A5007781413- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
- Circadian rhythm and melatonin
- Insect Resistance and Genetics
- Nutrition, Genetics, and Disease
- CRISPR and Genetic Engineering
- Agricultural pest management studies
- Insect and Pesticide Research
- Mitochondrial Function and Pathology
University of Oregon
2018-2024
Center for Infectious Disease Research
2024
University of Washington
2019-2022
Seattle University
2022
Abstract The goal of the Caenorhabditis Intervention Testing Program is to identify robust and reproducible pro-longevity interventions that are efficacious across genetically diverse cohorts in genus. project design features multiple experimental replicates collected by three different laboratories. Our initial effort employed fully manual survival assays. With an interest increasing throughput, we explored automation with flatbed scanner-based Automated Lifespan Machines (ALMs). We used...
An organism's ability to mount a physiological response external stressors is fundamental its interaction with the environment. Experimental exploration of these interactions benefits greatly from maintain tight control environment, even under conditions in which it would be normal for subject flee stressor. Here we present nematode research platform that pairs automated image acquisition and analysis custom microfluidic device. This enables environmental low-density, single-worm arenas,...
Abstract At the cellular level, many aspects of aging are conserved across species. This has been demonstrated by numerous studies in simple model organisms like Saccharomyces cerevisiae , Caenorhabdits elegans and Drosophila melanogaster . Because most genetic screens examine loss function mutations or decreased expression genes through reverse genetics, essential have often overlooked as potential modulators process. By taking approach increasing level a subset genes, we found that 21%...
The Egg-Counter is a microfluidic research platform for model nematodes that facilitates high precision tracking of reproduction, fundamental process shapes the demography every living organism.
Caenorhabditis elegans (C. elegans) lifespan assays constitute a broadly used approach for investigating the fundamental biology of longevity. Traditional C. require labor-intensive microscopic monitoring individual animals to evaluate life/death over period weeks, making large-scale high throughput studies impractical. The machine developed by Stroustrup et al. (2013) adapted flatbed scanner technologies contribute major technical advance in efficiency survival assays. Introducing platform...
Abstract Small molecule interventions that directly target the molecular mechanisms of aging have potential to revolutionize 21st century health and preventative medicine. Altering trajectory holds incredible economic value in humans, companion pets, other animals. The longevity biotechnology sector is quickly growing meet this opportunity, but a relatively small number targets are poised for clinical development. Ora Biomedical, Inc. pharmaceutical company launched out Kaeberlein Lab at...
The goal of the Caenorhabditis Intervention Testing Program is to identify robust and reproducible pro-longevity interventions that are efficacious across genetically diverse cohorts in genus. project design features multiple experimental replicates collected by three different laboratories. Our initial effort employed fully manual survival assays. With an interest increasing throughput, we explored automation with flatbed scanner-based Automated Lifespan Machines (ALMs). We used ALMs...
Abstract Reproduction is a fundamental process that shapes the demography of every living organism yet often difficult to assess with high precision in animals produce large numbers offspring. Here, we present novel microfluidic research platform for studying Caenorhabditis elegans’ egg-laying. The provides higher throughput than traditional solid-media assays while providing very degree temporal resolution. Additionally, environmental control enabled by animal husbandry allows experimental...
Abstract An organism’s ability to mount a physiological response external stressors is fundamental its interaction with the environment. Experimental exploration of these interactions benefits greatly from maintain tight control environment, even under conditions in which it would be normal for subject flee stressor. ere we present nematode research platform that pairs automated image acquisition and analysis custom microfluidic device. This enables environmental low-density, single-worm...