Besides regulating glucose levels, insulin has been reported to participate actively in many other functions such as modulating inflammatory reaction. In this study we investigated how application topically would effort the diabetic wound healing process. We found that excessive expression of degrading enzyme skin led insufficient level during healing, which ultimately reduced recovery rate wounds. confirmed topical could reverse impaired inflammation reaction environment and promote wounds...

Abstract The human hypertrophic scar undergoes hyperplasia and regression during progression. This study aimed to investigate whether fibroblasts in tissue undergo biological changes the formation of scar. Using 32 samples, we measured collagen production by Masson's staining expression levels transforming growth factor ( TGF )‐β1 vascular endothelial VEGF ) immunohistochemistry. In addition, from were isolated cultured, total RNA was extracted for measurement ‐β1 , transcript reverse...

Keratinocyte differentiation dysfunction in diabetic skin is closely related to impaired barrier functions. We investigated the effects of c-Myc and S100A6 on Human immortal keratinocyte line (HaCaT) or potential mechanisms. The expression levels makers such as transglutaminase 1 (TGM1), loricrin (LOR), keratin (K1) were significantly reduced, while was increased HaCaT cells cultured high glucose wound margin keratinocytes from rats human patients. Overexpression caused HaCaT, knocking down...

To investigate the characteristics of diabetic patients with foot ulcers, their health-related quality life (HRQoL), and link between them. The study population included 131 consecutive presenting in a clinic new ulcer December 1, 2011, May 2012. authors collected sociodemographic data, using Wagner Grade, HRQoL (using SF-36 Scale) information; 54.2% were 2 or 3 categories. In all 8 subscales summary scales, had significantly poorer than general China ( P < .01). Their Grade negative...

Most microvessels have been shown to become stenosed or completely occluded during hypertrophic scar progression. Here, we examined the morphology of capillary endothelial cells (ECs) and fibroblasts using immunofluorescence staining for CD31 alpha-smooth muscle actin (α-SMA) electron microscopy. In addition, ECs were isolated from tissues, levels transforming growth factor beta 1 (TGF-β1), platelet-derived (PDGF), endothelin (ET-1), vascular (VEGF) basic fibroblast (bFGF) assayed ELISAs....

Hypertrophic scars (HSs) form due to unchecked proliferation of fibrous tissue after an injury the skin. Recently, lncRNA MIR503HG was shown be involved in HS. However, mechanism by which affects formation and progression HS still needs further study. qRT-PCR applied examine levels miR-143-3p tissues human hypertrophic scar fibroblasts (hHSFs). The relationships MIR503HG, Smad3 were explored with a dual-luciferase reporter assay. Cell proliferation, apoptosis, invasion measured CCK-8 assay,...

Abstract Negative pressure wound therapy (NPWT) has been widely used in various lesions. This study aimed to explore the biological effects of negative on polymorphonuclear neutrophils (PMNs), macrophages, and epidermal keratinocyte cells involved healing. PMNs differentiated from HL‐60, macrophages were derived THP‐1 monocytes, keratinocytes cultured vitro, they treated with 0, −0.03 mp, −0.05 respectively. Cell ultrastructure; viability; apoptosis; protein factors such as tumour necrosis...

Increased accumulation of advanced glycation end products (AGEs) in diabetic skin is closely related to delayed wound healing. Studies have shown that the concentration AGEs elevated tissues and not subcutaneous refractory wounds, which suggests there may be a causal relationship between two. In present study, vitro experiments revealed activated neutrophils, migratory adhesive functions neutrophils decreased once AGE levels reached certain threshold. Different expression differentially...

Objective With the recent exponential increase in express deliveries across China, number of patients with flame burns caused by electric bicycle battery chargers (BEBBC) has markedly increased burn units. In this study, we aimed to characterize BEBBC systematically explore measures prevent their occurrence. Methods We performed a retrospective chart review who visited Burn Department Rui Jin Hospital between January 2015 and December 2021. Results Sixty-three 1412 types other were included...

The legume pod borer, Maruca vitrata (Fabricius, 1787) is a serious pantropical insect pest of grain legumes. emergence and mating rhythms the borer adults were investigated at 29±1°C 75–80% r.h. under 14L:10D. results showed that activity M .vitrata occurred throughout day, 86% females 73% males emerged night. highest frequency 2300–2400 h 0200–0300 for males, respectively. between 2300 2400 3-day-old moths. Single peaks observed 1-, 6-and 7-day-old moths double 2- to 5-day-old percentage...

Cardiovascular disease, one of the most common types disease in clinical practice today, carries a very high risk disability and death. This research aims to examine genome-wide changes injured human dermal microvascular endothelial cells (HDMECs) using Ribonucleic Acid sequencing (RNA-Seq) technique, search for key genes influencing N6-methyladenosine (m6A) methylation, thus gaining new insights into future diagnosis treatment cardiovascular diseases (CVDs) laying foundation follow-up...

Although the etiology underlying scar formation is not well understood, previous studies revealed that endothelial cells play a role in pathogenesis of development. Recently, authors developed reliable technique to obtain from hypertrophic scars involved separation tissue matrix and isolation other cell types. Using phase-contract electron microscopy, were observed have characteristic morphology consistent with origin. The further characterized as by assessment endothelin (ET)-1...

When exposed to thermal factors, collagen in the dermis denatures, which could affect biological behavior of cells. Previous studies have demonstrated that denatured influence activity fibroblasts and induce differentiate into myofibroblasts. However, information on regulation by collagen-modulated autophagy apoptosis during wound healing process is limited. In this article, we researched effect fibroblasts. An vitro model comprising was established identify potential ability modulate...

Objective To study the effect of mechanical tension on collagen arrangement and illustrate relationship between tissue architecture properties. Methods Cell morphologies, orientation fibroblasts cultured in three different types gels with variation were observed by phase contrast photomicrographs, light microscopy transmission electron microscopy. Expression distribution a-smooth muscle actin (α-SMA) investigated immunohistochemistry. Results Phase showed high level distributed...

Severe burns develop a catecholamine surge, inducing severe damage to the organism, raising possibility of multisystem organ failure, and even death. The mechanisms surge have not been fully elucidated, few strategies are generally acceptable reduce postburn. Thus, it is valuable investigate underlying postburn targeted interventions attenuate it. We found that lytic cocktail alleviates injury after burn; however, were still unclear. Moreover, has side effects, such as significant arterial...

Objective To investigate influence of different temperatures on secondary structure type Ⅰ collagen,determine the proper temperature for collagen denaturation,observe changes fibre arrangement and three dimensional gel properties after thermal denaturation,compare morphological variation fibroblasts seeded in mediums with denatured therefore establish a standardized culture model vitro.Methods Changes was measured by circular dichroism spectrameter solution had been treated...

Objective To explore the effect of fecal-adhesion mieromanipulation on biological behavior fibroblast. Methods Micro-pot was made by microcontact printing. The molecules constitutive protein adhered micro-pet self-assemble peptides. Skin fibroblasts were cultured membrane self-made biomechanical cell culture for 2 weeks. Morphology observation and immunohistochemistry analysis performed. Results After weeks, morphology diverse more compliant. Cell found that expression integrinβ11、α5 tensin...

Abstract With the exponential increase in express deliveries over recent years China, battery used for electrical bicycle, is yet hazardous and sometimes leads to fire disaster while charging. The typical burn caused by bicycle charging (BEBBC) has remarkably increased. A retrospective chart review of patient associated with BEBBC collected department Burn Rui Jin Hospital between January 2015 December 2021 were performed. 63 1412 flame patients this study. 56 cases (88.89%) occurred...