A5100414091- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Sexual Differentiation and Disorders
- Physiological and biochemical adaptations
- Animal Genetics and Reproduction
- Identification and Quantification in Food
- Estrogen and related hormone effects
- Phase Change Materials Research
- Genomic variations and chromosomal abnormalities
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Cancer-related gene regulation
- Membrane Separation Technologies
- Hypothalamic control of reproductive hormones
- Radiative Heat Transfer Studies
- Bone Metabolism and Diseases
- Retinal Development and Disorders
- Gender Studies in Language
- MicroRNA in disease regulation
- Animal Behavior and Reproduction
- Wastewater Treatment and Nitrogen Removal
- Advanced Materials and Mechanics
- Cerebrovascular and genetic disorders
Huazhong Agricultural University
2022-2025
Dalian Ocean University
2018-2024
Zhejiang University
2021-2024
Ministry of Education of the People's Republic of China
2020-2023
Children's Hospital of Capital Institute of Pediatrics
2023
He Eye Hospital
2017
University of Southern California
2010
Indiana University – Purdue University Indianapolis
2004
Indiana University School of Medicine
2004
National Institutes of Health
2000
In animals, a trade-off exists between reproduction and growth, which are the most fundamental traits. Males females exhibit profound differences in growth fish species. However, precise molecular mechanism governing this phenomenon is still not clear. Here, we uncovered that chr23-miR-200s dmrt1 knockout specifically caused an impairment increase body female male zebrafish, respectively. Chr23-miR-200s Dmrt1 directly regulate stat5b gene by targeting its 3'UTR promoter. The loss of...
Type XI collagen, a heterotrimer composed of α1(XI), α2(XI) and α3(XI), is primarily synthesized by chondrocytes in cartilage also present some other tissues. collagen plays critical role fibril formation skeletal morphogenesis. We investigated tissue-specific transcriptional enhancer the first intron gene (Col11a2). Transient transfection assays using reporter constructs revealed that 60-base pair (bp) segment within 1 increased promoter activity Col11a2 rat chondrosarcoma cells but not...
To describe a Chinese family with Axenfeld-Rieger syndrome (ARS) and report our novel genetic findings. Nine members of the same underwent complete ophthalmologic examinations analysis. Genomic DNA was isolated from veinal blood amplifed using PCR; products PCR were sequenced compared FOXC1 PITX2 genes, which mutations found. Through examinations, 8 subjects diagnosed as ARS 1 subject normal. A homozygous mutation c.1139_1141dupGCG(p.Gly380_Ala381insGly) heterozygous...
Upon binding to androgen, androgen receptor (AR) can activate expression of target genes through its direct the androgen-responsive elements (AREs), which are located within gene promoters and/or enhancers. A number cellular proteins have been identified as co-regulators regulate this transactivation process. One common structural feature among these is presence LXXLL motif (X, any amino acid), so-called nuclear (NR) box, regulatory AR occurs. We recently shown that Tip110 functions...
Summary Our ability to predict evolutionary trajectories of pathogens in response antibiotic pressure is one the promising leverage fight against present resistance worldwide crisis. Yet, few studies tackled this question situ at outbreak level, due difficulty link a given pathogenic clone evolution with its precise exposure over time. In study, we monitored real‐time an Aeromonas salmonicida successive and vaccine therapies commercial fish farm. The was responsible for four‐year...
Light and temperature are necessary conditions for migratory fish. The assessment of fish physiology behavior is important identifying welfare, but also the optimal setting recirculating aquaculture systems (RASs). This study aimed to explore interactive effect photoperiod on steelhead trout culture. Four treatments were set up with specific settings as follows: a LP-LT group treated 16L:8D 12°C, LP-HT 16°C, SP-LT 12L:12D SP-HT 16°C. Growth performance, behavioral physiological parameters...
Fugu (Takifugu rubripes) is a commercially important fish in aquaculture China, Japan, and Korea. Male fugu are highly prized compared to females because the mature testes regarded as delicacy, thus all-male populations preferred aquaculture. However, molecular mechanisms underlying sex differentiation remain largely unclear. Progestins, which mediated by progesterone receptor, can initiate spermatogenesis or ovarian teleosts. To explore effect of progestin blockage on differentiation,...
Abstract Elucidating the global molecular changes that occur during aromatase inhibitor (AI)– or 17α-methyltestosterone (MT)–induced masculinization and estradiol-17β (E2)–induced feminization is critical to understanding roles endocrine genetic factors play in regulating process of sex differentiation fish. Here, fugu larvae were treated with AI (letrozole), MT, E2 from 25 80 days after hatching (dah), gonadal transcriptomic analysis at dah was performed. The expression dmrt1, gsdf, foxl2,...
To examine the effect and mechanism of thyroid hormone on gonadal sex differentiation, Takifugu rubripes larvae were treated with goitrogen (methimazole, MET, 1000 μg/g), thyroxine (T4, 2nM) from 25 to 80 days after hatching (dah). Gonadal histology ratios fish then determined at dah. MET treatment induced masculinization, but T4 did not induce feminization in T. larvae. Transcriptomic analysis gonads dah was conducted. Among large number differentially expressed genes between groups,...
Naphthalimide photoinduced electron transfer (PET) fluorescent probes are widely used in fluorescence imaging. Thereinto, detection sensitivity is the vital parameter of PET probes. However, modulation yet to be reported for naphthalimide Herein, enhancement through 4-methoxy-substitution proposed this work. Taking Zn2+ an example, 4-methoxy-naphthalimide probe 2-(2-(bis(pyridin-2-ylmethyl)amino)ethyl)-6-methoxy-1H-benzo[de]isoquinoline-1,3(2H)-dione (BPNM) and control...
Abstract A thermoresponsive hydrogel that was composed of 2-hydroxy-3-isopropoxypropyl starch (HIPS) and alginate synthesized via cross-linking with hybrid crosslinkers including ethylene glycol diglycidyl ether (EDGE) calcium chloride (CaCl 2 ). Attenuated total reflection infrared spectroscopy (ATR-IR), thermogravimetric analysis (TGA) derivative thermogravimetry (DTG) were used to confirm occurred. The porous architecture the HIPS/SA composite hydrogels investigated using scanning...
Abstract Background Isodicentric Y chromosome (idic(Y)) is the most commonly reported aberration of human chromosome, which an important cause abnormal sexual development. The breakpoints isodicentric mostly occurred in Yq11.2 and Yp11.3, however, Yq12 are relatively rare. Case presentation We described a 10-year-old boy presenting hypospadias, micropenis short stature, as well unilateral cryptorchidism without normal testicular seminiferous tubules structure by biopsy. Whole exome...
Light has long been known to have a profound influence on the growth and development of fish. The previous studies showed that different spectra had effects juvenile Takifugu rubripes . Among them, green light can promote , but mechanism is unknown. In this study, how LED spectrums affect fish from perspective GH/IGF-I axis-related genes apoptosis were deeply explored. experiment, with an initial body length (9.01 ± 0.70) cm weight (18.05 3.17) g selected as research objects. 525 juveniles...
Abstract Severe aggressive behavior of juvenile pufferfish affects economic efficiency and fish welfare in aquaculture. 5‐HT plays an important role regulating the aquaculture environment. This study examined effects different concentrations (0, 0.25, 0.5, 1 mg/kg) 8‐OH‐DPAT, a selective 1A receptor agonist, on pufferfish. Forty‐five minutes after drug injection, was recorded for 20 min, including latency to first attack frequency behaviors. The results showed no significant differences...
Abstract Ovarian carcinoma is the most lethal arising in female reproductive organ, but exact site of origin unclear. It has long been believed that mesothelial cell layer covers ovarian surface these tumors and its role tumorigenesis still being debated. A central issue this debate potential relationship between coelomic epithelium, from which mesothelium derived, mullerian ducts, organs are derived. We created two transgenes, both driven by Mullerian inhibiting substance type II receptor...