A5100687296- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Inflammasome and immune disorders
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Cancer-related molecular mechanisms research
- IL-33, ST2, and ILC Pathways
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Salmonella and Campylobacter epidemiology
- COVID-19 epidemiological studies
- Pediatric health and respiratory diseases
- Escherichia coli research studies
- Dementia and Cognitive Impairment Research
- Neonatal Respiratory Health Research
- Galectins and Cancer Biology
Lanzhou Veterinary Research Institute
2015-2025
Chinese Academy of Agricultural Sciences
2015-2025
Lanzhou University
2022-2025
Hunan Children's Hospital
2021-2025
Hainan Provincial Academy of Agricultural Sciences
2022-2024
Animal Science Research Institute
2022-2024
Xinjiang Agricultural University
2023-2024
Gansu Provincial Center for Disease Control and Prevention
2023-2024
Binzhou People's Hospital
2011-2024
Research Institute of Animal Husbandry
2024
Target identification is highly instructive in defining the biological roles of microRNAs. However, little known about other small noncoding RNAs; for example, tRNA-derived RNA Fragments (tRFs). Some tRFs exhibit a gene-silencing mechanism distinctly different from that typical We recently demonstrated respiratory syncytial virus (RSV)-induced tRF, called tRF5-GluCTC, promotes RSV replication. single most important cause lower tract infection children. By using biochemical screening and...
Acute Respiratory Distress Syndrome (ARDS) is a clinical syndrome characterized by diffuse alveolar damage usually secondary to an intense host inflammatory response of the lung pulmonary or extrapulmonary infectious non-infectious insult often leading development intra-alveolar and interstitial fibrosis. Curcumin, principal curcumoid popular Indian spice turmeric, has been demonstrated as anti-oxidant anti-inflammatory agent in broad spectrum diseases. Using our well-established model...
Intestinal organoids are a newly developed culture system for investigating pathogen-host interactions. organoid models have been widely used since their development, because the results obtained from this type of model better represent physiological conditions than those well-established cell lines. The three-dimensional (3D) porcine intestinal was reported in 2018 and 2019 investigation pathogens. However, were basal-out organoids, which not suitable enteric virus research they invade...
The major enteric RNA viruses in pigs include porcine epidemic diarrhoea virus (PEDV), transmissible gastroenteritis (TGEV), rotavirus A (PRV-A), kobuvirus (PKV), sapovirus (PSaV) and deltacoronavirus (PDCoV). For differential diagnosis, a multiplex RT-PCR method was established on the basis of N genes TGEV, PEDV PDCoV, VP7 gene PRV-A, polyprotein PKV PSaV. This could specifically detect PEDV, PSaV without cross-reaction to any other circulating Chinese pig farms. limit detection this as low...
Swine enteric coronaviruses are a group of most significant pathogens causing diarrhea in piglets with similar clinical symptoms and pathological changes. To develop simple, rapid, accurate, high-throughput detection method for diagnosis differential on swine coronaviruses, specific primers probes were designed based the highly conserved regions transmissible gastroenteritis virus (TGEV) N, porcine epidemic (PEDV) M, deltacoronavirus (PDCoV) alphacoronavirus (PEAV) N genes respectively. A...
Chilling stress increases the amount of polyphenols, especially lignin, which protects tobacco (Nicotiana tabacum L. cv. k326) from chilling stress. To clarify molecular biosynthesis mechanism key representative compounds, specifically RNA sequencing and ultra-high pressure liquid chromatography coupled to quadrupole-time flight mass spectrometry technologies were used construct transcriptomic metabolomic libraries leaves plants subjected normal (25°C) (4°C) temperature treatments....
Disruption of intestinal homeostasis is an important event in the development inflammatory bowel disease (IBD), and genistein (GEN) a candidate medicine to prevent IBD. However, clinical application GEN restricted owing its low oral bioavailability. Herein, reactive oxygen species (ROS)-responsive nanomaterial (defined as GEN-NP2) containing superoxidase dismutase-mimetic temporally conjugated β-cyclodextrin 4-(hydroxymethyl)phenylboronic acid pinacol ester-modified was prepared. GEN-NP2...
ABSTRACT Human noroviruses are responsible for more than 95% of nonbacterial epidemic gastroenteritis worldwide. Both onset and resolution disease symptoms rapid, suggesting that components the innate immune response critical in norovirus control. While study human has been hampered by lack small animal tissue culture systems, our recent discovery a murine (MNV) its vitro propagation have allowed us to begin addressing replication strategies responses infection. We previously demonstrated...
Objective:To explore the feasibility of endoscopic-assisted resection congenital first and second branchial cleft malformations in children via external fistula incision approach. Methods:A retrospective analysis was conducted on 20 with who were admitted to Department Otolaryngology Head Neck Surgery Hu'nan Children's Hospital from January 2020 2024 whose families voluntarily consented endoscopic surgery. Clinical data collected. There 12 males 8 females, aged 10 months years. The surgical...
ABSTRACT Since 2010, highly virulent mutant GII subtype porcine epidemic diarrhea virus (PEDV) strains derived from GI have caused significant economic losses in the pig industry. However, molecular mechanism of PEDV virulence evolution remains unclear. It has been predicted that, compared to S proteins strains, five N-linked glycosylation sites changed strains. To investigate how changes these affect virulence, we constructed recombinant harboring above mutation using rPEDV-S wt as...
The cross-species infection of coronaviruses has resulted in several major epidemics since 2003. Therefore, it is great importance to explore the host ranges and their features among different hosts. In this study, porcine epidemic diarrhea virus (PEDV), with swine as only natural reservoir, was detected rat fecal samples collected from pig farms. Further animal tests showed PEDV can cause systemic infections neonate mice rats. brain, lung intestine spleen were all targets for rodents...
The intestinal epithelium is a physical barrier to enteric viruses and commensal bacteria. It plays an essential role in maintaining the balance between host microenvironment.
Estrogen receptor β (ERβ) and NOD-like family pyrin domain containing 6 (NLRP6) are highly expressed in intestinal tissues. Loss of ERβ NLRP6 exacerbate colitis mouse models; however, the underlying mechanisms incompletely understood. Here, we report that directly activates gene expression via binding to estrogen responsive element Nlrp6 promoter. also physically interacts with nucleotide-binding promotes inflammasome assembly. The ERβ-NLRP6 axis then multiple autophagy-related proteins,...
Small noncoding RNAs (sncRNAs), such as microRNAs (miRNA), virus-derived sncRNAs, and more recently identified tRNA-derived RNA fragments, are critical to posttranscriptional control of genes. Upon viral infection, host cells alter their sncRNA expression a defense mechanism, while viruses can circumvent defenses promote own propagation by affecting cellular or expressing sncRNAs. Therefore, characterizing profiles in response infection is an important tool for understanding host-virus...
Transmissible gastroenteritis virus (TGEV)-induced enteritis is characterized by watery diarrhea, vomiting, and dehydration, has high mortality in newborn piglets, resulting significant economic losses the pig industry worldwide. Conventional cell lines have been used for many years to investigate inflammation induced TGEV, but these may not mimic actual intestinal environment, making it difficult obtain accurate results. In this study, apical-out porcine organoids were employed study...
The ESKAPE pathogens and E. coli are of significant public health concern listed among the top priorities World Health Organization. These equally important for animal health, but their interaction with animal, human, environmental aspects needs to be sufficiently studied. We investigated comprehensive molecular characterization three recovered from milk, farm workers, settings in Hainan province. Minimum inhibitory concentration (MIC), extended-spectrum β-lactamase production, biofilm...
Human noroviruses in the Caliciviridae family are major cause of nonbacterial epidemic gastroenteritis worldwide. Primary human norovirus infection does not elicit lasting protective immunity, a fact that could greatly affect efficacy vaccination strategies. Little is known regarding pathogenesis or immune responses control them because there has previously been no small-animal model cell culture system infection. Using only available infection, we found primary high-dose murine 1 (MNV-1)...