A5087615168- Mesenchymal stem cell research
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Congenital Diaphragmatic Hernia Studies
- Adipose Tissue and Metabolism
- Pregnancy and preeclampsia studies
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Endometriosis Research and Treatment
- Pluripotent Stem Cells Research
- Adipokines, Inflammation, and Metabolic Diseases
- Proteoglycans and glycosaminoglycans research
- Birth, Development, and Health
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Systemic Lupus Erythematosus Research
- Prenatal Screening and Diagnostics
- CAR-T cell therapy research
- Phytochemical Studies and Bioactivities
- Electrospun Nanofibers in Biomedical Applications
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Renal and related cancers
University of Auckland
2018-2024
University of Otago
2014-2015
Increasing evidence suggests that NK cells act to promote effective T cell-based antitumor responses. Using the B16-OVA melanoma model and an optimized Gram-positive bacteria-dendritic cell (DC) vaccination strategy, we determined in vivo depletion of at time tumor challenge abolished benefit DC immunotherapy. The contribution immunotherapy was dependent on Ag presentation by DC, suggesting as helper prime or reactivate tumor-specific cells. absence resulted greater attenuation immunity than...
Human adipose-derived mesenchymal stromal cells (ASC) are showing clinical promise for the treatment of a range inflammatory and degenerative conditions. These lipoaspirate-derived part abundant accessible source heterogeneous vascular fraction (SVF). They typically isolated expanded from SVF via adherent cell culture at least 2 weeks as such represent relatively undefined population cells. We ex vivo ASC directly lipoaspirate using cocktail antibodies combined with immunomagnetic bead...
Apoptosis leads to the fragmentation and packaging of cellular contents into discrete vesicles, a process known as 'blebbing'. Extracellular vesicles express membrane-bound sialic acids, which enable their capture by CD169 (sialoadhesin; Siglec-1) expressing macrophages in lymph node spleen. Furthermore, mediates vesicle trafficking suppresses immune response exosomes-a type extracellular released from living cells. In this study, we found that CD169(+) were predominant splenic macrophage...
Single-cell technologies (RNA-sequencing, flow cytometry) are critical tools to reveal how cell heterogeneity impacts developmental pathways. The placenta is a fetal exchange organ, containing heterogeneous mix of mesenchymal cells (fibroblasts, myofibroblasts, perivascular, and progenitor cells). Placental stromal (pMSC) also routinely isolated, for therapeutic research purposes. However, our understanding the diverse phenotypes placental lineages, their relationships remain unclear. We...
Mesenchymal stromal cell (MSC)-derived extracellular vesicles (EVs) have shown promise as off-the-shelf therapeutics; however, producing them in sufficient quantities can be challenging. In this study, MSCs were isolated from preimplantation equine embryos and used to produce EVs two commercially available bioreactor designs.Mesenchymal cells (MSC) recently been explored for their potential use therapeutics human veterinary medicine applications, such the treatment of endometrial...
Abstract : Perlecan is a specific proteoglycan that binds to amyloid precursor protein and β‐amyloid peptide, present within deposits, has been implicated in plaque formation. Because formation associated with local inflammation, we hypothesized the mechanisms involved brain inflammatory responses could influence perlecan biosynthesis. To test this hypothesis, first studied regulation mice after inflammation induced by stab wound. mRNA immunoreactivity were both increased 3 days injury....
Several dyes are currently available for use in detecting differentiation of mesenchymal cells into adipocytes. Dyes, such as Oil Red O, cheap, easy to and widely utilized by laboratories analyzing the adipogenic potential cells. However, they not specific changes gene transcription. We have developed a gene-specific assay analyze when cell has switched its fate an lineage. Immuno-labelling against fatty acid binding protein-4 (FABP4), lineage-specific marker differentiation, enabled...
There is clinical interest in using human adipose tissue-derived mesenchymal stromal cells (ASC) to treat a range of inflammatory and regenerative conditions. Aspects ASC biology, including their potential paracrine effect, are likely be modulated, part, by microRNAs, small RNA molecules that embedded as regulators gene-expression most biological pathways. However, the effect standard isolation expansion protocols on microRNA expression not well explored. Here, an untouched enriched...
Several dyes are currently available for use in detecting differentiation of mesenchymal cells into adipocytes. Dyes, such as Oil Red O, cheap, easy to and widely utilized by laboratories analyzing the adipogenic potential cells. However, they not specific changes gene transcription. We have developed a gene-specific assay analyze when cell has switched its fate an lineage. Immuno-labelling against fatty acid binding protein-4 (FABP4), lineage-specific marker differentiation, enabled...
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Abstract Single-cell technologies (RNA-sequencing, flow cytometry) are critical tools to reveal how cell heterogeneity impacts developmental pathways. The placenta is a fetal exchange organ, containing heterogeneous mix of mesenchymal cells (fibroblasts, myofibroblasts, perivascular, and progenitor cells). Placental stromal (pMSC) also routinely isolated, for therapeutic research purposes. However, our understanding the diverse phenotypes placental lineages, their relationships remain...
Abstract Mesenchymal stromal cells (MSC) have recently been explored for their potential use as therapeutics in veterinary medicine applications. Alternatively, MSC-derived extracellular vesicles (EVs) may also provide therapeutic benefits but an off-the-shelf solution, provided they can be produced large enough quantities and without the risk of contamination from bovine EVs contained fetal serum that is a common component cell culture media. Towards this aim, we demonstrated successful...