A5031605065- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- COVID-19 epidemiological studies
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Biosensors and Analytical Detection
- Vector-Borne Animal Diseases
- interferon and immune responses
- Immunotherapy and Immune Responses
- Viral Infections and Outbreaks Research
- Diverse Scientific Research Studies
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Phytochemicals and Medicinal Plants
- Vitamin C and Antioxidants Research
- Pharmacological Effects of Natural Compounds
- Virology and Viral Diseases
- Computational Drug Discovery Methods
- Heat shock proteins research
National University of Singapore
2025
Translational Health Science and Technology Institute
2020-2025
Panacea Biotec (India)
2025
Duke-NUS Medical School
2018-2023
Government of Haryana
2023
Government of India
2021-2022
International Centre for Genetic Engineering and Biotechnology
2015
International Centre for Genetic Engineering and Biotechnology
2013
Cross-species transmission of viruses from wildlife animal reservoirs poses a marked threat to human and health
SARS-CoV-2 variants of concern (VOCs) have threatened COVID-19 vaccine effectiveness. We aimed to assess the effectiveness ChAdOx1 nCoV-19 vaccine, predominantly against delta (B.1.617.2) variant, in addition cellular immune response vaccination.
There is a desperate need for safe and effective vaccines, therapies, diagnostics SARS- coronavirus 2 (CoV-2), the development of which will be aided by discovery potent selective antibodies against relevant viral epitopes. Human phage display technology has revolutionized process identifying optimizing antibodies, providing facile entry points further applications. Herein, we use this to search targeting receptor-binding domain (RBD) CoV-2. Specifically, screened naïve human semisynthetic...
Virtually all SARS-CoV-2 vaccines currently in clinical testing are stored a refrigerated or frozen state prior to use. This is major impediment deployment resource-poor settings. Furthermore, several of them use viral vectors mRNA. In contrast protein subunit vaccines, there limited manufacturing expertise for these nucleic-acid-based modalities, especially the developing world. Neutralizing antibodies, clearest known correlate protection against SARS-CoV-2, primarily directed...
Cytokine release syndrome (CRS) due to severe acute respiratory coronavirus-2 (SARS-CoV-2) infection leads life-threatening pneumonia which has been associated with coronavirus disease (COVID-19) pathologies. Centuries-old Asian traditional medicines such as Withania somnifera (L.) Dunal (WS) and Tinospora cordifolia (Willd.) Miers (TC) possess potent immunomodulatory effects were used by the AYUSH ministry, in India during COVID-19 pandemic. In present study, we investigated WS TC's...
Dengue is a mosquito-borne viral disease with global prevalence. It caused by four closely-related dengue viruses (DENVs 1–4). A vaccine that can protect against all an unmet public health need. Live attenuated development efforts have encountered unexpected interactions between the viruses, raising safety concerns. This has emphasized need to explore non-replicating options. Virus-like particles (VLPs) which elicit robust immunity in absence of infection offer potential promise for...
The receptor binding domain (RBD) of SARS-CoV-2 is the primary target neutralizing antibodies. We designed a trimeric, highly thermotolerant glycan engineered RBD by fusion to heterologous, poorly immunogenic disulfide linked trimerization derived from cartilage matrix protein. protein expressed at yield ∼80-100 mg/L in transiently transfected Expi293 cells, as well CHO and HEK293 stable cell lines formed homogeneous disulfide-linked trimers. When lyophilized, these possessed remarkable...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a positive-strand RNA virus. The viral genome capped at the 5' end, followed by an untranslated region (UTR). There poly(A) tail 3' preceded UTR. self-interaction between regulatory elements present within and UTRs their interaction with host/virus-encoded proteins mediate function of UTRs. Using RNA-protein detection (RaPID) assay coupled to liquid chromatography tandem mass spectrometry, we identified host partners SARS-CoV-2...
Dengue poses a serious public health risk to nearly half the global population. It causes ~400 million infections annually and is considered be one of fastest spreading vector-borne diseases. Four distinct serotypes dengue viruses (DENV-1, -2, -3 -4) cause disease, which may either mild or extremely severe. Antibody-dependent enhancement (ADE), by pre-existing cross-reactive antibodies, major mechanism underlying severe disease. This mandates that preventive vaccine must confer simultaneous...
The newly emerged novel coronavirus, SARS-CoV-2, the causative agent of COVID-19 has proven to be a threat human race globally, thus, vaccine development against SARS-CoV-2 is an unmet need driving mass vaccination efforts. receptor binding domain spike protein this coronavirus multiple neutralizing epitopes and associated with viral entry. Here we have designed characterized fragment 330-526 as (RBD ) two native glycosylation sites (N331 N343); potential subunit candidate. We initially RBD...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infection initiates with viral entry in the upper tract, leading to disease 2019 (COVID-19). COVID-19 is characterized by pulmonary pathologies associated failure. Thus, therapeutics aimed at inhibiting of virus or its internalization tract are interest. Herein, we report prophylactic application two intranasal formulations provided National Medicinal Plant Board (NMPB), Anu oil and til tailya, hamster model SARS-CoV-2 infection....
Severe coronavirus disease (COVID-19) is accompanied by acute respiratory distress syndrome and pulmonary pathology, presented mostly with an inflammatory cytokine release, a dysregulated immune response, skewed neutrophil/lymphocyte ratio, hypercoagulable state. Though vaccinations have proved effective in reducing the COVID-19-related mortality, limitation of use vaccine against immunocompromised individuals, those comorbidity, emerging variants remains concern. In current study, we...
Crimean-Congo hemorrhagic fever is a tick-borne zoonotic disease that may be severe and present in many African countries. We aimed to understand the seroprevalence risk for virus Tanzania by testing archived serum samples from patients enrolled prospective cohort study.
Rapid development of coronavirus disease 2019 (COVID-19) vaccines and antiviral drugs have significantly reduced morbidity mortality worldwide. Although most the were developed initially with ancestral Wuhan antigen, here, we report immunological efficacy a whole-virion-inactivated vaccine candidate (CoviWall) to combat deadly B.1.617.2 (Delta strain) infection. In current study, demonstrate consistent manufacturing process under Good Manufacturing Practice for CoviWall its characterization...
Apart from the canonical fingers, palm and thumb domains, RNA dependent polymerases (RdRp) viral order Nidovirales possess two additional domains. Of these, function of Nidovirus RdRp associated nucleotidyl transferase domain (NiRAN) remains unanswered. The elucidation 3D structure severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), provided first ever insights into organisation possible functional characteristics NiRAN domain. Using in silico tools, we predict that assumes a...
Dromedary camels are important reservoir hosts of various coronaviruses, including Middle East respiratory syndrome coronavirus (MERS-CoV) that cause human infections. CoV genomes regularly undergo recombination during infection as observed in bat SARS-related CoVs. Here we report for the first time only a small proportion MERS-CoV receptor-binding domain positive (RBD) spike protein camel sera Kenya were also seropositive to nucleocapsid (NP). In contrast, many them contain antibodies...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) initiates infection by attachment of the surface-exposed spike glycoprotein to host cell receptors. The (S) is a promising target for inducing immune responses and providing protection; thus ongoing efforts SARS-CoV-2 vaccine therapeutic developments are mostly spiraling around S glycoprotein. matured functional presented on virion surface as trimers, which contain two subunits, such S1 (virus attachment) S2 fusion). subunit...
To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected 2005-2013. After initial screen with luciferase immunoprecipitation system and secondary confirmation virus neutralization test, identified at least seven specific antibodies against PRV both assays. Our findings confirm that spillover into human populations is relatively common this region of the world.
Although efficacious vaccines have significantly reduced the morbidity and mortality of COVID-19, there remains an unmet medical need for treatment options, which monoclonal antibodies (mAbs) can potentially fill. This is exacerbated by emergence spread SARS-CoV-2 variants concern (VOCs) that shown some resistance to vaccine responses. Here we report isolation five neutralizing mAbs from Indian convalescent donor, out two (THSC20.HVTR04 THSC20.HVTR26) showed potent neutralization VOCs at...