Summary Antibiotics are routinely used in modern livestock farming. The manure from medicated animals is for the fertilization of arable crops, which turn leads to accumulation antibiotic resistance genes (ARGs) environment. This a potentially serious public health issue, yet identities bacterial taxa involved ARG persistence as undetermined. Using soil–manure microcosm experiments, we investigated relationship between (i) diverse ARGs and (ii) dynamics community members. We were able...

Abstract The escalating antibiotic resistance crisis poses a major global health threat. Bacteriophage therapy offers promising alternative for combating multidrug‐resistant infections. However, bacterial to phages remains significant hurdle. Innovative strategies are needed overcome this challenge. In study, we developed phage cocktail based on our library, consisting of three that suppressed carbapenem‐resistant hypervirulent Klebsiella pneumoniae (CR‐hvKp). This capitalized dual instances...

Tetracycline antibiotics are widely used in livestock, and tetracycline resistance genes (TRG) frequently reported the manure of farmed animals. However, diversity TRG-carrying transposons has still been rarely investigated. Using a culture-free functional metagenomic procedure, combined with large-insert library construction sequencing, bioinformatic analyses, experiments, we identified 17 distinct TRGs single pig sample, including two new tet genes: tet(59), encoding efflux pump,...

The emergence and spread of antibiotic resistance among Acinetobacter spp. have been investigated extensively. Most studies focused on the multiple genes located plasmids or genomic islands. On other hand, mechanisms controlling intrinsic are still not well understood. In this study, we identified novel subclass aminoglycoside nucleotidyltransferase ANT(3")-II in spp., which comprised numerous variants distributed three main clades. All members can inactivate streptomycin spectinomycin....

Summary The B acillus cereus group is composed of G ram‐positive spore‐forming bacteria clinical and ecological importance. More than 200 . isolates have been sequenced. However, there are few reports antibiotic resistance genes. This study identified two functional classes macrolide phosphotransferases ( M phs) in the group. C luster A Mphs inactivate 14‐ 15‐membered macrolides while phs 14‐, 15‐ 16‐membered compounds. genomic region surrounding ph gene related to various plasmid sequences,...

A novel Gram-stain-negative, strictly aerobic, rod-shaped, gray-pigmented bacterial strain, designated L-25-5 w-1T, was isolated from the water at Baiyang Lake, PR China. Cells of strain w-1T were motile, with a single polar flagellum. able to grow 15-37 °C (optimum 37 °C) and pH 5-8 6) in R2A medium. 16S rRNA gene sequence analysis phylogenetic showed highest relationship Azospirillum doebereinerae GSF71T (97.9 %), thiophilum DSM 21654T %) agricola CC-HIH038T (97.0 other species genus...

Klebsiella pneumoniae carbapenemases (KPCs) are powerful β -lactamases that enable Gram-negative pathogens to destroy clinically important carbapenems in antibiotic therapies. In particular, KPC-2 is difficult detect due a lack of instrument sensitivity regular laboratory screens, which leads misdiagnosis and poor treatment outcomes.

Abstract Background Streptococcus pneumoniae is a gram-positive opportunistic pathogen, and infection risks of S. can be profoundly augmented by its acquired multidrug-resistance (MDR). The rapid development MDR in was attributed to the international dissemination small number multidrug-resistant “clones.” Clonal complex (CC) 271 prevalent CC world most China. However, evolutionary trajectories CC271 China still are largely unknown. Methods We investigated collection 1312 isolates collected...

Abstract Insertion sequences (ISs) promote the transmission of antimicrobial resistance genes (ARGs) across bacterial populations. However, their contributions and dynamics during remain unclear. In this study, we selected IS 26 as a representative transposable element to decipher relationship between ISs ARGs investigate transfer features trends. We retrieved 2656 t ranslocatable ‐b ounded u nits with ARG s (tIS ‐bUs‐ARGs) in complete genomes from NCBI RefSeq database. total, 124 spanning...

Abstract The biogeochemical niche (BN) hypothesis, which states that each species has a specific elemental concentration and stoichiometric proportions, yet to be tested in different organs from plants spanning broad environmental spectrum grasslands. As novel aspect, we the BN hypothesis by analysing leaf root composition (i.e. carbon, nitrogen phosphorus concentrations its ratios), as well morphological traits thickness, area, tissue density, diameter, length density) 139 across 82...

A novel Gram-stain-negative, facultatively anaerobic, rod-shaped, agar-digesting bacterial strain, designated HQM9T, was isolated from the surface of marine red alga Gelidium amansii collected intertidal zone Weihai, China. Cells HQM9T were 3.0-4.0 μm long and 0.2-0.3 wide lacked flagella. The new isolate grew optimally at 28-30 °C, pH 7.0-7.5, in presence 2.5-3.0% NaCl. predominant cellular fatty acids iso-C15 : 0 iso-C17 3-OH. sole menaquinone MK-6. DNA G+C content 33 mol%. major polar...

A filamentous actinomycete strain, designated GW9-2(T), was isolated from a soil sample collected the Fildes Peninsula, King George Island, West Antarctica. The strain identified using polyphasic taxonomic approach. grew slowly on most media tested, producing small amounts of aerial mycelia and no diffusible pigments tested. in presence 0-12 % (w/v) NaCl (optimum, 2-4 %), at pH 9.0-11.0 9.0) 10-37 °C 28 °C). isolate contained meso-diaminopimelic acid, diagnostic sugars MK-9(H4) as...

The genus Exiguobacterium can adapt readily to, and survive in, diverse environments. Our study demonstrated that sp. strain S3-2, isolated from marine sediment, is resistant to five antibiotics. plasmid pMC1 in this carries seven putative resistance genes. We functionally characterized these genes Escherichia coli, encoding dihydrofolate reductase macrolide phosphotransferase were considered novel based on their low similarities known G+C content distribution was highly heterogeneous. Only...

Summary Our ability to predict evolutionary trajectories of pathogens in response antibiotic pressure is one the promising leverage fight against present resistance worldwide crisis. Yet, few studies tackled this question situ at outbreak level, due difficulty link a given pathogenic clone evolution with its precise exposure over time. In study, we monitored real‐time an Aeromonas salmonicida successive and vaccine therapies commercial fish farm. The was responsible for four‐year...

There is an urgent need for efficient tools genetic manipulation to assess plasmid function in clinical drug-resistant bacterial strains. To address this need, we developed all-in-one CRISPR interference (CRISPRi) system that easily inhibited the gene expression of a natural multidrug-resistant sequence type 23 (ST23) Klebsiella pneumoniae isolate. We established integrative CRISPRi plasmid, pdCas9gRNA, harboring dcas9 and single guide RNA (sgRNA) unit under control...

The extensive use of antibiotics in hospitals and the animal breeding industry has promoted antibiotic resistance bacteria, which resulted emergence a large number genes intestinal tract human farmed animals. Genetic exchange between two ecosystems is now well documented for pathogenic but repertoire shared commensal bacterial community by genetic modules they are disseminated still unclear. By analyzing metagenomics data pig samples both collected Shenzhen, China, set 27 highly prevalent...

In this study, we describe strain DCY64T that was isolated from the rhizosphere of three-year-old Korean ginseng root. Cells were Gram-reaction negative, oxidase- and catalase-positive, strictly aerobic, capsulated, non-motile, non-sporulating spherical to short rod-shaped. Multiplicative budding cells produced. Vesicles covered surface cells. Phylogenetic analysis placed within genus Labrys with highest similarity monachus VKM B-1479T (97.6 % 16S rRNA gene sequence similarity), followed by...

The research on molecular biology in ramie [Boehmeria nivea (L.) Gaud.] is not as advanced that major crops due to the lagged development of markers.We compared effectiveness restriction site amplified polymorphism (RSAP), sequence-related (SRAP), and simple sequence repeat (SSR) markers revealing genetic relationship 16 entries germplasm.The SRAP marker detected most polymorphic loci, followed by RSAP marker, SSR had lowest polymorphism.The dendrogram based or data showed high consistency...