A5075685028- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Immune cells in cancer
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Inhalation and Respiratory Drug Delivery
- Pediatric health and respiratory diseases
- Cystic Fibrosis Research Advances
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- NF-κB Signaling Pathways
- Systemic Lupus Erythematosus Research
- Inflammasome and immune disorders
- Glaucoma and retinal disorders
- Virology and Viral Diseases
- Proteoglycans and glycosaminoglycans research
- interferon and immune responses
- Infections and bacterial resistance
- CAR-T cell therapy research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory Support and Mechanisms
- Plant Pathogenic Bacteria Studies
- Kawasaki Disease and Coronary Complications
Lanzhou Veterinary Research Institute
2025
Chinese Academy of Agricultural Sciences
2025
Lanzhou University
2025
Heilongjiang Bayi Agricultural University
2025
Benaroya Research Institute
2009-2024
Seattle University
2023
Virginia Mason Medical Center
2009-2016
University of California, Berkeley
2003-2009
Berkeley College
2006
Autoantibodies to nuclear antigens are hallmarks of systemic lupus erythematosus (SLE) where they contribute pathogenesis. However, there remains a gap in our knowledge regarding how different isotypes autoantibodies this autoimmune disease, including the production critical type I interferon (IFN) cytokines by plasmacytoid dendritic cells (pDCs) response immune complexes (ICs). We focused on IgA, which is second-most prevalent isotype serum and, along with IgG, deposited glomeruli...
Toll-like receptors (TLRs) recognize pathogens and their components, thereby initiating immune responses to infectious organisms. TLR ligation leads the activation of NF-κB MAPKs through well-defined pathways, but it has remained unclear how signaling activates PI3K, which provides an inhibitory pathway limiting responses. Here, we show that adapter B-cell adaptor for PI3K (BCAP) links activation. BCAP-deficient macrophages mice are hyperresponsive agonists have reduced The ability BCAP...
We have previously shown that human tear fluid protects corneal epithelial cells against Pseudomonas aeruginosa in vitro and vivo protection does not depend upon bacteriostatic activity. sought to identify the responsible component(s). The hypothesis tested was collectins (collagenous calcium-dependent lectins) were involved. Reflex collected from healthy subjects examined for collectin content by enzyme-linked immunosorbent assay (ELISA) Western blot with antibody surfactant protein D...
Pseudomonas aeruginosa keratitis is an acute sight-threatening infection. We previously reported that human tear fluid could protect individual corneal epithelial cells in vitro against invasion by and cytotoxicity due to clinical laboratory isolates of P. the protective mechanism was independent bacteriostatic activity. In present study, we examined effects vivo. Tears were collected from healthy volunteers studied vivo mice. The on virulence both invasive cytotoxic examined. Tear found...
CD8+ T cells respond to signals via the cell receptor (TCR), costimulatory molecules, and immunoregulatory cytokines by developing into diverse populations of effector memory cells. The relative strength phosphoinositide 3-kinase (PI3K) signaling early in response can dramatically influence downstream differentiation. We show that initial PI3K during activation results up-regulation scaffold B adaptor for (BCAP), which further potentiates promotes accumulation with a terminally...
The PI3K signaling adaptor BCAP limits inflammasome activation by interacting with the caspase-1 inhibitor Flightless-1.
Abstract Systemic lupus erythematosus severity correlates with elevated serum levels of type I IFNs, cytokines produced in large quantities by plasmacytoid dendritic cells (pDC) response to engagement TLR7 and TLR9 endocytosed nucleic acids. B cell adaptor for PI3K (BCAP) promoted many aspects TLR7-driven lupus-like disease, including Isg15 Ifit1 expression blood an immature pDC phenotype associated higher IFN production. BCAP−/− mice significantly less IFN-α than wild-type after injection...
We reported previously that surfactant protein D (SP-D) was present in human tears and corneal epithelial cells, it contributed to tear fluid protection of those cells against Pseudomonas aeruginosa invasion. This suggested a role ocular innate immunity. Here, we explored the effects bacterial challenge on SP-D expression by cells. Results showed these produced secreted constitutively culture, production (mRNA, protein) secretion were upregulated after exposure heat-killed P. or purified...
Purpose: To use protein arrays to delineate the spectrum of angiogenic bioactive modulators that might be secreted and up-regulated by corneal epithelium in response killed bacterial products. Methods: Immortalized human epithelial cells were grown culture, serum starved, exposed heat-killed Pseudomonas aeruginosa a dose-dependent manner. The resultant culture medium was screened antibody for 43 proteins can modulate angiogenesis immune inflammatory processes. Parallel analysis carried out...
Abstract Autoantibodies to nuclear antigens are hallmarks of the autoimmune disease systemic lupus erythematosus (SLE) where they contribute pathogenesis. However, there remains a gap in our knowledge regarding how different isotypes autoantibodies disease, including production critical type I interferon (IFN) cytokines by plasmacytoid dendritic cells (pDCs) response immune complexes (ICs). We focused on IgA, which is second most prevalent isotype serum, and along with IgG deposited...
Abstract B cell adaptor for phosphoinositide 3-kinase (BCAP) was originally identified as an molecule that binds to the p85 subunit of phosphatidylinositol (PI3K). BCAP-deficient mice have decreased numbers mature cells and attenuated function due defects in receptor signaling. BCAP is also expressed macrophages activates PI3K downstream Toll-like (TLR) ligation. Here we investigated responses stimulation through TLRs. produced higher concentrations inflammatory cytokines response a variety...