A5020194060- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Synthesis and Reactions of Organic Compounds
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Analytical Chemistry and Sensors
- Cancer Genomics and Diagnostics
- Cancer, Stress, Anesthesia, and Immune Response
- Advanced Chemical Sensor Technologies
- Cyclization and Aryne Chemistry
- Cell death mechanisms and regulation
- X-ray Diffraction in Crystallography
- Chemical synthesis and alkaloids
- Crystallization and Solubility Studies
- Asymmetric Synthesis and Catalysis
- Monoclonal and Polyclonal Antibodies Research
- Cancer Treatment and Pharmacology
- Click Chemistry and Applications
- Luminescence and Fluorescent Materials
- Neuroblastoma Research and Treatments
- Glycosylation and Glycoproteins Research
- Synthesis and Biological Activity
- RNA Interference and Gene Delivery
Centre de Recherche en Cancérologie de Marseille
2014-2024
Centre National de la Recherche Scientifique
2001-2024
Inserm
2014-2024
Aix-Marseille Université
2014-2024
Centre Camille Jullian
2024
Hôpital de la Timone
2023
Institut Paoli-Calmettes
2018
Institut Pprime
2018
Institut de Neurobiologie de la Méditerranée
2002-2009
Université de Lorraine
1993-2008
We have previously reported that anti-tubulin agents induce the release of cytochrome c from isolated mitochondria. In this study, we show tubulin is present in mitochondria different human cancerous and non-cancerous cell lines. The absence polymerized microtubules cytosolic proteins was checked to ensure an inherent component addition, a salt wash did not By using electron microscopy, then showed localized mitochondrial membranes. As compared with cellular tubulin, enriched acetylated...
Eddy Pasquier 1,5 , Joseph Ciccolini 2 Manon Carre 3 Sarah Giacometti Raphaelle Fanciullino Charlotte Pouchy 1,‡ Marie-Pierre Montero Cindy Serdjebi Maria Kavallaris 1 and Nicolas André 3,4,5 Children's Cancer Institute Australia, Lowy Research Centre, UNSW, Randwick, NSW, Australia Pharmacokinetics Unit, UMR-MD3, Aix-Marseille Univ, Marseille, France INSERM UMR 911, Centre de Recherche en Oncologie biologique et Oncopharmacologie, 4 Hematology & Pediatric Oncology Department, La Timone...
The use of β-blockers for the management hypertension has been recently associated with significant clinical benefits in cancer patients. Herein, we investigated whether could be used combination chemotherapy treatment neuroblastoma.Seven were tested their antiproliferative and anti-angiogenic properties alone, vitro; most potent drug combinations evaluated vivo TH-MYCN mouse model neuroblastoma.Three (i.e., carvedilol, nebivolol propranolol) exhibited anticancer vitro interacted...
Cancer is a leading cause of death and major health problem worldwide. While many effective anticancer agents are available, most drugs currently on the market not specific, raising issues like common side effects chemotherapy. However, recent research hold promises for development more efficient safer drugs. Quinoxaline its derivatives becoming recognized as novel class chemotherapeutic with activity against different tumors. The present review compiles discusses studies concerning...
Abstract Angiogenesis is a critical event in tumor growth and metastasis, which can be inhibited by conventional anticancer drugs such as the microtubule-damaging agent paclitaxel (Taxol). In this study, we investigate mechanism of action on human endothelial cells. We characterize two distinct effects umbilical vein cell microvascular cell-1 proliferation according to drug concentration: cytostatic effect at low concentrations cytotoxic 10 nmol/L. The involves signaling pathways similar...
We previously reported that paclitaxel acted directly on mitochondria isolated from human neuroblastoma SK‐N‐SH cells. Here, we demonstrate the direct mitochondrial effect of observed in vitro is relevant intact After a 2 h incubation with 1 μM paclitaxel, were less condensed. Paclitaxel (1 μM, 1–4 h) also induced 20% increase respiration rate and caspase‐independent production reactive oxygen species by mitochondria. The paclitaxel‐induced release cytochrome c was detected only after 24...
// María Rico 1, 2, * , Baglioni Maryna Bondarenko 3 Nahuel Cesatti Laluce 1 Viviana Rozados Nicolas André 3, 4, 5 Manon Carré O. Graciela Scharovsky Mauricio Menacho Márquez 2 Instituto de Genética Experimental, Facultad Ciencias Médicas, Universidad Nacional Rosario, Argentina El Consejo Investigaciones Científicas y Técnicas, Aix-Marseille Université, Inserm UMR_S 911, Centre Recherche en Oncologie biologique et...
The synthesis of different 4-arylcoumarin analogues combretastatin A-4 led to the identification two new compounds (1 and 2) with potent cytotoxic activity on a CEM leukemia cell line third one completely inactive (compound 3). It was suggested that cytotoxicity 1 2 may be related their interaction microtubules tubulin, since these inhibit microtubule formation from purified tubulin in vitro [Bailly et al. (2003) J. Med. Chem. 46 (25), 5437−5444]. In present study, identified as main target...
// Emilie Denicolaï 1, * , Nathalie Baeza-Kallee Aurélie Tchoghandjian 1 Manon Carré Carole Colin Carine Jiguet Jiglaire Sandy Mercurio Christophe Beclin 2 Dominique Figarella-Branger 3 Inserm, Aix-Marseille Université, CRO2 UMR_S 911, Marseille, 13385, France CNRS, IBDML, Aix Marseille 6216, 13288, APHM, Hôpital de la Timone, Service d'Anatomie Pathologique et Neuropathologie, These two authors have contributed equally to this study Correspondence to: Figarella-Branger, e-mail:...
Abstract Background HIV infection and progression to AIDS is characterized by the depletion of T cells, which could be due, in part, apoptosis mediated extra-cellular HIV-encoded Tat protein as a consequence binding tubulin. Microtubules are tubulin polymers that essential for cell structure division. Molecules target microtubules induce potent anti-cancer drugs. We studied effect on polymerization three variants: HxB2 Eli from patients who rapid progressors (RP) Oyi highly exposed but...
Bcl-2 is commonly overexpressed in tumors, where it often associated with unfavorable outcome. However, has also been linked to a favorable sensitivity microtubule-targeting agents (MTAs). We show that Bcl-2– overexpressing lung and breast cancer cells were more sensitive both paclitaxel vinorelbine. overexpression significantly potentiated vivo efficacy of paclitaxel, terms tumor volume decrease survival benefits, models nude mice bearing xenografts. To further investigate this effect...
// Marion Le Grand 1,2 , Amandine Rovini 1,3 Veronique Bourgarel-Rey Stephane Honore Sonia Bastonero 1 Diane Braguer and Manon Carre Aix Marseille Université, Inserm, CRO2 UMR_S 911, Marseille, France 2 APHM, Hopital Timone, 3 Department of Neurology, Mayo Clinic, Rochester, MN, USA. Correspondence: Carré, email: Keywords : EB1 phosphorylation, microtubule dynamics, mitochondrial ROS, chemotherapy, Akt/GSK3β pathway Received March 6, 2014 Accepted May 16, Published 18, Abstract...
Despite recent advances in deciphering cancer drug resistance mechanisms, relapse is a widely observed phenomenon advanced cancers, mainly due to intratumor clonal heterogeneity. How tumor clones progress and impact each other remains elusive. In this study, we developed 2D 3D non-small cell lung co-culture systems defined phenomenological mathematical model better understand clone dynamics. Our results demonstrated that the drug-sensitive inhibit proliferation of drug-resistant ones under...
Hydrophobically modified pectin derivatives were prepared by immobilization of long alkyl chains (C12−C18) at various substitution ratios, using two different synthetic pathways, one affording covalent fixation and the other leading to a mere ionic association. These display an associative tendency in semidilute as well dilute aqueous solutions. This phenomenon, which stems from both intra- intermolecular interactions between hydrophobic groups, results formation microdomains. The latter can...
Vinflunine, the newest fluorinated Vinca alkaloid, currently in phase III clinical trials, targets microtubule network to induce mitotic block and apoptosis by mechanisms that remain unclear. In current study, we investigated apoptotic pathways induced a wide range of vinflunine concentrations SK-N-SH neuroblastoma cells. The inhibited 50 70% cell growth (IC(50) IC(70)) high extents but failed depolymerize cells G(2)/M. It is interesting IC(50) IC(70) suppressed dynamics, slowed down...
Purpose: Nanoparticles are of rising interest in cancer research, but vitro canonical cell monolayer models not suitable to evaluate their efficacy when prototyping candidates. Here, we developed three-dimensional (3D) spheroid test the trastuzumab-docetaxel immunoliposomes breast prior further testing them vivo. Materials and methods: Immunoliposomes were synthesized using standard thin film method maleimide linker. Two human lines varying Her2 expression tested: Her2+ cells derived from...