A5067832693- Mesenchymal stem cell research
- Pregnancy and preeclampsia studies
- Congenital Diaphragmatic Hernia Studies
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Birth, Development, and Health
- Phytochemicals and Antioxidant Activities
- Renal and related cancers
- Congenital heart defects research
- Edible Oils Quality and Analysis
- Lymphatic System and Diseases
- Maternal and fetal healthcare
- Prenatal Screening and Diagnostics
- Bone fractures and treatments
- Single-cell and spatial transcriptomics
- Cancer Risks and Factors
- Clinical Nutrition and Gastroenterology
- Building energy efficiency and sustainability
- Osteoarthritis Treatment and Mechanisms
- Reproductive System and Pregnancy
- Energy Efficiency and Management
- Fibroblast Growth Factor Research
- Building Energy and Comfort Optimization
- Tannin, Tannase and Anticancer Activities
University of Auckland
2016-2024
Single-cell technologies (RNA-sequencing, flow cytometry) are critical tools to reveal how cell heterogeneity impacts developmental pathways. The placenta is a fetal exchange organ, containing heterogeneous mix of mesenchymal cells (fibroblasts, myofibroblasts, perivascular, and progenitor cells). Placental stromal (pMSC) also routinely isolated, for therapeutic research purposes. However, our understanding the diverse phenotypes placental lineages, their relationships remain unclear. We...
Olive leaf extract (OLE) has been used for many years its putative health benefits, but, to date, scientific evidence the basis of these effects weak. Although recent literature described a link between ailments such as cardiovascular disease, diabetes and cancer protective effect polyphenols in OLE, mode action is still unclear. Here, we describe double-blinded placebo (PBO)-controlled trial, which gene expression profiles peripheral blood mononuclear cells from healthy male volunteers (n =...
The periosteum plays a crucial role in bone healing and is an important source of skeletal stem progenitor cells. Recent studies mice indicate that diverse populations progenitors contribute to growth, homeostasis healing. Information about the vivo identity diversity cells different compartments adult human skeleton limited. In this study, we compared non-hematopoietic matched tissues from femoral head neck 21 participants using spectral flow cytometry freshly isolated High-dimensional...
Abstract Cell surface marker expression is one of the criteria for defining human mesenchymal stem or stromal cells (MSC) in vitro. However, it unclear if markers including CD73 and CD90 reflects vivo origin cultured cells. We evaluated 15 putative MSC primary from periosteum cartilage to determine whether these either differentiation state self-renewal populations. Cultured had universal consistent various cell > 95% CD73, PDPN both periosteal cultures. Altering culture with...
Placentae from pregnancies with foetal growth restriction (FGR) exhibit poor oxygen and nutrient exchange, in part due to impaired placental vascular development. Placental mesenchymal stromal cells (pMSCs) reside a perivascular niche, where they may influence blood vessel formation/function. However, the role of pMSCs dysfunction FGR is unclear. To elucidate mechanisms by which impact vascularisation we compared transcriptomes human isolated (<5th centile) (n = 7) gestation-matched control...
Abstract Cell surface marker expression is one of the criteria for defining human mesenchymal stem or stromal cells (MSC) in vitro. However, it unclear if markers including CD73 and CD90 reflects vivo origin cultured cells. We evaluated a large panel putative MSC primary from periosteum cartilage to determine whether these either differentiation state self-renewal populations. Cultured had universal consistent various cell >95% CD73, PDPN both periosteal cultures. Altering culture with...
Growth-restricted placentae have a reduced vascular network, impairing exchange of nutrients and oxygen. However, little is known about the differentiation events cell types that underpin normal/abnormal placental formation function. Here, we used 23-colour flow cytometry to characterize vascular/perivascular populations between first trimester term, in foetal growth restriction (FGR). First-trimester endothelial cells had an immature phenotype (CD144+/lowCD36-CD146low), while term expressed...
Abstract Single-cell technologies (RNA-sequencing, flow cytometry) are critical tools to reveal how cell heterogeneity impacts developmental pathways. The placenta is a fetal exchange organ, containing heterogeneous mix of mesenchymal cells (fibroblasts, myofibroblasts, perivascular, and progenitor cells). Placental stromal (pMSC) also routinely isolated, for therapeutic research purposes. However, our understanding the diverse phenotypes placental lineages, their relationships remain...
Abstract Skeletal stem and progenitor cells are critical for bone homeostasis healing, but their identity diversity in humans not well understood. In this study, we compared stromal populations matched tissues from the femoral head neck of 21 human participants using spectral flow cytometry freshly isolated cells. High-level analysis indicated significant differences marker distribution between periosteum, articular cartilage, endosteum marrow populations, identified that were highly...