Summary Antibiotics are routinely used in modern livestock farming. The manure from medicated animals is for the fertilization of arable crops, which turn leads to accumulation antibiotic resistance genes (ARGs) environment. This a potentially serious public health issue, yet identities bacterial taxa involved ARG persistence as undetermined. Using soil–manure microcosm experiments, we investigated relationship between (i) diverse ARGs and (ii) dynamics community members. We were able...

Edwardsiella tarda is a Gram-negative bacterium that can infect broad range of hosts including humans and fish. Accumulating evidences have indicated E. able to survive replicate in host phagocytes. However, the pathways involved intracellular infection are unclear. In this study, we examined entry endocytic trafficking mouse macrophage cell line RAW264.7. We found entered RAW264.7 multiplied intracellularly robust manner. Cellular invasion was significantly impaired by inhibition clathrin-...

Summary The B acillus cereus group is composed of G ram‐positive spore‐forming bacteria clinical and ecological importance. More than 200 . isolates have been sequenced. However, there are few reports antibiotic resistance genes. This study identified two functional classes macrolide phosphotransferases ( M phs) in the group. C luster A Mphs inactivate 14‐ 15‐membered macrolides while phs 14‐, 15‐ 16‐membered compounds. genomic region surrounding ph gene related to various plasmid sequences,...

Fluoroquinolone resistance in Streptococcus pneumoniae typically arises through specific site mutations, but dynamic variation of mutations the evolution and interaction among these have not been clearly demonstrated. The objectives this study were to investigate dynamics allele frequency populations evolved under fluoroquinolone pressure pervasive interactions present evolutionary trajectories.Thirty-three obtained by serial passages presence antibiotic sequenced using Paired-End Illumina...

In mammals, CD46 is involved in the inactivation of complement by factor I (FI). teleost, study on function very limited. this study, we examined immunological property a molecule (CsCD46) from tongue sole, teleost species with important economic value. We found that recombinant CsCD46 (rCsCD46) interacted FI and inhibited activation an FI-dependent manner. rCsCD46 also bacterial pathogens via different mechanism to responsible for interaction, involving sites. Cellular showed was expressed...

Focal adhesion kinase (FAK) is a promising target for developing more effective anticancer drugs. To better understand the structure-activity relationships and mechanism of actions FAK inhibitors, molecular modeling study using 3D-QSAR, docking, dynamics simulations, binding free energy analysis were conducted. Two types satisfactory 3D-QSAR models generated, comprising CoMFA model (R2cv = 0.528, R2pred 0.7557) CoMSIA 0.757, 0.8362), predicting inhibitory activities novel inhibitors. The...

Edwardsiella piscicida is an important fish pathogen with a broad host that causes substantial economic losses in the aquaculture industry. Ferric uptake regulator (Fur) global transcriptional and contains two typical domains, DNA-binding domain dimerization domain. In previous study, we obtained mutant strain of full-length fur E. piscicida, TX01Δfur, which displayed increased siderophore production stress resistance factors decreased pathogenicity. To further reveal regulatory mechanism...

Triplophysa yarkandensis is a specific cobitidae species that endemic to Xinjiang Tarim River basin, China. The complete mitochondrial genome sequence of T. from Bosten Lake was determined in this study (Accession number MN821008). mitogenome (16,552 bp) consists 22 tRNA genes, 2 ribosomal RNA 13 protein-coding and 1 control region (D-loop region). the provides an important data set for further genetic mechanism classification.

Thymallus brevirostris is a specific grayling species that naturally occurs in Mongolia and Russia Asia. The complete mitochondrial genome of the Mongolian T. was determined (Accession number MH027384) with total length 16,653 nucleotides, which 15,585 nucleotides are coding DNA 1068 non-coding DNA. It has common feature those other respect to structure gene arrangement. contained 13 protein-coding genes, 2 ribosomal RNA 22 transfer tRNA 1 control region (D-loop region). provides an...

The outbreaks of fungal diseases in cultured fish have been severe recent years, which is harmful to the healthy and sustainable development farming. In this study, an investigation was conducted for significant infections 12 species four regions Xinjiang, China, understand distribution local pathogens. Twenty-six strains with pathogenicity were isolated, challenge experiment showed that eight from Changji area had high infection rate eggs. Based on internal transcribed spacer sequence data...

Streptococcus pneumoniae is a primary cause of bacterial infection in humans. Here, we present the complete genome sequence S. strain A026, which multidrug-resistant isolated from cerebrospinal fluid.

Abstract Aims To investigate and identify the antibacterial action mechanism of rose essential oil (REO) against Aeromonas veronii isolated from Northern snakehead for first time by phenotypic metabolic analysis. Methods results The 2-fold broth microdilution spread-plate method identified that minimum inhibitory concentration bactericidal REO A. were 1.25 μl ml−1 impaired growth in a concentration-dependent manner, indicating possessed significant bacteriostatic activity. Electron...

At present, aquatic product pollution has become the main root of frequent food safety problems and causes economic losses. Vibrio is one pathogens causing foodborne diseases. In this study, in order to uncover status pathogenic marine products China, a total 646 were collected analyzed from 10 coastal cities China. Five kinds separated these samples monitored explore relationship between pathogen. Real-time fluorescence quantitative PCR was adopted detect quantitatively products. Aquatic...

is a microalga isolated from the Tarim Basin of Xinjiang, China. The complete mitochondrial genome sequence