARS-CoV variants other viruses in short period becomes essential. Blocking SARS-CoV-2 entry into host cells by disrupting spike glycoprotein-angiotensin-converting enzyme interaction already been exploited for vaccine development...
ABSTRACT Post-translational modifications (PTMs) of α/β-tubulin are believed to regulate interactions with microtubule binding proteins. A well-characterized PTM involves the removal and re-ligation C-terminal tyrosine on α-tubulin, but purpose this tyrosination-detyrosination cycle remains elusive. Here, we examined processive motility mammalian dynein complexed dynactin BicD2 (DDB) tyrosinated versus detyrosinated microtubules. Motility was decreased ~4-fold microtubules, constituting...
Abstract Cellular studies of filamentous actin (F-actin) processes commonly utilize fluorescent versions toxins, peptides and proteins that bind actin. While the choice these markers has been largely based on availability ease, there is a severe dearth structural data for an informed judgment in employing suitable F-actin particular requirement. Here we describe electron cryomicroscopy structures phalloidin, lifeAct utrophin bound to F-actin, providing first high-resolution comparison widely...
Abstract Coordinated cardiomyocyte contraction drives the mammalian heart to beat and circulate blood. No consensus model of geometrical arrangement exists, due limited spatial resolution whole imaging methods piecemeal nature studies based on histological sections. By combining microscopy computer vision, we produced first‐ever three‐dimensional orientation reconstruction across mouse ventricular walls at micrometer scale, representing a gain three orders magnitude in resolution. We...