A5081800677- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Tracheal and airway disorders
- SARS-CoV-2 detection and testing
- Neonatal Respiratory Health Research
- Animal Disease Management and Epidemiology
- Toxoplasma gondii Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer Genomics and Diagnostics
- Heparin-Induced Thrombocytopenia and Thrombosis
- Immune Response and Inflammation
- Multiple Myeloma Research and Treatments
- COVID-19 epidemiological studies
- Virology and Viral Diseases
- Lymphoma Diagnosis and Treatment
- Thermal Regulation in Medicine
- Congenital Diaphragmatic Hernia Studies
- Inhalation and Respiratory Drug Delivery
- Rabies epidemiology and control
- Virus-based gene therapy research
Wageningen University & Research
2015-2024
Fondazione IRCCS Istituto Nazionale dei Tumori
2019
Human respiratory syncytial virus (RSV) is the most important cause of severe lower tract disease (LRTD) in young children worldwide. Extensive neutrophil accumulation lungs and occlusion small airways by DNA-rich mucus plugs are characteristic features RSV-LRTD. Activated neutrophils can release extracellular traps (NETs), networks DNA covered with antimicrobial proteins, as part first-line defence against pathogens. NETs trap eliminate microbes; however, abundant NET formation may also...
Abstract Until universal influenza vaccines become available, pandemic preparedness should include developing classical against potential subtypes. We here show that addition of SWE adjuvant, a squalene-in-water emulsion, to H7N9 split vaccine clearly enhanced functional antibody responses in ferrets. These were cross-reactive strains from different lineages and newly emerged variants. Both formulations protected almost all cases severe pneumonia induced by intratracheal infection ferrets...
In assessing species susceptibility for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and in the search an appropriate animal model, multiple research groups around world inoculated a broad range of using various SARS-CoV-2 strains, doses administration routes. Although silico analyses based on receptor binding diverse vitro cell cultures were valuable, exact prediction these tools proved challenging. Here, we assessed whether precision-cut lung slices (PCLS) could facilitate...
Avian influenza viruses continue to cross the species barrier, and if such become transmissible among humans, it would pose a great threat public health. Since its emergence in China 2013, H7N9 has caused considerable morbidity mortality. In absence of universal vaccine, preparedness includes development subtype-specific vaccines. this study, we developed evaluated ferrets an intranasal live attenuated vaccine (LAIV) against based on A/Leningrad/134/17/57 (H2N2) cold-adapted master donor...
Respiratory syncytial virus (RSV) infection causes a substantial lower-respiratory-tract disease burden in infants, constituting global priority for vaccine development. We evaluated immunogenicity, safety and efficacy of chimpanzee adenovirus (ChAd)-based candidate, ChAd155-RSV, bovine RSV (bRSV) challenge model. This model closely reproduces the pathogenesis/clinical manifestations severe pediatric disease. In seronegative calves, ChAd155-RSV elicits robust neutralizing antibody responses...
Pulmonary function examinations are critical to assess respiratory disease severity in patients. In preclinical rodent models of viral infections, however, is frequently evaluated based on virological, pathological and/or surrogate clinical parameters, which not directly associated with lung function. To bridge the gap between and readouts, we aimed apply unrestrained whole-body plethysmography (WBP) measurements a SARS-CoV-2 Syrian hamster challenge model. While WBP used for research mice...
Abstract Since the original outbreak of SARS-CoV-2 virus, several rapidly spreading variants concern (VOC) have emerged. Here, we show that a single dose Ad26.COV2.S (based on Wuhan-Hu-1 spike variant) protects against Gamma and Delta in naive hamsters, supporting observed maintained vaccine efficacy humans these VOC. Adapted spike-based booster vaccines targeting Omicron now been authorized absence human data. We evaluated immunogenicity Ad26.COV2.S.529 (encoding stabilized BA.1 spike) mice...
The respiratory syncytial virus (RSV) has two main variants with similar impact, a human and bovine variant. (HRSV) is the most frequent cause of acute disease (pneumonia) in children, leading to hospitalization causing premature death. In Europe, lower tract infections caused by HRSV are responsible for 42-45 percent hospital admissions children under two. Likewise, (BRSV) significant viral broncho-pneumonia calves. To date no licensed vaccine been developed, despite high burden disease....
Abstract Respiratory syncytial virus (RSV) infection causes a substantial lower respiratory-tract disease burden in infants, and is global priority for vaccine development. We evaluated the immunogenicity, safety efficacy of chimpanzee adenovirus (ChAd)-based candidate, ChAd155-RSV, bovine RSV (bRSV) challenge model. This model closely reproduces pathogenesis/clinical manifestations severe pediatric disease. In seronegative calves, ChAd155-RSV elicited robust neutralizing antibody responses...
Sensor technologies are increasingly used to monitor laboratory animal behaviour. The aim of this study was investigate the added value using accelerometers and video activity drinking behaviour three rams from 5 days before 22 after inoculation with Toxoplasma gondii. We computed accelerometer data as vectorial dynamic body acceleration (VDBA). In addition, we assessed individual video, frame differencing above drinker identify bouts, Aruco markers for identification. Four inoculation,...
Vaccination with Ad26.RSV.preF, an Adenoviral serotype 26 vector encoding RSV F protein stabilized in its prefusion conformation, has previously shown to be immunogenic and protective seropositive adults infants. Human bovine (bRSV) are genetically highly related share many aspects of pathogenesis, epidemiology clinical manifestations at young age. As such, infection calves bRSV represents a clinically relevant model high translational value, enabling preclinical evaluation Ad26.RSV.preF...
<title>Abstract</title> One of the concerns regarding vaccine safety during COVID-19 pandemic was potential manifestation vaccine-associated enhancement disease (VAED) upon SARS-CoV-2 infection. To investigate suitability Syrian hamster model to test for VAED, we immunized animals with an experimental formaldehyde-inactivated, alum-adjuvanted preparation. In two independent experiments, challenge infection did not result in clinical vaccinated animals. However, at early timepoints (2–5 days)...
Background: Epstein Barr virus (EBV) is associated with the development of a broad range malignancies, including Burkitt's lymphoma, Hodgkin and non‐Hodgkin lymphomas, post‐transplant lymphoproliferative disorder (PTLD), nasopharyngeal carcinoma gastric carcinoma. Although many EBV‐associated lymphomas only express weak immunogenic EBV antigens (e.g. EBNA1 BARF1), type II or III latency often LMP1 LMP2. Different strategies have been developed to manufacture EBV‐LMP1/2‐specific T‐cell...