A5020381751- Nitric Oxide and Endothelin Effects
- Neuroscience and Neuropharmacology Research
- Antibiotics Pharmacokinetics and Efficacy
- Amino Acid Enzymes and Metabolism
- Ion channel regulation and function
- Antibiotic Resistance in Bacteria
- Receptor Mechanisms and Signaling
- Sulfur Compounds in Biology
- Mast cells and histamine
- Protein Kinase Regulation and GTPase Signaling
- Fungal and yeast genetics research
- Neurosurgical Procedures and Complications
- Electron Spin Resonance Studies
- Gas Dynamics and Kinetic Theory
- Cancer therapeutics and mechanisms
- Neuropeptides and Animal Physiology
- Heart Rate Variability and Autonomic Control
- Redox biology and oxidative stress
- Renin-Angiotensin System Studies
- Intracerebral and Subarachnoid Hemorrhage Research
- Plant-based Medicinal Research
- Natural product bioactivities and synthesis
- Diet, Metabolism, and Disease
- Enzyme Structure and Function
- Biochemical effects in animals
University of Occupational and Environmental Health Japan
2025
Yokohama University of Pharmacy
2019-2024
Inserm
2024
Institut Mondor de Recherche Biomédicale
2024
Université Paris-Est Créteil
2024
Showa Pharmaceutical University
2014-2023
Tohoku University
1968-2022
Osaka University of Pharmaceutical Sciences
2022
Juntendo University
2002-2021
University of Tokyo Hospital
2021
Significance Reactive sulfur-containing compounds, such as l -cysteine hydropersulfide (CysSSH), reportedly form in mammals. However, the biological relevance of these reactive sulfur species remains unclear. We determined that CysSSH was synthesized from cystine by cystathionine β-synthase and γ-lyase, which turn may contribute to high levels glutathione (>100 μM) other derivatives peptides/proteins formed cells, tissues, plasma mice humans. Compared with hydrogen sulfide, were superior...
Abstract Cysteine hydropersulfide (CysSSH) occurs in abundant quantities various organisms, yet little is known about its biosynthesis and physiological functions. Extensive persulfide formation apparent cysteine-containing proteins Escherichia coli mammalian cells believed to result from post-translational processes involving hydrogen sulfide-related chemistry. Here we demonstrate effective CysSSH synthesis the substrate l -cysteine, a reaction catalyzed by prokaryotic cysteinyl-tRNA...
Four novel steroidal alkaloids named cortistatins A (1), B (2), C (3), and D (4) consisting of a 9(10−19)-abeo-androstane isoquinoline skeleton have been isolated from the marine sponge Corticium simplex. The absolute stereostructures 1−4 were elucidated by detailed 2D NMR, CD, X-ray crystallographic analyses. Cortistatins A−D inhibited proliferation human umbilical vein endothelial cells (HUVECs) with high selectivity. Among four substances, cortistatin (1) showed strongest...
A unique sequence in the mouse genome which crosshybridized to a cloned human interferon-@, gene was detected by DNA blot analysis.Taking advantage of this, cDNA library prepared from partially purified mRNA for interferon-@ screened using as probe.One positive clones, pM@-3, contained 680-base pair insert, whose base single large open reading frame 182 amino acids.The coding sequences showed homologies 6390 at nucleotide and 48% acid level with respect (Taniguchi, T., Ohno, S.,...
Phosphorylation of neuronal nitric-oxide synthase (nNOS) by Ca2+/calmodulin (CaM)-dependent protein kinases (CaM kinases) including CaM kinase Iα (CaM-K Iα), IIα IIα), and IV IV), was studied. It found that purified recombinant nNOS phosphorylated CaM-K Iα, IIα, at Ser847 in vitro. Replacement with Ala (S847A) prevented phosphorylation kinases. Phosphorylated wild-type (≈0.5 mol phosphate incorporation into nNOS) exhibited a 30% decrease ofV max little change both theK m for l-arginine andK...
We have previously demonstrated that phosphorylation of neuronal nitric-oxide synthase (nNOS) at Ser<sup>847</sup> by Ca<sup>2+</sup>/calmodulin-dependent protein kinases (CaM kinases) attenuates the catalytic activity enzyme <i>in vitro</i> (Hayashi Y., Nishio M., Naito Yokokura H., Nimura Hidaka and Watanabe Y. (1999) <i>J. Biol. Chem.</i> 274, 20597–20602). In present study we determined CaM kinase IIα (CaM-K IIα) can directly phosphorylate nNOS on Ser<sup>847</sup>, leading to a...
ABSTRACT The in vitro and vivo activities of T-3811ME, a novel des-F(6)-quinolone, were evaluated comparison with those some fluoroquinolones, including newly developed one, trovafloxacin. T-3811, free base showed wide range antimicrobial spectra, against Chlamydia trachomatis , Mycoplasma pneumoniae Mycobacterium tuberculosis . In particular, T-3811 exhibited potent activity various gram-positive cocci, MICs at which 90% the isolates are inhibited (MIC 90 s) 0.025 to 6.25 μg/ml. was most...
IFN-gamma genes were introduced through retroviral vectors into the high metastatic, low H-2Kb class I expressor, and poorly immunogenic 3LL-D122 clone. Two types of infectants isolated: secretors (128 to 256 IU/ml) non- or very (< 2 IU/ml). Both manifested expression MHC Ag. showed significant decrease in tumorigenicity metastatic growth, whereas nonsecretors retain more than parental D122 cells. However, both groups, when inoculated an irradiated form, induced similar levels CTL protected...
IFN-gamma is known to induce the expression of Ia antigens on macrophages. We found that murine IFN-alpha and -beta blocked effects in a dose-dependent manner. The antagonistic effect was observed even when macrophages were prestimulated with IFN-gamma. These inhibitory or by their respective antibodies. block exerted IFN-alpha/beta similar whether levels monitored immunofluorescence anti-Ia mAb, stimulation freshly sensitized, alloreactive T lymphoblasts. Adherent macrophage-rich...
The activity of an ethanolic extract Galipea officinalis bark against Mycobacterium tuberculosis was shown to reside mainly in the basic alkaloidal fraction although major part alkaloids present were neutral fraction. Six isolated from including two other not previously reported G. and a new quinoline named allocuspareine, whose structure determined by spectroscopic methods. 1H- 13C-NMR spectral data for three these compounds are first time. Isolation testing fractions individual ten strains...
One of the major steps toward successful islet transplantation for treatment type diabetes is to obtain islets sufficient number and viability. Using a standardized method isolating islets, goal this study was analyze factors influencing outcome isolation. A total 104 cadaveric human pancreata were processed by same team. Data from islet-processing charts reviewed retrospectively. The two endpoints recovery viable after 2 days culture (group V = viable, group NV nonviable) yield. Viable...
A compensation method of target radial motion is formulated and applied to obtain focused images in inverse synthetic aperture radar (ISAR). The basic scheme estimate the centroid compensate such a way that range Doppler are kept constant. Resultant X-band ISAR for ship aircraft indicate estimation accuracy velocity, acceleration, jerk considered be satisfactory.
Squalene epoxidase (SE) (EC 1.14.99.7) catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of rate-limiting enzymes this pathway. Rat SE cDNA was isolated by selecting yeast transformants expressing rat presence terbinafine, an inhibitor specific for fungal SE. The expression terbinafine-resistant clone confirmed its survival either terbinafine or mammalian SE, NB-598, but not both NB-598. polypeptide deduced from nucleotide sequence contains 573 amino...
Survivin is a new member of the inhibitors apoptosis proteins (IAP) family, selectively overexpressed in common human cancers but not normal adult tissues, and associated with aggressiveness disease unfavorable outcomes. Recent study also found that survivin expression cell proliferation. In order to gain insight into role ovarian tumors, we investigated group epithelial examined relationship its proliferation clinical outcome. Immunohistochemical analysis was performed 103 cases tumors....
Dopamine- and cAMP-regulated phosphoprotein of 32 kDa (DARPP-32) plays a central role in medium spiny neurons the neostriatum integration various neurotransmitter signaling pathways. In its Thr-34-phosphorylated form, it acts as potent protein phosphatase-1 inhibitor, and, Thr-75-phosphorylated cAMP-dependent kinase inhibitor. Here, we investigated glutamate-dependent cascades mouse neostriatal slices by analyzing phosphorylation DARPP-32 at Thr-34 Thr-75. Treatment with glutamate (5 mM)...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNMR of silk fibroin. Carbon-13 NMR study the chain dynamics and solution structure Bombyx mori fibroinTetsuo Asakura, Yasuo Watanabe, Akane Uchida, Hidenobu MinagawaCite this: Macromolecules 1984, 17, 5, 1075–1081Publication Date (Print):May 1, 1984Publication History Published online1 May 2002Published inissue 1 1984https://pubs.acs.org/doi/10.1021/ma00135a017https://doi.org/10.1021/ma00135a017research-articleACS PublicationsRequest reuse...
The main purpose of the present study is to envisage molecular mechanism inhibitory action ofdehydrocostuslactone (DCE) andcostunolide (CS), two naturally occurring sesquiterpene lactones, towards activation signal transducer and activator transcription 3 (STAT3). We report that, in human THP-1 cell line, they inhibit IL-6-elicited tyrosine phosphorylation STAT3 its DNA binding activity with EC50 10 µM concomitantdown-regulation ofthe Janus kinases JAK1, JAK2 Tyk2. Furthermore, these...
Abstract Very recent studies indicate that sulfur atoms with oxidation state 0 or −1, called sulfane sulfurs, are the actual mediators of some physiological processes previously considered to be regulated by hydrogen sulfide (H 2 S). 3-Mercaptopyruvate sulfurtransferase (3MST), one three H S-producing enzymes, was also recently shown produce S n ). Here, we report discovery several potent 3MST inhibitors means high-throughput screening (HTS) a large chemical library (174,118 compounds) our...