A5080860665- Reproductive Biology and Fertility
- Effects and risks of endocrine disrupting chemicals
- Sperm and Testicular Function
- Sulfur Compounds in Biology
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- Microplastics and Plastic Pollution
- Infant Nutrition and Health
- Pharmaceutical studies and practices
- Birth, Development, and Health
- Toxic Organic Pollutants Impact
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- Reproductive Physiology in Livestock
- Neuroendocrine regulation and behavior
- Garlic and Onion Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Carcinogens and Genotoxicity Assessment
- Pregnancy and preeclampsia studies
- Selenium in Biological Systems
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Animal Genetics and Reproduction
- Histiocytic Disorders and Treatments
- Reproductive biology and impacts on aquatic species
Charles University
2009-2024
Czech University of Life Sciences Prague
2012-2020
Opća bolnica Karlovac
2020
University of Missouri
2017
Bisphenol A (BPA), a chemical component of plastics, is widely distributed environmental pollutant and contaminant water, air, food that negatively impacts human health. Concerns regarding BPA have led to the use BPA-free alternatives, one which bisphenol S (BPS). However, effects BPS are not well characterized, its specific on reproduction fertility remain unknown. It therefore necessary evaluate any mammalian oocytes. The present study first demonstrate markedly negative pig oocyte...
Bisphenols belong to the endocrine disruptors, affecting reproduction even in extremely low doses. Bisphenol S (BPS) has become widely used as a substitute for earlier-used bisphenol A; however, its harmlessness is questionable. The aim of this study was evaluate effect BPS on folliculogenesis and oocyte quality after vivo exposure doses BPS. Four-week-old ICR females ( n = 16 each experimental group) were exposed vehicle control (VC), BPS1 (0.001 ng BPS.g/bw/day), BPS2 (0.1 ng.g/bw/day),...
Bisphenol S (BPS), the main replacement for bisphenol A (BPA), is thought to be toxic, but limited information available on effects of ovarian follicles. In our study, we demonstrated presence in follicular fluid women at a concentration 22.4 nM. The effect such concentrations oocyte maturation and subsequent embryo development still unknown. Therefore, focused vitro maturation, fertilization, development. As model, used porcine oocytes, which show many physiological similarities human...
The histone code is an established epigenetic regulator of early embryonic development in mammals. lysine residue K9 H3 (H3K9) a prime target SIRT1, member NAD+-dependent deacetylase family enzymes targeting both and non-histone substrates. At present, little known about SIRT1-modulation H3K9 zygotic pronuclei its association with the success preimplantation embryo development. Therefore, we evaluated effect SIRT1 activity on methylation acetylation porcine zygotes significance modifications...
Chromatin remodeling, including histone post-translational modifications, during spermatogenesis can affect sperm quality and fertility, epigenetic marks may therefore be useful for clinical evaluations of sperm. Together with hyperacetylation, the dimethylation H3 on lysine K4 (H3K4me2) is also required protamination. Accordingly, we evaluated utilization this mark identification decrease immature chromatin. In study, 99 semen samples, 22 normozoospermic (N), 63 asthenozoospermic (A), 14...
Hydrogen sulfide (H2S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including reproductive tract. The aim this study was address how exogenous H2S acts on meiotic maturation porcine oocytes, key factors such as MPF and MAPK, cumulus expansion intensity cumulus-oocyte complexes. We observed that donor, Na2S, accelerated oocyte vitro dose-dependent manner, following an increase activity around germinal vesicle breakdown....
SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that involved in oocyte maturation; therefore, the importance of ooplasmic pool for further fate mature oocytes has been strongly suggested. We hypothesised plays role a signalling molecule through selected regulation. observed re-localisation its association with spindle microtubules. In oocytes, distribution shows spindle-like pattern, spindle-specific action decreases α-tubulin acetylation. Based...
Persulfidation contributes to a group of redox post-translational modifications (PTMs), which arise exclusively on the sulfhydryl cysteine as result hydrogen sulfide (H
Porcine oocytes that have matured in vitro conditions undergo the process of aging during prolonged cultivation, which is manifested by spontaneous parthenogenetic activation, lysis or fragmentation aged oocytes. This study focused on role hydrogen sulfide (H2S) porcine oocyte aging. H2S a gaseous signaling molecule and produced endogenously enzymes cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) 3-mercaptopyruvate sulfurtransferase (MPST). We demonstrated H2S-producing are...
Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further culture. Aged remaining at stage undergo spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) lysis. The key factor apoptotic pathway regulation c-Jun-N-terminal kinase (JNK), stress from mitogene-activated protein (MAPK) family. To investigate effect JNK inhibition on porcine ageing, cleavage rate, and...
Abstract Background Bisphenol S (BPS) is increasingly used as a replacement for bisphenol A in the manufacture of products containing polycarbonates and epoxy resins. However, further studies BPS exposure are needed assessment health risks to humans. In this study we assessed potential harmfulness low-dose on reproduction male mice. Methods To simulate human under experimental conditions, 8-week-old outbred ICR mice received 8 weeks drinking water broad range doses [0.001, 1.0, or 100 μg/kg...
Deficient sperm motility is a frequent cause of the age-related male sub-/infertility. Since protein sirtuin 1 (SIRT1) develops anti-aging action and participates in ATP synthesis mitochondria, we investigated its role acquisition hyperactivated during capacitation. For this, dynamics subpopulations were studied, using males Sirt1
Abstract Breastfeeding plays an essential role in the healthy development of a newborn, but human milk is obviously compromised by pollutants from our environment. The main contaminants with endocrine-disrupting compound (EDCs) have raised concern for public and environmental health. Bisphenol A (BPA), which can leach plastics, are among most well-studied. Since EDs known to cross mammary gland barrier BPA may accumulate neonate, “BPA-free” products been introduced market. However, recent...
ABSTRACT Nitric Oxide (NO) has been involved in both intra‐ and extra‐cellular signaling pathways a wide range of organisms, can be detected some reproductive tissues. Based upon previous results reporting that NO‐donor SNAP ( s ‐nitroso‐ n ‐acetyl penicillamine) promoted the release from metaphase II‐anaphase II block amphibian eggs, aim present study was to assess influence on activation molecular mechanisms triggering meiotic resumption Xenopus oocytes, analogous G2/M transition cell...