A5028602851- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Parkinson's Disease Mechanisms and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer Treatment and Pharmacology
- ATP Synthase and ATPases Research
- Electrochemical sensors and biosensors
- Ubiquitin and proteasome pathways
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Nerve injury and regeneration
- Multiple Myeloma Research and Treatments
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Hereditary Neurological Disorders
- Tactile and Sensory Interactions
- Cell death mechanisms and regulation
- Cellular Mechanics and Interactions
- Epigenetics and DNA Methylation
- Pain Mechanisms and Treatments
- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
- Neuropeptides and Animal Physiology
- Eicosanoids and Hypertension Pharmacology
Université de Limoges
2021-2025
Inserm
2010-2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016-2022
National Institutes of Health
2017-2022
Medical University of South Carolina
2019-2022
Université de Bordeaux
2021
Mayo Clinic in Arizona
2014-2019
Aix-Marseille Université
2010-2014
Centre de Recherche en Cancérologie de Marseille
2013
Mitochondrial membrane potential (ΔΨm) is a global indicator of mitochondrial function. Previous reports on heterogeneity ΔΨm were qualitative or semiquantitative. Here, we quantified intercellular differences in unsynchronized human cancer cells, cells synchronized G1, S, and G2, fibroblasts. We assessed using two-pronged microscopy approach to measure relative fluorescence tetramethylrhodamine methyl ester (TMRM) absolute values ΔΨm. showed that more heterogeneous compared fibroblasts, it...
Peripheral myelination disorders encompass a variety of that affect myelin sheaths in the peripheral nervous system. The Charcot-Marie-Tooth disease (CMT), most common inherited neuropathy, is one prevalent among them. CMT stems from wide range genetic causes disrupt nerve conduction, leading to progressive muscle weakness and atrophy, sensory loss, motor function impairment. Historically, study these has relied heavily on animal studies, owing challenges accessing human cells. However,...
Statins, widely used to treat hypercholesterolemia, inhibit the 3-hydroxy-3-methylglutaryl-coenzyme A reductase, rate-limiting enzyme of de novo cholesterol (Chol) synthesis. Statins have been also reported slow tumor progression. In cancer cells, ATP is generated both by glycolysis and oxidative phosphorylation. Mitochondrial membrane potential (ΔΨ), a readout mitochondrial metabolism, sustained oxidation respiratory substrates in Krebs cycle generate NADH flavin adenine dinucleotide, which...
Bcl-2 is commonly overexpressed in tumors, where it often associated with unfavorable outcome. However, has also been linked to a favorable sensitivity microtubule-targeting agents (MTAs). We show that Bcl-2– overexpressing lung and breast cancer cells were more sensitive both paclitaxel vinorelbine. overexpression significantly potentiated vivo efficacy of paclitaxel, terms tumor volume decrease survival benefits, models nude mice bearing xenografts. To further investigate this effect...
// Marion Le Grand 1,2 , Amandine Rovini 1,3 Veronique Bourgarel-Rey Stephane Honore Sonia Bastonero 1 Diane Braguer and Manon Carre Aix Marseille Université, Inserm, CRO2 UMR_S 911, Marseille, France 2 APHM, Hopital Timone, 3 Department of Neurology, Mayo Clinic, Rochester, MN, USA. Correspondence: Carré, email: Keywords : EB1 phosphorylation, microtubule dynamics, mitochondrial ROS, chemotherapy, Akt/GSK3β pathway Received March 6, 2014 Accepted May 16, Published 18, Abstract...
It is well established that α-synuclein (α-syn) binding from solution to the surface of membranes composed negatively charged and/or non-lamellar lipids can be characterized by equilibrium dissociation constants tens micromolar. Previously, we have found a naturally occurring nanopore mitochondrial voltage-dependent anion channel (VDAC), reconstituted into planar bilayers plant-derived lipid, responds α-syn at nanomolar concentrations. Here, using lipid mixtures mimic composition outer...
We previously showed that vinflunine, a microtubule-targeting drug of the Vinca-alkaloid family exerted its anti-angiogenic/anti-migratory activities through an increase in microtubule dynamics and inhibition targeting to adhesion sites. Such effect was associated with reduction EB1 comet length at (+) ends. In this work we first pro-angiogenic vascular endothelial growth factor VEGF suppressed living Human Umbilical Vein Endothelial Cells (HUVECs), increased by 40%, induced bind all along...
Tubulin is a well-established target of microtubule-targeting agents (MTAs), widely used class chemotherapeutic drugs. Yet, aside from their powerful anti-cancer efficiency, MTAs induce dose-limiting and debilitating peripheral neurotoxicity. Despite intensive efforts in the development neuroprotective agents, there are currently no approved therapies to effectively manage chemotherapy-induced neuropathy (CIPN). Over last decade, attempts unravel pathomechanisms underlying CIPN led...
We propose in this article to compare the efficiency of two chemotherapeutic schedules: traditional and metronomic. For this, we develop a new mathematical model describing growth dynamics tumor endothelial cells as well impact molecules oxygen or vascular factor on dynamics. The construction: biological assumptions, description equations their discretization, constitutes core article. Numerical experiments illustrate expected behavior disease under schedules.
Sensory neurons of the peripheral nervous system are critical in health and disease. derived from induced pluripotent stem (iPS) cells now being used increasingly for vitro models neuropathy, pain, neurotoxicity. DNA methylation is neurodevelopment has been implicated many neuronal diseases, but not examined iPS-derived sensory neurons. In order to better characterize neuron model, we have undertaken a genome-wide study on human iPS during differentiation through reduced representation...
Metabolic flexibility is the ability of a cell to adapt its metabolism changes in surrounding environment. Such adaptability, combined with apoptosis resistance provides cancer cells survival advantage. Mitochondrial voltage-dependent anion channel 1 (VDAC1) has been defined as metabolic checkpoint at crossroad these two processes. Here, we show that hypoxia-induced cleaved form VDAC1 (VDAC1-ΔC) implicated both up-regulation glycolysis and mitochondrial respiration. We demonstrate VDAC1-ΔC,...
Human-adipose-tissue-derived mesenchymal stromal cells (AD-MSCs) are currently being tested as autologous-cell-based therapies for use in tissue healing and regeneration. Recent studies have also demonstrated that AD-MSC-derived exosomes contribute to repair peripheral nerve Subcutaneous abdominal adipose (AAT) is divided into two layers: the superficial layer (sAAT) deep (dAAT). However, it unclear whether there particular characteristics of each terms AD-MSC regenerative potential. Using...