Environmental toxicants such as DDT have been shown to induce the epigenetic transgenerational inheritance of disease (e.g., obesity) through germline. The current study was designed investigate DDT-induced concurrent alterations a number different processes including DNA methylation, non-coding RNA (ncRNA) and histone retention in sperm. Gestating females were exposed transiently during fetal gonadal development, then, directly F1 generation, germline F2 generation F3 sperm investigated....

Epigenetic transgenerational inheritance of disease and phenotypic variation can be induced by several toxicants, such as vinclozolin. This phenomenon involve DNA methylation, non-coding RNA (ncRNA) histone retention, and/or modification in the germline (e.g. sperm). These different epigenetic marks are called epimutations transmit part phenotypes. study was designed to investigate vinclozolin-induced concurrent alterations a number factors, including ncRNA, retention rat sperm. Gestating...

Masculinization depends on the fetal testis. Exposure of human fetus during pregnancy to paracetamol and/or other mild analgesics is associated with an increased risk cryptorchidism. We aimed determine whether disrupted morphology and endocrine function used in vitro system based culture testes exposed or not paracetamol, its metabolite N-(4-hydroxyphenyl)-arachidonoylethanolamide (AM404), aspirin, indomethacin, ketoconazole at 10−4 10−7 M. The study was conducted University Rennes I. Human...

Abstract A variety of environmental toxicants and factors have been shown to induce the epigenetic transgenerational inheritance disease phenotypic variation. Epigenetic alterations in germline (sperm or egg) are required transmit phenotypes. The current study was designed investigate potential role histones sperm help mediate inheritance. agricultural fungicide vinclozolin pesticide DDT (dichlorodiphenyltrichloroethane) were independently used promote disease. Purified cauda epididymal...

Genome-wide DNA methylation analysis is one of the most common epigenetic processes analysed for genome characterization and differential assessment. Previous genome-wide has suggested an important variable in methods involves CpG density. The current study was designed to investigate density a variety different species genomes correlate this various data sets. majority all had >90% low 1–3 CpG/100 bp category, while <10% higher >5 category. Similar observations with human, rat, bird, fish...

Environmentally induced epigenetic transgenerational inheritance of pathology and phenotypic variation has been demonstrated in all organisms investigated from plants to humans. This non-genetic form is mediated through alterations the sperm and/or egg subsequent generations. Although combined regulation differential DNA methylated regions (DMR), non-coding RNA (ncRNA), histone retention (DHR) have shown occur, integration these different processes remains be elucidated. The current study...

Autism spectrum disorder (ASD) has increased over tenfold the past several decades and appears predominantly associated with paternal transmission. Although genetics is anticipated to be a component of ASD etiology, environmental epigenetics now also thought an important factor. Epigenetic alterations, such as DNA methylation, have been correlated ASD. The current study was designed identify methylation signature in sperm potential biomarker offspring autism susceptibility.Sperm samples were...

Abstract Plastic-derived compounds are one of the most frequent daily worldwide exposures. Previously a mixture plastic-derived toxicants composed bisphenol A, bis(2-ethylhexyl) phthalate, and dibutyl phthalate at low-dose exposures gestating female rats was found to promote epigenetic transgenerational inheritance disease offspring (F1 generation), grand-offspring (F2 great-grand-offspring (F3 generation). Epigenetic analysis male sperm result in differential DNA methylation regions (DMRs)...

Abstract Among pregnant women ibuprofen is one of the most frequently used pharmaceutical compounds with up to 28% reporting use. Regardless this, it remains unknown whether could act as an endocrine disruptor reported for fellow analgesics paracetamol and aspirin. To investigate we exposed human fetal testes (7–17 gestational weeks (GW)) using ex vivo culture xenograft systems. Ibuprofen suppressed testosterone Leydig cell hormone INSL3 during 8–9 GW concomitant reduction in expression...

Few studies have been undertaken to assess the possible effects of bisphenol A (BPA) on reproductive hormone balance in animals or humans with often contradictory results. We investigated direct endocrine disruption by BPA fetal testes 2 rat strains (14.5-17.5 days post-coitum) and (8-12 gestational weeks) under different culture conditions. concentrations 10(-8)M 10(-5)M for 72 h reduced testosterone production Sprague-Dawley testes, while only 10-5M suppressed it Wistar strain. The...

A number of environmental factors from nutrition to toxicants have been shown promote the epigenetic transgenerational inheritance disease and phenotypic variation. This requires alterations in germline (sperm or egg) epigenome. Previously, agricultural fungicide vinclozolin was found sperm differential DNA methylation regions (DMRs) termed epimutations that help mediate this inheritance. The current study designed investigate developmental origins DMRs during gametogenesis. Male control...

Because epigenetics is a critical component for gene expression, the hypothesis was tested that DNA methylation alterations are dynamic and continually change throughout gametogenesis to generate mature sperm. Developmental stage-specific during from primordial germ cells (PGCs) sperm investigated. Individual developmental stage were isolated analyzed differential regions (DMRs). The number of DMRs highest in first three comparisons with PGCs, prospermatogonia, spermatogonia. most...

Abstract Environmental toxicants have been shown to promote the epigenetic transgenerational inheritance of disease through exposure specific alterations in germline. The current study examines actions hydrocarbon jet fuel, dioxin, pesticides (permethrin and methoxychlor), plastics, herbicides (glyphosate atrazine) promotion great grand-offspring rats that correlates with associated differential DNA methylation regions (DMRs). observed was similar for all exposures includes pathologies...

Abstract Germline transmission of epigenetic information is a critical component inheritance. Previous studies have suggested that an erasure DNA methylation required to develop stem cells in the morula embryo. An exception involves imprinted genes escape this erasure. Transgenerational differential regions (DMRs) been speculated be imprinted-like and The current study was designed assess if embryos dichlorodiphenyltrichloroethane–induced transgenerational sperm DMR methylation. Observations...

Epigenetic alterations in the germline can be triggered by a number of different environmental factors from diet to toxicants. These environmentally induced changes promote epigenetic transgenerational inheritance disease and phenotypic variation. In previous studies, pesticide DDT was shown sperm differential DNA methylation regions (DMRs), also called epimutations, which part mediate this inheritance. current study, developmental origins DMRs during gametogenesis have been investigated....

The herbicide glyphosate has been shown to promote the epigenetic transgenerational inheritance of pathology and disease in subsequent great-grand offspring (F3 generation). This generational toxicology suggests impacts environmental exposures need assess generations. current study was designed identify biomarkers for glyphosate-induced diseases using an epigenome-wide association (EWAS). Following transient exposure gestating female rats (F0 generation), during developmental period gonadal...

Epigenetic transgenerational inheritance potentially impacts disease etiology, phenotypic variation, and evolution. An increasing number of environmental factors from nutrition to toxicants have been shown promote the epigenetic disease. Previous observations demonstrated that agricultural fungicide vinclozolin pesticide DDT (dichlorodiphenyltrichloroethane) induce sperm epimutations involving DNA methylation, ncRNA, histone modifications or retention. These two were used investigate...

Abstract Abnormal penile foreskin development in hypospadias is the most frequent genital malformation male children, which has increased dramatically recent decades. A number of environmental factors have been shown to be associated with development. The current study investigated role epigenetics etiology and compared mild (distal), moderate (mid shaft), severe (proximal) hypospadias. Penile samples were collected from non-hypospadias individuals identify alterations DNA methylation...

Does ibuprofen use during the first trimester of pregnancy interfere with development human fetal ovary?In fetuses, exposure is deleterious for ovarian germ cells.In utero stages define future reproductive capacity a woman. In rodents, analgesics can impair ovary leading to early onset fertility failure. Ibuprofen, which available over-the-counter, has been reported as frequently consumed medication pregnancy, especially when cells undergo crucial steps proliferation and...

Which transcriptional program triggers sex differentiation in bipotential gonads and downstream cellular events governing fetal testis ovary development humans?The characterization of a dynamically regulated protein-coding non-coding landscape developing human both sexes highlights large number potential key regulators that show an early sexually dimorphic expression pattern.Gonadal is orchestrated by gene XX XY gonads. A comprehensive its counterpart offers promising perspectives for...

Abstract Background Permethrin and N,N-diethyl-meta-toluamide (DEET) are the pesticides insect repellent most commonly used by humans. These have been shown to promote epigenetic transgenerational inheritance of disease in rats. The current study was designed as an epigenome-wide association (EWAS) identify potential sperm DNA methylation epimutation biomarkers for specific disease. Methods Outbred Sprague Dawley gestating female rats (F0) were transiently exposed during fetal gonadal sex...