Nano silver has positive effects on the growth and development of in vitro plants. In this study, shoots banana with 1 cm length were cut off tip which was cultured Murashige Skoog (MS) medium supplemented 30 g.L-1 sucrose, 15% coconut water (v/v), 5 mg. L-1 6-Benzyladenine (BA), 8 agar nano (1, 3, 5, 7 ppm). After twenty days cultured, ppm have grown well 8.4 times multiplication total chlorophyll content (2.05 mg.g-1), three-fold higher than control experiment. All new shoot samples...

Anoectochilus roxburghii Lind. (A. roxburghii) has promising anti-oxidant, hyperglycemic, hepatoprotective, and immunomodulatory activities as well anti-tumor effects. However, the pharmacological actions of in vitro cultured plants remain to be determined. Therefore, objective study was assess cytotoxicity vivo potential toxicity an extract derived from cultivated A. roxburghii, termed iARE. The total flavonoid content predominant compounds were identified quantitatively analyzed. iARE...

Neem cake is a product of the cold pressing from neem kernels to obtain oil. Bio-active substances extracted solutions were evaluated for their potential control root knot nematodes and other pests plants. In this study different concentrations solution was tested against second stage juveniles plant parasitic nematode Meloidogyne spp. four phytopathogenic fungi: Rhizoctonia solani, Sclerotium rolfsii, Collectotrichum Phytopthora capsici. Toxicity extractions represented by EC50 value...

A method for micropropagation was developed Dendrocalamus asper, an economically and environmentally important bamboo. Disinfected seeds were cultured in flasks containing 20 ml of Murashige Skoog's medium (MS) supplemented with BA (1.0-7.0 mg l-1) or kinetin l-1). Multiple shoots (6.53) formed on MS 3.0 l-1 reached 1.49 cm length. Continuous shoot proliferation achieved a The multiplication rate 3.30 fold BA. Propagules excised from multiple transferred to rooting medium. After 4 weeks,...

Recently, there has been a high demand for anti-tumor necrosis factor-α monoclonal antibodies (mAbTNFα) in the treatment of rheumatoid arthritis and other autoimmune diseases. Thus, efficient strategies stable high-producing cell lines need to be established increase antibody production. In this study, we describe an approach establish mAbTNFα clone through optimization expression vectors culture media. The ubiquitous chromatin opening element (UCOE) dihydrofolate reductase (DHFR)-based...

Lan Gấm (Anoectochilus formosanus Hayata) đã được chứng minh là loại dược liệu quý, có tác dụng kích thích hệ miễn dịch, chống tăng đường huyết và bảo vệ gan. Rất nhiều nghiên cứu sử các cao chiết khác nhau để đánh giá hoạt tính sinh học của Gấm. Trong này, chúng tôi in vitro làm lành vết thương chứa polysaccharide (AWE) kháng viêm ethanol (AEE) từ nuôi cấy mô tại Việt Nam. Kết quả cho thấy AWE ở nồng độ 100 μg/mL thể hiện sau 16 giờ xử lý trên hình nguyên bào sợi (fibroblast). khi đó, AEE...

Anoectochilus formosanus Hayata was demonstrated to have a benefit healthy due containing active pharmaceutical ingredients. However, A. is usually processed produce tea bags which would destroy the bioactive compounds because of processing procedure. The aim this study evaluate influence extracted methods including microwave-assisted extraction (MAE), ultrasound-assisted (UAE), and fermentation by Lactobacillus acidophilus ATCC-4356 extract ingredients from formosanus. liquid analyzed total...

This paper presented the results on induction and multiplication of embryogenic calli in liquid medium; somatic embryo, shoot/root morphogenesis embryo through culture tissue medium. The aim this study is to lay out basis for large scale these two kinds tissues with high capable secondary metabolite production due their more or less morphological differentiation status. Leaf disks (about 0.5 × cm) were cultured MS medium 2 mg/l 2,4-D callus induction. Callus was subcultured 1 + NAA 0.2...

An efficient method for plant regeneration in Jatropha curcas L. via primary and secondary somatic embryogenesis culture from ex vitro leaves of 6-month-old plants was presented this study. Leaves were cut into transverse thin cell layers (tTCLs) cultured on MS medium supplemented with kinetin (KIN) at 0.5, 1.0, 1.5, 2.0 mg/l combination indole-3-butyric acid (IBA) 0.1, 1.0 or 2,4-dichlorophenoxyacetic (2,4-D) 1.5 . The highest embryogenic callus formation rate (89.3%) obtained KIN 2,4-D....

In this study, to conserve and exploid properly genetic resources of Anoectochilus Lusidia species, nine DNA barcodess: rbcL, matK, rpoB1, rpoB2, rpoC1, rpoC2, ITS1, ITS2, ITS were used for analysis six species these genera.. The results showed that the amplifications regions different between barcodes ranged from 50%-100%. region ITS1 ITS2 100%, 83.3%, rbcL 50%, matK range 66.7 – rpoB rpoC is 83.3%. Based on sequence, genetics phylogenetic tree constructing relationship discrimination among...

In Vietnam, the somatic embryogenesis method was applied successfully on Jatropha curcas L., in this study, effect of amino acids and spermidine is reported. Some with different concentrations [proline (0; 250; 500; 750; 1000 mg.l-1); glutamine 50; 100; 150; 200 adenine sulphate mg.l-1; 0.01; 0.03; 0.05; 0.08 mg.l-1)] were supplied individually culture medium to examine from callus. The results our study showed that (750 (150 (0.03 mg.l-1) increased embryos formation curcas.

The plant regeneration from the single node or callus culture of black pepper (Piper nigrum L.) was described. Various combinations media, growth regulators and explant sterization were compared. Problems, probably caused by endogenous pathogens associated with tissue exudated, often occurred during establishment culture. method to sterilize plants using antibiotic agent such as cefotaxin For shoot initiation establishment, optimal concentration BA 2mg/l, IBA 0.5 mg/l. high concentrations...

The present paper describes the effect of light intensity, myo-inositol and adenin sulphate on in vitro shoot formation Laba banana (Musa sp.). In shoots were cultured MS medium supplemented with 5 mg l-1 BA (6-benzylaminopurine) different concentrations (0, 100, 300, 500, 700 l-1) or 80, 130, 160 l-1). After 4 weeks, best multiple formed + 100 (6.9 per explant) containing (8.0 explant). Explants suitable concentration maintained under 12-hphotoperiod intensities light: Diffuse light, 18.70...

Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) is a precious with high economical value, which an endemic plant of Vietnam. In this study, experiments on the effects sucrose concentrations and various sizes erlenmeyer flasks bioreactors were carried out to determine appropriate medium for growth cell suspension. The results concentration showed that highest biomass obtained from treatment MS (Murashige Skoog, 1962) supplemented 1.5 mg/L NAA 50 g/L (37.4 mg/mL fresh weight 3.6 dry...


 The embryogenic calli were grown on MS medium containing NaCl with concentrations from 50 to 300 mM. After 2 weeks of culture, salinity tolerance threshold was identified at 150 mM NaCl. Higher stimulated a significant reduction in the survival rate and highest 78.67% subculturing callus embryo culture con- taining NaCl, growth embryogenesis not affected – 100 Especially, reached 83.33%. In contrast, inhibited somatic embryogenesis. 4 weeks, culturing embryos addition 0.07 mg/l...

Physic nut plant (Jatropha curcas L.) is considered as a potential source for non-edible biofuel-producing energy crop throughout the world. This optimized meristem culture technique would be useful developing uniform and virus-free clones of physic plant. shoot apical meristems with 2 leaf primordia were aseptically isolated to sterilize on Murashige Skoog (MS) medium supplemented kinetin (0; 0.1; 0.5; 1.0; 1.5; 2.0 mg.l-1), BA TDZ 0.01; 0.05; 0.10; 0.50; 1.0 mg.l-1) individually. Primary...

Sự phat sinh mo sẹo từ nuoi cấy lớp mỏng tế bao mảnh la ( transverse Thin Cell Layer - tTCL) ở cây hồ tieu đa được ghi nhận. cơ quan chồi co nguồn gốc tTCL tả. Những đam chưa phân hoa trong khối hinh thanh nhu của thịt qua định hướng những đỉnh trưởng va hoan chỉnh. tai triển binh thường tren moi trường MS. Chữ viết tắt: BAP – N6-benzyladenin; IAA 3-indoleacet ic acid; IBA indole -3-butyric MS Murashige and Skoog medium (1962); TCL cell layer; 2,4-D 2,4-Dichlorophenoxy acetic acid.

Currently, fluorescent lamps are often used as lighting systems in the tissue culture. These consumed a lot of power. Recently, use LED lamp for planting was being noticed because LEDs were more conducive to culture and less power consumption. In this study, effects mineral content, sugar concentration monochromatic light on ginger’s growth investigated. The results showed that ginger plant cultured SH medium (Schenk Hildebrandt, 1972) had higher height diameter than other media. experiment...

Anoectochilus formosanus Hayata is a high medicinal as well commercial plant. It necessary to study and apply several organic compounds such yeast extract casein hydrolysate replace inorganic nitrate source in culture medium the process of production vitro A. biomass for supplying safe resource. In this study, shoots were cultured on Albert’s which was decreased nitrate-containing salt, vitamin supplement MS medium, 30 g. L-1 sucrose (yeast hydrolysate) with different concentrations (1.0,...

Lan Kim Tuyến (Anoectochilus roxburghii) là một dược liệu quý với những tác dụng lý đặc biệt đã được chứng minh như chống oxy hóa, kháng viêm, bảo vệ tế bào gan, hạ đường huyết và ung thư. Một quy trình định lượng isorhamnetin trong cao chiết từ cây kim tuyến xây dựng nhằm đánh giá chất nguồn nguyên liệu. Quy sử hệ thống sắc ký lỏng hiệu năng cột pha đảo C18, dung môi động bao gồm acetonitril acid phosphoric 0,2% chế độ đẳng dòng. Nhiệt buồng 30ºC, thể tích tiêm mẫu 10 µl, phát hiện ở bước...

Background and Objective: Dendrobium officinale Kimura et Migo was a precious plant used as folk food traditional medicine.It has proven effective in the treatment of diabetes, anticancer enhancement immune system.To enhance growth accumulation bioactive compound content, effect Light-Emitting Diode (LED), salicylic acid (SA) yeast extract (YE), individual or combination on total phenolic content (TPC) vitro D. plantlets studied.Materials Methods: shoots (0.5 cm) were incubated various...