A5000277363- Bacteriophages and microbial interactions
- HIV Research and Treatment
- Crystallization and Solubility Studies
- Vanadium and Halogenation Chemistry
- Vibrio bacteria research studies
- X-ray Diffraction in Crystallography
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- Organometallic Complex Synthesis and Catalysis
- Receptor Mechanisms and Signaling
- Viral gastroenteritis research and epidemiology
- Oxidative Organic Chemistry Reactions
- Tuberculosis Research and Epidemiology
- Protein Structure and Dynamics
- 3D Shape Modeling and Analysis
- Microbial Natural Products and Biosynthesis
- Influenza Virus Research Studies
- Robotics and Sensor-Based Localization
- Clostridium difficile and Clostridium perfringens research
- Polyoxometalates: Synthesis and Applications
- Enzyme Structure and Function
- Metal-Catalyzed Oxygenation Mechanisms
Indian Institute of Science Bangalore
2016-2025
Istituto di Scienza e Tecnologie dell'Informazione "Alessandro Faedo"
2023
National Research Council
2023
GTx (United States)
2022
Technische Universität Dresden
2022
University of Michigan
2011-2019
Indian Academy of Sciences
2019
Indian Institute of Technology Kharagpur
2013-2015
Michigan United
2015
National Institute of Cholera and Enteric Diseases
2009-2013
Flaviviruses, the human pathogens responsible for dengue fever, West Nile tick-borne encephalitis, and yellow are endemic in tropical temperate parts of world. The flavivirus nonstructural protein 1 (NS1) functions genome replication as an intracellular dimer immune system evasion a secreted hexamer. We report crystal structures full-length, glycosylated NS1 from viruses. hexamer is similar to solution visualized by single-particle electron microscopy. Recombinant binds lipid bilayers...
The active-state complex between an agonist-bound receptor and a guanine nucleotide-free G protein represents the fundamental signaling assembly for majority of hormone neurotransmitter signaling. We applied single-particle electron microscopy (EM) analysis to examine architecture agonist-occupied β 2 -adrenoceptor (β AR) in with heterotrimeric Gs (Gαsβγ). EM 2D averages 3D reconstructions detergent-solubilized reveal overall that is very good agreement crystal structure ternary complex....
Yeast VPS13 is the founding member of a eukaryotic gene family growing interest in cell biology and medicine. Mutations three four human genes cause autosomal recessive neurodegenerative or neurodevelopmental disease, making yeast Vps13p an important structural functional model. Using cell-free reconstitution with purified Vps13p, we show that directly required both for transport from trans-Golgi network (TGN) to late endosome/prevacuolar compartment (PVC) TGN homotypic fusion. must be...
Nipah virus (NiV) is a paramyxovirus that infects host cells through the coordinated efforts of two envelope glycoproteins. The G glycoprotein attaches to cell receptors, triggering fusion (F) execute membrane fusion. Here we report first crystal structure pre-fusion form NiV-F ectodomain. Interestingly this also revealed hexamer-of-trimers encircling central axis. Electron tomography virus-like particles supported hexameric model, and biochemical analyses F assembly in solution....
Desensitization, signaling, and trafficking of G-protein-coupled receptors (GPCRs) are critically regulated by multifunctional adaptor proteins, β-arrestins (βarrs). The two isoforms βarrs (βarr1 2) share a high degree sequence structural similarity; still, however, they often mediate distinct functional outcomes in the context GPCR signaling regulation. A mechanistic basis for such divergence βarr is still lacking. By using set complementary approaches, including antibody-fragment-based...
Virtually all SARS-CoV-2 vaccines currently in clinical testing are stored a refrigerated or frozen state prior to use. This is major impediment deployment resource-poor settings. Furthermore, several of them use viral vectors mRNA. In contrast protein subunit vaccines, there limited manufacturing expertise for these nucleic-acid-based modalities, especially the developing world. Neutralizing antibodies, clearest known correlate protection against SARS-CoV-2, primarily directed...
The receptor binding domain (RBD) of SARS-CoV-2 is the primary target neutralizing antibodies. We designed a trimeric, highly thermotolerant glycan engineered RBD by fusion to heterologous, poorly immunogenic disulfide linked trimerization derived from cartilage matrix protein. protein expressed at yield ∼80-100 mg/L in transiently transfected Expi293 cells, as well CHO and HEK293 stable cell lines formed homogeneous disulfide-linked trimers. When lyophilized, these possessed remarkable...
ABSTRACT The henipaviruses, Hendra virus (HeV) and Nipah (NiV), are paramyxoviruses discovered in the mid- to late 1990s that possess a broad host tropism known cause severe often fatal disease both humans animals. HeV NiV infect cells by pH-independent membrane fusion mechanism facilitated their attachment (G) (F) glycoproteins. Here, several soluble forms of henipavirus F (sF) were engineered characterized. Recombinant sF was produced deleting transmembrane (TM) cytoplasmic tail (CT)...
Broadly neutralizing antibodies (bNAbs) have demonstrated protective effects against HIV-1 in primate studies and recent human clinical trials. Elite neutralizers are potential candidates for isolation of bNAbs. The coexistence bNAbs such as BG18 with neutralization-susceptible autologous viruses an HIV-1-infected adult elite controller has been suggested to control viremia. Disease progression is faster children than adults. Plasma multiple epitope specificities developed chronically...
Rapid emergence of the SARS-CoV-2 variants has dampened protective efficacy existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine immunogenicity by presenting multiple copies desired antigens in repetitive manner which closely mimics natural infection. We have applied nanoparticle display combined with SpyTag–SpyCatcher system design encapsulin–mRBD, displaying 180 monomeric spike receptor-binding domain (RBD). Here we show that encapsulin–mRBD is strongly...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of mononuclear and binuclear oxidic vanadium(V), VVVV, VVVIV azophenolatesSomnath Dutta, Partha Basu, Animesh ChakravortyCite this: Inorg. Chem. 1993, 32, 23, 5343–5348Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/ic00075a063https://doi.org/10.1021/ic00075a063research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMononuclear manganese(IV) in tridentate ONO coordination. Synthesis, structure, and redox regulation via oxygen donor variationSomnath Dutta, Partha Basu, Animesh ChakravortyCite this: Inorg. Chem. 1991, 30, 21, 4031–4037Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://pubs.acs.org/doi/10.1021/ic00021a012https://doi.org/10.1021/ic00021a012research-articleACS...
Zoonotic spillover of sarbecoviruses to humans resulted in theSARS-CoV-1 outbreak 2003and the current COVID-19 pandemic caused by SARS-CoV-2. In both cases, viral spikeprotein (S) is principal target neutralising antibodies that prevent infection. Within spike, immunodominant receptor-binding domain (RBD) primary convalescent sera and vaccine recipients. We have constructed stabilized RBD derivatives different sarbecoviruses: SARS-CoV-1 (Clade 1a), WIV-1 RaTG13 1b), RmYN02 2) BtKY72 3)....
<title>Abstract</title> The infectious microbe <italic>Staphylococcus aureus</italic> releases an array of cytotoxic pore-forming toxins (PFTs) that severely damage the cell membrane during bacterial infection. However, interaction interfaces between host and toxin were hardly explored. So far, there are no pore, intermediate structures these available in presence bio-membrane, which could elucidate mechanism. Therefore, we investigated structure different conformational states this...
RND efflux pumps are a major cause of multidrug resistance in bacteria, particularly Gram-negative bacteria. They complex molecular machines forming tripartite assemblies that actively pump out wide range antimicrobial agents, including antibiotics, biocides and host defence molecules. However, the presence multiple with overlapping functions single bacterium raises questions about their individual functional relevance. In this study, we determined Cryo-EM structures two distinct HAE-RND...
The envelope (Env) glycoprotein from viruses circulating in elite–neutralizers individuals who naturally develop broadly neutralizing antibody (bNAb) responses, serves as a potential template for rational HIV–1 vaccine design. Here, we engineered soluble clade–C Env trimer (330) derived pediatric elite–neutralizer (AIIMS_330) using SOSIP, NFL, and self–assembling ferritin–nanoparticles (NP) platforms. Both SOSIP NP–displayed Envs exhibited high thermostability nanomolar binding affinity to...