A5054996924- Sulfur Compounds in Biology
- Genomics, phytochemicals, and oxidative stress
- Air Quality and Health Impacts
- Mercury impact and mitigation studies
- Glutathione Transferases and Polymorphisms
- Heavy Metal Exposure and Toxicity
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Free Radicals and Antioxidants
- Sulfur-Based Synthesis Techniques
- Click Chemistry and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Nerve injury and regeneration
- Heat shock proteins research
- Reproductive Biology and Fertility
- Renal and related cancers
- Sperm and Testicular Function
- Health, Environment, Cognitive Aging
- Selenium in Biological Systems
- Trace Elements in Health
- Spaceflight effects on biology
- Redox biology and oxidative stress
- Toxic Organic Pollutants Impact
National Institute for Minamata Disease
2018-2024
University of Tsukuba
2007-2024
Abstract Methylmercury (MeHg) modifies cellular proteins via their thiol groups in a process referred to as “ S -mercuration”, potentially resulting modulation of the signal transduction pathway. We examined whether low-dose MeHg could affect Akt signaling involved cell survival. Exposure human neuroblastoma SH-SY5Y cells up 2 μM phosphorylated and its downstream molecule CREB, presumably due inactivation PTEN through -mercuration. As result, anti-apoptotic protein Bcl-2 was up-regulated by...
Cadmium (Cd) is an environmental electrophile that modifies protein nucleophiles, thereby modulating cellular signaling and toxicity. While reactive persulfides/polysulfides exhibit relatively high nucleophilic properties, their roles in the altered gene expression toxicity caused by Cd remain unclear. Exposing primary mouse hepatocytes to heat shock 70 (HSP70) metallothionein (MT)-I/II be upregulated cytotoxicity occur. These effects were blocked presence of polysulfide sodium tetrasulfide...
Background: Transcription factor Nrf2 (nuclear factor-erythroid 2-related 2) plays a key role in detoxification of electrophiles via formation glutathione (GSH) adducts and subsequent excretion into extracellular spaces. We found that reactive sulfur species (RSS), such as cysteine persulfides produced by cystathionine γ-lyase (CSE), capture environmental through adducts. However, contributions CSE to the blockage electrophile-mediated toxicity remain be evaluated. Objectives: The aim this...
Abstract Electrophiles can activate redox signal transduction pathways, through actions of effector molecules ( e.g ., kinases and transcription factors) sensor proteins with low p K a thiols that are covalently modified. In this study, we investigated whether 1,4-naphthoquinone (1,4-NQ) could affect the phosphatase tensin homolog (PTEN)–Akt signaling pathway persulfides/polysulfides modulate adaptive response. Simultaneous exposure primary mouse hepatocytes to Na 2 S 4 1,4-NQ markedly...
Reactive sulfur species (RSS) play a role in redox homeostasis; however, adaptive cell responses to excessive intracellular RSS are not well understood. Therefore, this study, we generated transgenic (Tg) mice overexpressing cystathionine gamma-lyase (CSE) produce RSS. Contrary expectations, tissue concentrations of RSS, such as cysteine persulfide (CysSSH), were comparable both wild-type and CSE Tg mice, but the plasma CysSSH significantly higher than mice. This export surplus was also...
The lipid kinase phosphatidylinositol 4-phosphate 5-kinase (PIP5K) produces a versatile signaling phospholipid, 4,5-bisphosphate. Three PIP5K isozymes, PIP5K1A, PIP5K1B, and PIP5K1C, have been identified in mammals so far. Although the functions of these three isozymes extensively studied vitro, vivo physiological roles remain largely unknown. In this study, we examined PIP5K1A PIP5K1B spermatogenesis, using Pip5k1a-knockout (KO), Pip5k1b-KO, Pip5k1a/Pip5k1b double (D)-KO mice. Pip5k1a-KO...
Methylmercury (MeHg), an environmentally toxic substance, causes site-specific neuronal cell death; while MeHg exposure death in cerebrocortical neurons, interestingly, it does not hippocampal which are generally considered to be vulnerable substances. This phenomenon of can reproduced animal experiments; however, the mechanism underlying resistance neurons toxicity has been clarified. In this study, we comparatively analyzed response terms viability and expression characteristics primary...
Brain susceptibility to methylmercury (MeHg) is developmentally and regionally specific in both humans rodents, but the mechanism not well clarified. Reactive sulfur species (RSS) with high nucleophilicity can react MeHg, leading formation of a less toxic metabolite bismethylmercury sulfide, thus exerting cytoprotection. In this study, we assessed variation RSS content rat brain evaluated its relevance sensitivity MeHg. Analyses fetal/juvenile brains showed low levels early developmental...
Environmental electrophiles readily interact with reactive sulfur species (RSS), resulting in decline of cellular RSS levels accompanied by formation their adducts. In the present study, we examined effects combined environmental on consumption persulfide and cytotoxicity HepG2 cells. A convenient assay SSP4, a fluorometric probe for detection per/polysulfides, indicated that each electrophile caused compound-dependent persulfide. Consumption exposure to methylmercury (MeHg) cadmium (Cd) was...
Methylmercury (MeHg) causes selective neuronal damage to cerebrocortical neurons (CCNs) in the central nervous system, but not hippocampal (HiNs), which are highly vulnerable neurodegenerative diseases. In our previous study using cultured rat neurons, we performed a comprehensive gene expression analysis and found that brain-derived neurotrophic factor (BDNF), neurotrophin (NT), was specifically expressed HiNs. Therefore, elucidate causal factors of MeHg toxicity resistance HiNs, conducted...