A5027807399- Reproductive Biology and Fertility
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
- Tissue Engineering and Regenerative Medicine
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Wound Healing and Treatments
- Renal and related cancers
- Periodontal Regeneration and Treatments
- Genetic and phenotypic traits in livestock
- Cryospheric studies and observations
- Nerve injury and regeneration
- Reproductive Health and Contraception
- Pancreatic function and diabetes
- Veterinary Orthopedics and Neurology
- Prenatal Screening and Diagnostics
- COVID-19 Impact on Reproduction
- 3D Printing in Biomedical Research
- Ovarian function and disorders
- Bone fractures and treatments
- Genetics and Neurodevelopmental Disorders
- Molecular Biology Techniques and Applications
- Seed Germination and Physiology
Indian Veterinary Research Institute
2015-2024
University of Jammu
2023
Geological Survey of India
2022
Indian Council of Agricultural Research
2018-2021
Adesh University
2020
Central Institute for Research on Goats
2016
All India Institute of Medical Sciences
1975-1978
Abstract Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to cell therapy, which needs elucidated. The present study was conducted evaluate the therapeutic potential of caprine, canine, guinea pig bone marrow‐derived MSCs‐CM in excision wound healing model. MSCs were obtained from marrow, expanded ex vivo characterized per ISCT criteria. CM collected assayed by western blot ascertain presence important...
Contents Trace minerals feeding had significant effects on sperm production and fertility with better absorption proper utilization within the body for optimum reproductive function. Several studies have shown that more influenced trace elements in diets of animals are copper (Cu) zinc (Zn). Bucks showing deficiency this mineral might affect quality semen which turn would fertility. This experiment was thus designed to test organic Cu Zn supplementation antioxidants enzyme activities...
The present study was conducted with an objective of isolation, in vitro expansion, growth kinetics, molecular characterization and differentiation fetal adnexa derived caprine mesenchymal stem cells. Mid-gestation gravid uteri (2-3 months) were collected from abattoir to derive cells (MSCs) {amniotic fluid (cAF), amniotic sac (cAS), Wharton's jelly (cWJ) cord blood (cCB)} expanded vitro. These cultured MSCs used at the 3rd passage (P3) localization as well expression specific surface...
This study was conducted to characterize canine bone marrow-derived mesenchymal stem cells (BMSCs); in vivo tracking mice, and therapeutic evaluation clinical paraplegia cases. Canine BMSCs were isolated, cultured, characterized vitro as per International Society for Cellular Therapy criteria, successfully differentiated chondrogenic, osteogenic, adipogenic lineages. To demonstrate the homing property, pGL4.51 vector that contained luciferase reporter gene used transfect BMSCs. Successfully...
The present study was designed to investigate the effect of MSCs-conditioned media (CM) on quality buffalo embryo production in vitro. MSCs were harvested from Wharton's jelly 2-3 month old fetus and CM collected. Immunocytochemistry western blot assay revealed that secrete several important growth factors viz. FGF-2, IGF-1, LIF, TGF-β, VEGF. Slaughterhouse derived culture grade cumulus oocyte complexes (COCs) matured fertilized Presumptive zygotes divided four groups cultured vitro...
This study was conducted to know the impact of cryopreservation on caprine fetal adnexa derived mesenchymal stem cells (MSCs) basic cell characteristics. Gravid uteri (2–3 months) were collected from local abattoir derive (amniotic fluid [cAF], amniotic sac [cAS], Wharton's jelly [cWJ], and cord blood [cCB]) MSCs expanded in vitro. Cells cryopreserved at 3rd passage (P3) using 10% DMSO. Post‐thaw viability cellular properties assessed. determine growth kinetics, tri‐lineage differentiation,...
Adipose-derived mesenchymal stem cells (AdMSC) are multipotent adult isolated and cultured from the stromal vascular fraction derived adipose tissue. The present study was conducted to analyze global trends in AdMSC research using bibliometric visual analysis tools.The literature search done on February 13, 2022, appropriate keywords inclusion-exclusion criteria Scopus database. extracted data were retrospectively analyzed visualized Bibliometrics R packages VOSviewer.Preliminary identified...
Osteoarthritis is a progressive degenerative disease affecting joints. It associated with structural and functional changes that cause lameness pain in dogs. Mesenchymal stem cells (MSCs) are considered an ideal therapeutic candidate for treating inflammatory musculoskeletal conditions due to their paracrine immunomodulatory characteristics. They delivered intravenously or as intra-articular injections canine osteoarthritis. However, ex vivo studies have confirmed the osteoarthritic synovial...
Oxidative stress is one of the leading factors responsible for poor post-thaw semen quality because overproduction reactive oxygen species (ROS) over neutralizing antioxidants present in semen. Mainly two ROS generation sites are spermatozoa, that is, mitochondria and plasma membrane. Therefore, idea targeting these specific minimization production with compounds having known mechanism actions was built up as a core this research.Present study done to investigate effects Mito TEMPO...
Stem cell therapy has been extensively evaluated for its potential in managing neuronal diseases and disorders. The present study was performed to evaluate the therapeutic of allogenic bone marrow-derived mesenchymal stem cells (aBM-MSC) management neural defects associated with vertebral compression fracture (VCF) canine. Six clinical cases presented history secondary non-deviating VCFs were included study. All animals subjected detailed clinical, radiological, haematological investigations...
Oocyte-secreted factors (OSFs) play an important role in the acquisition of oocyte developmental competence through bidirectional cross-talk between and cumulus cells via gap junctions. Thus, present study was designed to investigate effect two OSFs, growth differentiation factor 9 (GDF9) bone morphogenetic protein 15 (BMP15), on buffalo oocytes derived from different follicle sizes. Cumulus-oocyte complexes (COCs) large follicles (LF, >6 mm) or small (SF, 0.05) DOs combination groups....
The objective of this study was to compare the therapeutic potential canine bone marrow derived mesenchymal stem cells (BM MSCs) augmented mesh scaffold for wound healing in guinea pig before and after cryopreservation. Bone aspirate obtained from healthy dogs culture expanded vitro. MSCs were cryopreserved 30 days then used purposes. Both fresh frozen thaw along with purposes pig. No significant (P > 0.05) difference observed population doubling time (PDT) among thawed BM MSCs. expressed...
The consistent, self-renewal capability and wide-ranged differentiation potential during specific physiologic conditions mark stem cells as a novel candidate not only for biomedical research regenerative therapy but also an alternative source in related to life sciences. This vital distinct characteristic of enables them offer unprecedented hope treating many diseases disorders, which are otherwise difficult treat. Several efforts still being undertaken enhance the efficiency MSCs better...