A5079415533- Probiotics and Fermented Foods
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- Pediatric health and respiratory diseases
- Clostridium difficile and Clostridium perfringens research
- Drug Transport and Resistance Mechanisms
- Biochemical Analysis and Sensing Techniques
- Bacterial biofilms and quorum sensing
- Inflammatory Bowel Disease
- Enzyme Production and Characterization
- Pharmacogenetics and Drug Metabolism
- Diet, Metabolism, and Disease
- Antimicrobial Peptides and Activities
- Pharmacological Effects of Natural Compounds
- Pediatric Hepatobiliary Diseases and Treatments
- Steroid Chemistry and Biochemistry
- Inhalation and Respiratory Drug Delivery
- Digestive system and related health
- Helicobacter pylori-related gastroenterology studies
- Enzyme Catalysis and Immobilization
- Viral gastroenteritis research and epidemiology
- Microscopic Colitis
- Barrier Structure and Function Studies
- Metabolism and Genetic Disorders
- Polysaccharides and Plant Cell Walls
Sorbonne Université
2010-2023
Centre de Recherche Saint-Antoine
2019-2023
Inserm
2010-2022
Sorbonne University Abu Dhabi
2022
Hôpital Saint-Antoine
2017-2020
Assistance Publique – Hôpitaux de Paris
2017-2020
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2020
Laboratoire des Biomolécules
2012-2018
Centre National de la Recherche Scientifique
2015
Centre de Recherche sur l'Inflammation
2015
Gut microbiota metabolises bile acids (BA). As dysbiosis has been reported in inflammatory bowel diseases (IBD), we aim to investigate the impact of IBD-associated on BA metabolism and its influence epithelial cell inflammation response.Faecal serum rates, expressed as a proportion total BA, were assessed by high-performance liquid chromatography tandem mass spectrometry colonic IBD patients (42) healthy subjects (29). The faecal composition was quantitative real-time PCR. Using profiles...
Crohn's disease (CD)-associated dysbiosis is characterised by a loss of Faecalibacterium prausnitzii, whose culture supernatant exerts an anti-inflammatory effect both in vitro and vivo. However, the chemical nature compounds has not yet been determined.Peptidomic analysis using mass spectrometry was applied to F. prausnitzii supernatant. Anti-inflammatory effects identified peptides were tested directly on intestinal epithelial cell lines transfected with plasmid construction coding for...
S. PERRIN, M. WARCHOL, J.P. GRILL AND F. SCHNEIDER. 2001. Aims: To compare the physiological behaviour of Bifidobacterium infantis ATCC 15697 growing on synthetic oligofructose or its components. Methods and Results: The studies were carried out in regulated non-regulated batch cultures semi-synthetic media. Differences between carbohydrate utilization patterns with glucose, fructose, sucrose fructo-oligosaccharides (FOS) determined. Glucose was preferred substrate for growth biomass...
The validity of the in vitro adhesion tests performed with cultured cell lines, was determined this study by comparison results obtained vivo, a previous study. To make experiment were during long period utilization an appropriate medium, to determine capacity adhered strain colonize intestinal tract. It demonstrated that ability adhere and vivo or cells intro similar.
To characterize the beta-fructofuranosidase of Bifidobacterium infantis ATCC 15697 and to compare it with other bacterial beta-fructofuranosidases.The B. was purified 46.8 times over crude extract by anion exchange chromatography, ultrafiltration gel filtration. The sequence 15 amino acid residues NH2 terminal determined. This enzyme a monomeric protein (Mr 70 kDa) invertase activities. isoelectric point pH 4.3, optimum 6.0 pKas (4.5 7.2) two active groups were obtained. activities inhibited...
This study investigated the effect of growth conditions on proteolytic activity a Pseudomonas strain, named sp. LBSA1, isolated from bulk raw milk. It was compared with three chlororaphis and one fluorescens strain culture collections.Bacteriae were grown in minimal salt medium. For all strains, addition 1% (v/v) skim milk to medium sufficient induce protease production 48-h culture. Addition 1 mmol l(-1) calcium chloride permitted detection four strains cultures but not for LBSA1. The five...
Background and aims N-acyl homoserine lactones (AHLs), which are autoinducer quorum-sensing molecules involved in the bacterial communication network, also interact with eukaryotic cells. Searching for these context of inflammatory bowel disease (IBD) is appealing. The our study were to look AHL faecal samples from healthy subjects (HS) IBD patients correlate profiles microbiome investigate effect AHLs interest on epithelial Methods Using mass spectrometry, we characterised HS (n = 26)...
Growth experiments were conducted on Lactobacillus amylovorus DN-112 053 in batch culture, with or without pH regulation. Conjugated bile salt hydrolase (CBSH) activity was examined as a function of culture growth. The CBSH increased during growth but its course depended salts type and conditions. A Lact. mutant isolated from the wild-type strain after mutagenesis N-methyl-N'-nitro-N-nitrosoguanidine. An agar plate assay used to detect mutants activity. In resting cell experiments, showed...
ABSTRACT Faecalibacterium has recently garnered attention for its potential health implications. To better understand role, we developed and assessed real‐time PCR assays detecting quantifying various species in human stool samples from both healthy individuals Crohn's disease patients, either flare or remission. The targeted the Microbial Anti‐inflammatory Molecule (MAM) genes, which encode MAM proteins. These demonstrated 100% species‐specificity using strains six species: prausnitzii ,...
We estimated and compared the action of three selected strains bifidobacteria in a semi-synthetic medium for different degrees polymerization fructo-oligosaccharides contained commercial products derived from chicory inulin: Fibrulose ® F97 (shorter chains), Fibruline Instant (native LC (longer chains). Biomass production lactate acetate were greater when substrate mostly shorter chain fructo-oligosaccharides. Shorter chains first to be consumed, one strain could use longer chains. As degree...
Ursodeoxycholic acid-producing bacteria are of clinical and industrial interest due to the multiple beneficial effects this bile acid on human health. This work reports first isolation 7-epimerizing from feces a healthy volunteer, basis their capacity epimerize primary acid, chenodeoxycholic ursodeoxycholic acid. Five isolates were found be active starting unconjugated its tauro-conjugated homologue, but none these strains could glyco-conjugated form. Biochemical testing 16S ribosomal DNA...
Ursodeoxycholic acid-producing bacteria are of clinical and industrial interest due to the multiple beneficial effects this bile acid on human health. This work reports first isolation 7-epimerizing from feces a healthy volunteer, basis their capacity epimerize primary acid, chenodeoxycholic ursodeoxycholic acid. Five isolates were found be active starting unconjugated its tauro-conjugated homologue, but none these strains could glyco-conjugated form. Biochemical testing 16S ribosomal DNA...
Abstract In the gut ecosystem, microorganisms regulate group behaviour and interplay with host via a molecular system called quorum sensing (QS). The QS molecule 3-oxo-C12:2-HSL, first identified in human microbiota, exerts anti-inflammatory effects could play role inflammatory bowel diseases where dysbiosis has been described. Our aim was to identify which signalling pathways are involved this effect. We observed that 3-oxo-C12:2-HSL decreases expression of pro-inflammatory cytokines such...
The purpose of this work was to study some aspects bile salt toxicity towards bifidobacteria. A strain (Bifidobacterium coryneforme ATCC 25911) selected for its lack conjugated hydrolase activity (CBSH-), and used with three deconjugating strains (CBSH+), their growth viability in the presence two dihydroxylated salts (tauro- glyco-deoxycholic acids). glycoconjugate induced a more significant inhibition four than tauroconjugate. measured at several pH levels. Glycodeoxycholic acid, but not...
The influence of fructooligosaccharides (FOS) and their monomeric components on bile salt resistance Bifidobacterium breve ATCC 15700, Bif. longum 15707 animalis 25527 was examined. neosugars induced fructofuranosidase activities for the degradation these saccharides. For three strains tested growth identical salts had same inhibitory effect whatever carbohydrate used. survival longum, in presence glycodeoxycholic acid depended, however, carbohydrates: toxic effects could be partly...
The intestine is home to the largest microbiota community of human body and strictly regulates its barrier function. Tight junctions (TJ) are major actors intestinal barrier, which impaired in inflammatory bowel disease (IBD), along with an unbalanced composition. With aim identify new involved host-microbiota interplay IBD, we studied N-acyl homoserine lactones (AHL), molecules bacterial quorum sensing, also impact host. We previously identified gut a prominent AHL, 3-oxo-C12:2, lost IBD....
Intestinal dysbiosis is a key feature in the pathogenesis of inflammatory bowel disease (IBD). Acyl-homoserine lactones (AHL) are bacterial quorum-sensing metabolites that may play role changes host cells-gut microbiota interaction observed during IBD. The objective our study was to investigate presence and expression AHL synthases receptor genes human gut ecosystem We used an silico approach, applied Inflammatory Bowel Disease Multi’omics Database comprising metagenomic metatranscriptomic...