A5044589609- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Skin and Cellular Biology Research
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Dermatologic Treatments and Research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Wound Healing and Treatments
- Lymphatic System and Diseases
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- melanin and skin pigmentation
- Adipose Tissue and Metabolism
- RNA regulation and disease
- MicroRNA in disease regulation
- Cutaneous Melanoma Detection and Management
- Immune Response and Inflammation
- Silk-based biomaterials and applications
- Conducting polymers and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Peroxisome Proliferator-Activated Receptors
- Skin Protection and Aging
- Barrier Structure and Function Studies
University of Auckland
2013-2025
Maurice Wilkins Centre
2013-2024
Creative Commons
2015
Abstract Gene therapy based on the CRISPR/Cas9 system has emerged as a promising strategy for treating monogenic fragile skin disorder recessive dystrophic epidermolysis bullosa (RDEB). With this approach problematic wounds could be grafted with gene edited, patient‐specific equivalents. Precise editing using homology‐directed repair (HDR) is ultimate goal, however low efficiencies have hindered progress. Reframing strategies highly efficient non‐homologous end joining (NHEJ) aimed at...
Gene editing facilitated by homology-directed repair (HDR) holds great potential for treating monogenetic disorders such as recessive dystrophic epidermolysis bullosa (RDEB). However, low efficiency and variability between loci must be overcome its widespread adoption into personalized therapies. To address these challenges, we developed a highly efficient versatile gene strategy RDEB that incorporates the small molecule inhibitor M3814 to enhance HDR. We focused on three causative COL7A1...
Mesenchymal stem cell (MSC) and progenitor (MPC) populations in human dermis remain poorly characterized, despite their importance to wound repair the pathogenesis of many skin diseases. To identify MSC/MPC we developed an 11-marker flow cytometry technique that enabled sorting mesenchymal for functional assays, using adipose-derived cells (ASCs) from adipose tissue as a positive control. Two dermal had similar phenotypes ASCs: both were CD34(+) CD73(+) CD105(-)/low, lacked expression c-kit...
Abstract The human brain is a highly vascular organ in which the blood-brain barrier (BBB) tightly regulates molecules entering brain. Pericytes are an integral cell type of BBB, regulating integrity, neuroinflammation, angiogenesis and wound repair. Despite their importance, identifying pericytes amongst other perivascular types deciphering specific role neurovasculature remains challenge. Using primary adult cultures fluorescent-activated sorting, we identified two CD73 + CD45 −...
Human adipose-derived mesenchymal stromal cells (ASC) are showing clinical promise for the treatment of a range inflammatory and degenerative conditions. These lipoaspirate-derived part abundant accessible source heterogeneous vascular fraction (SVF). They typically isolated expanded from SVF via adherent cell culture at least 2 weeks as such represent relatively undefined population cells. We ex vivo ASC directly lipoaspirate using cocktail antibodies combined with immunomagnetic bead...
The aim of this study was to further our understanding the transformation process by identifying differentially expressed proteins in melanocytes compared with melanoma cell lines. Tandem mass spectrometry incorporating iTRAQ reagents used as a screen identify and comparatively quantify expression membrane-enriched samples isolated from primary human or three cells Real-time PCR validate significant hits. Immunohistochemistry interest skin melanoma-infiltrated lymph nodes. Publically...
microRNAs (miRNAs) are emerging as key regulators of the immune system, but their role in CD8+ T cell differentiation is not well explored. Some evidence suggests that signals from surface receptors influence expression miRNAs cells, and may have consequent effects on phenotype function. We set out to investigate whether common gamma chain cytokines modulated human miR-146a, which previous studies associated with different stages differentiation. also investigated how changes miR-146a...
The facile preparation of cell-targeted platinum nanoparticles (PtNPs) is described, using designed peptides that as a single molecule control PtNP cluster growth, stabilise clusters in aqueous suspension and enable attachment versatile range cell-targeting ligands.
Abstract Metastasis of human tumors to lymph nodes (LN) is a universally negative prognostic factor. LN stromal cells (SC) play crucial role in enabling T-cell responses, and because tumor metastases modulate their structure function, this interaction may suppress immune responses antigens. The SC subpopulations that respond infiltration malignant into LNs have not been defined. Here, we identify distinctive CD90+ SCs present melanoma-infiltrated compare them with counterparts normal LNs....
One of the key functions human skin is to provide a barrier, protecting body from surrounding environment and maintaining homeostasis internal environment. A mature, stratified epidermis critical achieve barrier function particularly important when producing grafts in vitro for wound treatment. For decades epidermal stratification has been achieved by culturing keratinocytes at an air-liquid interface, triggering proliferating basal differentiate form all layers. We show here that...
Gene editing facilitated by homology-directed repair represents a promising strategy for precisely correcting pathogenic variants underlying monogenic disorders, including the life-threatening skin blistering condition junctional epidermolysis bullosa (JEB). Frequent reports of unintended off-target genotoxicity associated with conventional Cas9 nuclease have increasingly led to adoption dual-Cas9 nickases (dual-Cas9n) owing their improved safety profile. However, rates precise obtained such...
Several dyes are currently available for use in detecting differentiation of mesenchymal cells into adipocytes. Dyes, such as Oil Red O, cheap, easy to and widely utilized by laboratories analyzing the adipogenic potential cells. However, they not specific changes gene transcription. We have developed a gene-specific assay analyze when cell has switched its fate an lineage. Immuno-labelling against fatty acid binding protein-4 (FABP4), lineage-specific marker differentiation, enabled...
There is clinical interest in using human adipose tissue-derived mesenchymal stromal cells (ASC) to treat a range of inflammatory and regenerative conditions. Aspects ASC biology, including their potential paracrine effect, are likely be modulated, part, by microRNAs, small RNA molecules that embedded as regulators gene-expression most biological pathways. However, the effect standard isolation expansion protocols on microRNA expression not well explored. Here, an untouched enriched...
Several dyes are currently available for use in detecting differentiation of mesenchymal cells into adipocytes. Dyes, such as Oil Red O, cheap, easy to and widely utilized by laboratories analyzing the adipogenic potential cells. However, they not specific changes gene transcription. We have developed a gene-specific assay analyze when cell has switched its fate an lineage. Immuno-labelling against fatty acid binding protein-4 (FABP4), lineage-specific marker differentiation, enabled...
Our work on conductive polymer (CP) systems grafted with stimuli-responsive brushes is motivated by the prospect of precisely controlling cellular behaviour tailored smart interfaces. Here, effects cell adhesion applying a potential to poly(3,4-ethylenedioxythiophene) (PEDOT) during both protein coating and culture investigated. The results highlight importance pre-adsorbing fibronectin in this case, especially for reduced which binds strongly. changing surface chemistry PEDOT electrode...