We have synthesized nanowires with an iron core and oxide (magnetite) shell by a facile low-cost fabrication process. The magnetic properties of the can be tuned changing thicknesses to yield remarkable new multi-functionality. A multi-domain state at remanence obtained, which is attractive feature for biomedical applications, where low desirable. also encoded different values. Notably, oxidation process single-crystal halts thickness 10 nm. these acts as passivation layer, retaining even...

Abstract Exploiting and combining different properties of nanomaterials is considered a potential route for next generation cancer therapies. Magnetic nanowires (NWs) have shown good biocompatibility high level cellular internalization. We induced cell death by the chemotherapeutic effect doxorubicin (DOX)-functionalized iron NWs with mechanical disturbance under low frequency alternating magnetic field. (3-aminopropyl)triethoxysilane (APTES) bovine serum albumin (BSA) were separately used...

The Fe(II)-induced ferroptotic cell death pathway is an asset in cancer therapy, yet it calls into question the biocompatibility of magnetic nanoparticles. In latter, Fe(II) sequestered within crystal structure and released only upon nanoparticle degradation, a transition that not well understood. Here, we dissect chemical environment necessary for degradation subsequent release. Importantly, temperature acts as accelerator process can be triggered remotely by laser-mediated photothermal...

Combining different therapies into a single nanomaterial platform is promising approach for achieving more efficient, less invasive, and personalized treatments. Here, we report on the development of such by utilizing nanowires with an iron core oxide shell as drug carriers exploiting their optical magnetic properties. The has large magnetization, which provides foundation low-power manipulation magnetomechanical treatment. enables functionalization doxorubicin through pH-sensitive linker,...

ABSTRACT This study presents a novel method and device for the hydrodynamic production of extracellular vesicles (EVs) derived from biomimetic multicellular 3D spheroids, enabling high-throughput particle release that is 10 to 20 times higher than in non-stimulated conditions. The facilitates formation spheroids human mesenchymal stem cells (hMSCs), offering an all-in-one approach both spheroid generation EV release. Production are reduced just few hours, with yield further increased by...

Abstract Highly efficient magnetic release from nanocomposite microparticles is shown, which are made of Poly (N-isopropylacrylamide) hydrogel with embedded iron nanowires. A simple microfluidic technique was adopted to fabricate the a high control nanowire concentration and in relatively short time compared chemical synthesis methods. The thermoresponsive were used for remotely triggered Rhodamine (B). With field only 1 mT 20 kHz drug 6.5% 70% achieved continuous pulsatile modes,...

Biocompatible functional materials play a significant role in drug delivery, tissue engineering and single cell analysis.We utilized 3D printing to produce high aspect ratio polymer resist microneedles on silicon substrate functionalized them by iron coating.Two-photon polymerization lithography has been used for cylindrical, pyramidal, conical needles from drop cast IP-DIP resist.Experiments with cells were conducted cylindrical 630  15 nm diameter an of 1:10 pitch 12 m.The have arranged...

The increasing interest in the use of magnetic nanostructures for biomedical applications necessitates rigorous studies to be carried out order determine their potential toxicity. This work attempts elucidate cytotoxic effects nickel nanowires (NWs) human fibroblasts WI-38 by a colorimetric assay (MTT) under two different parameters: NW concentration and exposure time. was complemented with TEM confocal images assess NWs internalization identify any changes cell morphology. Ni were...

Abstract Three-dimensional tissue culture, and particularly spheroid models, have recently been recognized as highly relevant in drug screening, toxicity assessment engineering due to their superior complexity heterogeneity akin the vivo microenvironment. However, limitations size control, shape reproducibility long maturation times hinder full applicability. Here, we report a formation technique based on magnetic aggregation of cells with internalized nanoparticles. The method yields...

The assessment of cytotoxicity nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis, electron microscopy. After exposure to the NWs, was evaluated in terms cell viability, membrane damage, induced apoptosis/necrosis on model human line...

Abstract Pluripotent stem cells are central tools to many regenerative medicine strategies due their ability differentiate toward the three embryonic germ layers. One challenge remains in providing control over differentiation into specific lineages, such as cardiac commitment. Here, possibility of directing cardiomyogenesis using a microfabricated magnetic pattern is demonstrated. The labeled with nanoparticles, aggregated embryoid bodies (EBs) onto pattern, and stimulated local force...

A growing tumor is submitted to ever-evolving mechanical stress. Endoscopic procedures add additional constraints. However, the impact of forces on cancer progression still debated. Herein, a set magnetic methods proposed form spheroids and subject them remote deformation, mimicking stent-imposed compression. Upon application permanent magnet, (formed from colon cells or glioblastoma cells) are compressed by 50% their initial diameters. Such significant deformation triggers an increase in...

Iron oxide nanostructures have been widely developed for biomedical applications because of their magnetic properties and biocompatibility. In clinical applications, stabilization these against aggregation nonspecific interactions is typically achieved using weakly anchored polysaccharides, with better-defined more strongly synthetic polymers not commercially adopted complexity synthesis use. Here, we show the first time biocompatibilization iron nanoparticles by a homopolymer strong surface...

Nanostructures fabricated by different methods have become increasingly important for various applications in biology and medicine, such as agents medical imaging or cancer therapy. In order to understand their interaction with living cells internalization kinetics, several attempts been made tagging them. Although developed measure the number of nanostructures internalized cells, there are only few approaches aimed that internalize nanostructures, they usually limited fixed-cell studies....

The interactions between nanoparticles and the biological environment have long been studied, with toxicological assays being most common experimental route. In parallel, recent growing evidence has brought into light important role that cell mechanics play in numerous processes. However, despite prevalence of nanotechnology applications biology, particular increased use magnetic for therapy imaging, impact on cells' mechanical properties remains poorly understood.Here, we used a parallel...

Nanotopographical cues play a critical role as drivers of mesenchymal stem cell differentiation. Nanowire scaffolds, in this regard, provide unique and adaptable nanostructured surfaces with focal points for adhesion elastic properties determined by nanowire stiffness.We show that scaffold nanowires, which are remotely actuated magnetic field, mechanically stimulates cells. Osteopontin, marker osteogenesis onset, was expressed after cells were cultured 1 week on top the scaffold. Applying...

The combined passive and active targeting of tumoral tissue remains an relevant cancer research field. Here, we exploit the properties two highly magnetic nanomaterials, magnetosomes ultramagnetic liposomes, in order to magnetically target prostate adenocarcinoma tumors, implanted orthotopically or subcutaneously, take into account role tumor vascularization efficiency. Analysis organ biodistribution vivo revealed that, for all conditions, both nanomaterials accumulate mostly liver spleen,...

Engineered 3D brain-like models have advanced the understanding of neurological mechanisms and disease, yet their mechanical signature, while fundamental for brain function, remains understudied. The surface tension instance controls development is a marker cell-cell interactions. Here, magnetic tissue spheroids composed intermixed primary glial neuronal cells at different ratios are engineered. Remarkably, two cell types self-assemble into functional tissue, with sorting toward periphery...

Stem cells have been shown to respond extracellular mechanical stimuli by regulating their fate through the activation of specific signaling pathways. In this work, an array iron nanowires (NWs) aligned perpendicularly surface was fabricated pulsed electrodepositon in porous alumina templates followed a partial removal reveal 2-3 μm NWs. This resulted substrates with densely arranged NWs 33 nm diameter separated 100 nm. The were characterized scanning electron microscopy (SEM) energy...

Methods that provide controlled influx of molecules into cells are critical importance for uncovering cellular mechanisms, drug development and synthetic biology. However, reliable intracellular delivery without adversely affecting the is a major challenge. We developed platform on-demand applications, with which cell membrane penetration achieved by inductive heating micro needles. The needles around 1 μm in diameter 5 length made gold using silicon-based fabrication process provides...

A thiol-epoxy click chemistry reaction is used to introduce thiol groups on magnetic nanogels for the attachment of Au nanorods.

Tissue engineering aims to repair or replace deficient tissue by delivering constructs that mimic the native in vivo structure. One challenge cardiac approaches is achieve intrinsic organization, particularly alignment of cardiomyocytes. Here, we propose a strategy for 3D manipulation and cardiomyocytes combining magnetism hydrogel. The advantage using magnetic forces they act remotely on cells when these are endowed with magnetization via internalization nanoparticles. actuation then allows...