A5032329736- Chemical Synthesis and Analysis
- Proteoglycans and glycosaminoglycans research
- Biochemical and Structural Characterization
- Protein Structure and Dynamics
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory Bowel Disease
- Gut microbiota and health
- Glycosylation and Glycoproteins Research
- Mosquito-borne diseases and control
- Pharmacological Effects of Natural Compounds
- Collagen: Extraction and Characterization
- Virology and Viral Diseases
- ATP Synthase and ATPases Research
- Heat shock proteins research
- Cytomegalovirus and herpesvirus research
- Vector-borne infectious diseases
- Invertebrate Immune Response Mechanisms
- HIV/AIDS drug development and treatment
- Helicobacter pylori-related gastroenterology studies
- Carbohydrate Chemistry and Synthesis
- Microscopic Colitis
Sorbonne Université
2009-2024
Centre National de la Recherche Scientifique
2009-2024
Laboratoire de Chimie
2024
Université de Technologie de Compiègne
2024
Laboratoire des Biomolécules
2009-2023
École Normale Supérieure - PSL
2015-2023
Université Paris Sciences et Lettres
2015-2023
Sorbonne University Abu Dhabi
2022
Sorbonne Paris Cité
2022
University of Oslo
2014
Crohn's disease (CD)-associated dysbiosis is characterised by a loss of Faecalibacterium prausnitzii, whose culture supernatant exerts an anti-inflammatory effect both in vitro and vivo. However, the chemical nature compounds has not yet been determined.Peptidomic analysis using mass spectrometry was applied to F. prausnitzii supernatant. Anti-inflammatory effects identified peptides were tested directly on intestinal epithelial cell lines transfected with plasmid construction coding for...
Many intrinsically disordered proteins (IDPs) and protein regions (IDRs) engage in transient, yet specific, interactions with a variety of partners. Often, if not always, partner lead to partial folding the IDR. Characterizing conformational space such complexes is challenging: solution-state NMR, signals IDR interacting region become broad, weak, often invisible, while X-ray crystallography only provides information on fully ordered regions. There thus need for simple method characterize...
Abstract Borrelia , spirochetes transmitted by ticks, are the etiological agents of numerous multisystemic diseases, such as Lyme borreliosis (LB) and tick-borne relapsing fever (TBRF). This study focuses on two surface proteins from subspecies involved in these diseases: CspZ, expressed burgdorferi sensu stricto (also named BbCRASP-2 for complement regulator-acquiring protein 2), factor H binding A (FhbA), hermsii. Numerous including latter, able to evade immune defenses a variety potential...
Numerous collagen mimetic peptides (CMPs) have been engineered using proline derivatives substituted at their C(3) and/or C(4) position in order to stabilize or functionalize triple-helix mimics. However, no example has reported so far with C(5) substitutions. Here, we introduce a fluorinated CMP incorporating trifluoromethyl groups the of pseudoproline residues. In tripeptide models, our CD, NMR, and molecular dynamics (MD) studies shown that, when properly arranged, these residues meet...
The ability of human immunodeficiency virus type 1 (HIV-1) to egress from cells by budding with the cell membrane remains a complex phenomenon unclear steps. HIV-1 viral protein R (Vpr) incorporation in sorting virions relies greatly on interaction group-specific antigen (Gag) C-terminal region, which encompasses p6. complete role p6 is still undetermined; however, it thought that interacts core elements endosomal ESCRT-1, known sort ubiquitinated cargo into multivesicular bodies (MVB)....
The molecular mechanism by which HIV-1 Gag proteins are targeted and transported to the plasma membrane after ribosomal synthesis is unknown. In this work, we investigated potential interaction of p6 Vpr with model membranes have determined their binding constants. Plasmon waveguide resonance (PWR) experiments showed that strongly interacts (Kd ∼40 nM), may help explaining in part why assembles into coating itself lipids. Moreover, a substantial increased affinity for was observed while...
Nonenveloped virus must penetrate the cellular membrane to access cytoplasm without benefit of fusion. For birnavirus, one peptides present in capsid, pep46 for infectious bursal disease virus, is able induce pores into membranes as an intermediate step birnavirus-penetration pathway. Using osmotic protection experiments, we demonstrate here that and its pore-forming N-terminal moiety (pep22) form different diameters, 5–8 2–4 nm, respectively, showing both moieties participate pore...
In a previous study, we identified 12 conserved domains within pUL89, the small terminase subunit of human cytomegalovirus. A latter study showed that fragment pUL89(580-600) plays an important role in formation complex by interacting with large pUL56. this analysis was performed to solve structure pUL89(568-635) 50% H2O/50% acetonitrile (v/v). We consists four alpha helices, but did not identify any tertiary structure. The 580-600 formed amphipathic helix, which had hydrophobic side highly...
The occurrence of nosocomial infections has been on the rise for past twenty years. Notably, caused by Gram-positive bacteria Staphylococcus aureus represent a major clinical problem, as an increase in antibiotic multi-resistant strains accompanied this rise. There is thus crucial need to find and characterize new antibiotics against bacteria, antibiotic-resistant general. We identified dermaseptin, DMS-DA6, produced skin Mexican frog Pachymedusa dacnicolor, with specific antibacterial...
An efficient strategy for the synthesis of large libraries conformationally defined peptides is reported, using dynamic combinatorial chemistry as a tool to graft amino acid side chains on well-ordered 3D (3-dimension) peptide backbone. Combining rationally designed scaffolds with selection represents an alternative method access structures that are not genetically encodable. This would allow breakthrough discovery protein mimetic unconventional targets which little known.
Cell-penetrating peptides can internalize ubiquitously in many, if not all, cell types. To explore the specific targeting issue of cell-penetrating (CPPs), we studied glycosaminoglycan (GAG)-binding previously identified Otx2 and En2 homeoproteins (HPs), alone or extended with penetratin-like third helix (H3) En2. HPs are indeed known to cells, thanks their GAG-targeting sequence (Joliot et al. 2022; Cardon 2023). We quantified capacity these enter into various lines express different levels...
S10Oral presentations of genetic risk factors, including these 14 novel loci, were common to both European and non-European IBD.However, the amount phenotypic variance explained by loci between populations was clearly heterogeneous, driven a combination differences in effect size allele frequency.Phenotype data available for 3,986 47,799 patients.There male predominance Crohn's disease (69% vs 45%), more ileocolonic (54% vs. 39%) lower colectomy rates UC (4% 18%) than...
An efficient strategy for the synthesis of large libraries conformationnally defined peptides is reported, using dynamic combinatorial chemistry as a tool to graft amino acid side chains on well-ordered 3D peptide backbone. Combining rationnally designed scaffolds with selection represents an alternative method access structures that are not genetically encodable. This would allow breakthrough discovery protein mimetic unconventional targets which little known.
Numerous Collagen Model Peptides (CMPs) have been engineered using proline derivatives substituted at their C(3) and/or C(4) position in order to stabilize or functionalize collagen triple helix mimics. However, no example has reported so far with C(5) substitutions. Here, we introduce a fluorinated CMP incorporating trifluoromethyl groups the of pseudoproline residues. In tripeptide models, our NMR and Molecular Dynamics (MD) studies shown that, when properly arranged, these residues meet...