A5089512446- Fungal and yeast genetics research
- Trace Elements in Health
- Gene Regulatory Network Analysis
- Fermentation and Sensory Analysis
- Fungal Biology and Applications
- Chromium effects and bioremediation
- Environmental Toxicology and Ecotoxicology
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- Analytical chemistry methods development
- Radioactive element chemistry and processing
- Plant Pathogens and Fungal Diseases
- Malaria Research and Control
- 3D Printing in Biomedical Research
- Radioactive contamination and transfer
- Redox biology and oxidative stress
- Metal-Catalyzed Oxygenation Mechanisms
- Diatoms and Algae Research
- Marine Biology and Environmental Chemistry
- Metalloenzymes and iron-sulfur proteins
- Drug Transport and Resistance Mechanisms
- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Characterization
- Aquatic Ecosystems and Phytoplankton Dynamics
- Legionella and Acanthamoeba research
University of Nottingham
2015-2024
The Nature Conservancy
2017
Park University
2014
Georgia State University
1998-2000
Oxford Brookes University
1995-1998
University of Wales
1994-1996
Cardiff University
1995
University of Dundee
1991-1993
Dublin City University
1992-1993
A major cause of the paucity new starting points for drug discovery is lack interaction between academia and industry. Much global resource in biology present universities, whereas focus medicinal chemistry still largely within Open source discovery, with sharing information, clearly a first step towards overcoming this gap. But interface could especially be bridged through scale-up open physical compounds, which would accelerate finding discovery. The Medicines Malaria Venture Box...
Abstract Malaria parasites break down host haemoglobin into peptides and amino acids in the digestive vacuole for export to parasite cytoplasm growth: interrupting this process is central mode of action several antimalarial drugs. Mutations chloroquine (CQ) resistance transporter, pfcrt , located membrane, confer CQ Plasmodium falciparum typically also affect fitness. However, role other loci evolution unclear. Here we use a combination population genomics, genetic crosses gene editing...
The degree of plasma membrane fatty acid unsaturation and the copper sensitivity Saccharomyces cerevisiae are closely correlated. Our objective was to determine whether these effects could be accounted for by differential metal induction lipid peroxidation. S. S150-2B enriched with polyunsaturated acids (PUFAs) linoleate (18:2) linolenate (18:3) growth in 18:2- or 18:3-supplemented medium. Potassium efflux colony count data indicated that both (redox active) cadmium inactive) increased...
One major mechanism of copper toxicity towards microorganisms is disruption plasma membrane integrity. In this study, the influence fatty acid composition on susceptibility Saccharomyces cerevisiae to Cu2+ was investigated. Microbial highly variable, depending both intrinsic and environmental factors. Manipulation achieved in study by growth acid-supplemented medium. Whereas cells grown under standard conditions contained only saturated monounsaturated acids, considerable incorporation...
The GPX1, GPX2, andGPX3 genes of Saccharomyces cerevisiaehave been reported previously to encode glutathione peroxidases (GPxs). We re-examined the sequence alignments these proteins with GPxs from higher eukaryotes. Sequence identities, particularly phospholipid hydroperoxide (PHGPxs), were enhanced markedly by introduction yeast sequences gaps that are characteristic PHGPxs. PHGPx-like activity was detectable in extracts wild-type S. cerevisiae and diminished gpx1Δ, gpx2Δ, andgpx3Δ...
Green fluorescent protein (GFP) has many advantages as a reporter molecule, but its stability makes it unsuitable for monitoring dynamic changes in gene expression, among other applications. Destabilized GFPs have been developed bacterial and mammalian systems to counter this problem. Here, we extend such advances the yeast model. We fused PEST-rich 178 carboxyl-terminal residues of G1 cyclin Cln2 C terminus yEGFP3 (a yeast- FACS-optimized GFP variant), creating yEGFP3-Cln2PEST. tested...
The applicability of the hard-and-soft principle acids and bases in predicting metal adsorption characteristics a biological context was investigated for metabolism-independent uptake ions Sr2+, Mn2+, Zn2+, Cu2+, Cd2+, Tl+ by Saccharomyces cerevisiae. Metal increased with external concentration (5 to 50 microM), although some saturation harder examined, evident at higher concentrations. Cation displacement experiments indicated that, exception Tl+, relative covalent bonding (H+ displacement)...
The yeast <i>Saccharomyces cerevisiae</i>contains two glutaredoxins, encoded by <i>GRX1</i> and<i>GRX2</i>, which are active as glutathione-dependent oxidoreductases. Our studies show that changes in the levels of glutaredoxins affect resistance cells to oxidative stress induced hydroperoxides. Elevating gene dosage of<i>GRX1</i> or <i>GRX2</i> increases hydroperoxides including hydrogen peroxide, <i>tert-</i>butyl hydroperoxide and cumene hydroperoxide. glutaredoxin-mediated is dependent on...
Laboratory and brewing strains of Saccharomyces cerevisiae were compared for metabolism-independent -dependent Sr2+ uptake. Cell surface adsorption to live cells was greater in the than laboratory strain examined. However, uptake levels denatured (dried ground) S. cerevisiae, relative affinities two reversed. Results similar whether organism obtained fresh from brewery waste or after culturing under same conditions as strain. Reciprocal Langmuir plots data biomass not linear, whereas those...
Populations of genetically uniform microorganisms exhibit phenotypic heterogeneity, where individual cells have varying phenotypes. Such phenotypes include fitness-determining traits. Phenotypic heterogeneity has been linked to increased population-level fitness in laboratory studies, but its adaptive significance for wild the natural environment is unknown. Here, we addressed this by testing yeast isolates from diverse environmental sites, each polluted with a different principal...
Abstract To help mitigate large wetland losses in California, The Nature Conservancy launched a dynamic conservation incentive program to create temporary habitats harvested and fallow rice fields for shorebirds migrating along the Pacific Flyway. Farmers were invited participate reverse auction bidding process winning bids selected based on their cost potential provide high quality shorebird habitat. This was done 2014 2015, separate enrollment periods that overlapped with spring fall...
Oxidative damage in microbial cells occurs during exposure to the toxic metal chromium, but it is not certain whether such oxidation accounts for toxicity of Cr. Here, a Saccharomyces cerevisiae sod1Delta mutant (defective Cu,Zn-superoxide dismutase) was found be hypersensitive Cr(VI) under aerobic conditions, this phenotype suppressed anaerobic conditions. Studies with expressing Sod1p variant (Sod1(H46C)) showed that superoxide dismutase activity rather than metal-binding function required...
Increased cellular levels of reactive oxygen species are known to arise during exposure organisms elevated metal concentrations, but the consequences for cells in context toxicity poorly characterized. Using two‐dimensional gel electrophoresis, combined with immunodetection protein carbonyls, we report here that yeast Saccharomyces cerevisiae copper causes a marked increase carbonyl levels, indicative oxidative damage. The response was time dependent, total‐protein oxidation peaking...
Summary Individual cells within isogenic microbial cultures exhibit phenotypic heterogeneity, an issue that is attracting intense interest. Heterogeneity could confer benefits, in generating variant subpopulations may be better equipped to persist during perturbation. We tested this hypothesis by comparing the survival of wild‐type Saccharomyces cerevisiae with mutants which are considered stress‐sensitive but which, we demonstrate, also have increased heterogeneity. The (e.g. vma3 , ctr1...
Summary Growth of Synechocystis PCC 6803 in BG-11 medium supplemented with 1 mM-CsCl resulted intracellular accumulation Cs + to a final level approximately 510 nmol (10 9 cells) -1 after incubation for 10 d. The doubling time was increased by 64% and the cell yield decreased 70% during growth presence as compared control medium. When total monovalent cation concentration doubled adding either K or Na , levels accumulated were 50% 220 270 respectively, 28 d little inhibition being apparent....