A5001204528- Marine Biology and Ecology Research
- Identification and Quantification in Food
- Environmental DNA in Biodiversity Studies
- Marine Ecology and Invasive Species
- Isotope Analysis in Ecology
- Coral and Marine Ecosystems Studies
- Microbial Community Ecology and Physiology
- Marine and coastal plant biology
- Genomics and Phylogenetic Studies
- Insect Pheromone Research and Control
- Machine Learning in Bioinformatics
- Plant and Fungal Species Descriptions
- Marine and fisheries research
- Aquatic Invertebrate Ecology and Behavior
- Marine and environmental studies
- Crustacean biology and ecology
- Parasite Biology and Host Interactions
- Genetic diversity and population structure
Advanced Neural Dynamics (United States)
2024
University of Minho
2013-2023
University of Aveiro
2021
Universidad de La Laguna
2021
Building reference libraries of DNA barcodes is relatively straightforward when specifically designed primers are available to amplify the COI-5P region from a narrow taxonomic group (e.g. single class or order). barcoding marine communities have been comparatively harder accomplish due broad diversity and lack consistently efficient primers. Although some so-called “universal” successful, they still fail many animal groups, while displaying random success even among species within each...
Molecular data have been suggesting the existence of a complex cryptic species within taxon Perinereis cultrifera , which has not fully explored yet. In this study, we performed morphological and molecular analysis (mtCOI-5P 16S rRNA 28SD2 ) specimens from intertidal marine brackish European localities, mostly focusing on Mediterranean Sea Canary Islands. Two major phylogenetic clades with at least 18 divergent (COI 19.8; 6.4-28.5%) completely sorted lineages were uncovered based original...
Abstract Annelid polychaetes have been seldom the focus of dedicated DNA barcoding studies, despite their ecological relevance and often dominance, particularly in soft‐bottom estuarine coastal marine ecosystems. Here, we report first assessment performance barcodes discrimination shallow water polychaete species from southern European Atlantic coast, focusing on specimens collected estuaries ecosystems Portugal. We analysed cytochrome oxidase I ( COI ‐5P) 164 specimens, which were assigned...
DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy species-level assignment, consequent taxonomic coverage, relies on comprehensive barcode reference libraries, which, due to incompleteness, are currently recognized limitation for applications. In this study, we assembled library barcodes Atlantic Iberia marine species, assessed gaps in species coverage examined data ambiguities. Initially, an Iberian checklist the three dominant...
Phyllodocida is a clade of errantiate annelids characterized by having ventral sensory palps, anterior enlarged cirri, axial muscular proboscis, compound chaetae (if present) with single ligament, and lacking dorsolateral folds. Members most families date back to the Carboniferous, although earliest fossil was dated from Devonian. holds 27 well-established morphologically homogenous clades ranked as families, gathering more than 4600 currently accepted nominal species. Among them, Syllidae...
The accuracy of specimen identification through DNA barcoding and metabarcoding relies on reference libraries containing records with reliable taxonomy sequence quality. considerable growth in barcode data requires stringent curation, especially taxonomically difficult groups such as marine invertebrates. A major effort curating the Barcode Life Data Systems (BOLD) was undertaken during 8 th International Conference (Trondheim, Norway, 2019). Major taxonomic (crustaceans, echinoderms,...
Introduction Maritime traffic and coastal urbanisation significantly contribute to the introduction proliferation of non-indigenous species (NIS). However, lack information might prevent effective monitoring in data-limited regions, particularly areas experiencing demographic growth, where biofouling communities could offer crucial insights into dynamics NIS invasions. This study represents a baseline characterization northern Saudi Arabian Red Sea (NEOM region) prior extensive urban,...
In this study we compared DNA barcode-suggested species boundaries with morphology-based identifications in the amphipod fauna of southern European Atlantic coast. sequences cytochrome c oxidase subunit I barcode region (COI-5P) were generated for 43 morphospecies (178 specimens) collected along Portuguese coast which, together publicly available COI-5P sequences, produced a final dataset comprising 68 and 295 sequences. Seventy-five BINs (Barcode Index Numbers) assigned to these...
Past molecular studies using mtDNA sequences and alloenzymes signalled the existence of at least two cryptic species within Hediste diversicolor morphotype, in European coasts. However, to this day, no new descriptions have been made. In study, we identified five completely sorted lineages a multi-locus approach, including mitochondrial DNA cytochrome oxidase I gene (COI-5P) nuclear markers ITS2 rRNA 28S rRNA. The data were complemented with morphometric measurements examined through...
Annelid biodiversity studies in the Red Sea are limited and integrative taxonomy is needed to accurately improve reference libraries region. As part of bioblitz effort Saudi Arabia assess invertebrate northern Gulf Aqaba, Perinereis specimens from intertidal marine lagoon-like rocky environments were selected for an independent assessment, given known taxonomic ambiguities this genus. This study used approach, combining molecular with morphological geographic data. Our results demonstrate...
Abstract We report on two new lineages of the Eumida sanguinea complex from Great Britain and describe one them as a species using multilocus approach, including mitochondrial DNA COI‐5P nuclear markers ITS (ITS1, 5.8S rRNA ITS2) 28S rRNA. The molecular analysis placed mackiei sp. nov. in monophyletic clade with 19.1% (COI), 10.1% (ITS) 1.7% (28S) mean distance to its nearest neighbour. Molecular diagnoses were also applied nine within E. complex. This was complemented morphometric data...
Morphologically similar species are often overlooked but molecular techniques have been effective in signalling potential hidden diversity, boosting the documentation of unique evolutionary lineages and ecological diversity. Platynereis dumerilii massiliensis part a recognised complex, where differences reproductive biology mainly highlighted to date. Analyses DNA sequence data (COI, 16S rDNA D2 region 28S rDNA) populations apparent morphotype P. obtained from broader sampling area along...
The polychaete genus Laeonereis (Annelida, Nereididae) occurs over a broad geographic range and extends nearly across the entire Atlantic coast of America, from USA to Uruguay. Despite research efforts clarify its diversity systematics, mostly by morphological ecological evidence, there is still uncertainty, mainly concerning species culveri , which constitutes an old notorious case taxonomic ambiguity. Here, we revised molecular distribution based on multi-locus approach, including DNA...
Abstract Effective identification of species using short DNA fragments (DNA barcoding and metabarcoding) requires reliable sequence reference libraries known taxa. Both taxonomically comprehensive coverage content quality are important for sufficient accuracy. For aquatic ecosystems in Europe, barcode particularly if molecular tools to be implemented biomonitoring reports the context EU Water Framework Directive (WFD) Marine Strategy (MSFD). We analysed gaps two most databases, Barcode Life...
Abstract The accuracy of the identification unknown specimens using DNA barcoding and metabarcoding relies on reference libraries containing records with reliable taxonomy sequence quality. A rampant growth in barcode data led to a stringent need for curation, especially taxonomically difficult groups such as marine invertebrates. major effort curating deposited Barcode Life Data Systems (BOLD) has been undertaken during 8 th International Conference (Trondheim, Norway, 2019). For practical...
Abstract Eumida sanguinea is a recognized polychaete species complex which, in previous studies, has been reported to have additional undescribed diversity. We detected nine lineages by analysing DNA sequence data (mitochondrial: COI, 16S rRNA and nuclear loci: ITS region 28S rRNA) of E. morphotype populations from broader sampling effort European marine waters. Traditional morphological features failed provide consistent differences or unique characters that could be used distinguish these...
Recent studies reporting complexes of cryptic or pseudo-cryptic species with narrow geographic distributions have been challenging the cosmopolitan status a fair number marine benthic invertebrates. Morphologically similar are often overlooked but molecular techniques extremely effective in signalling potential hidden diversity which, complemented further detailed examination, might reveal unique morphological and ecological features. Evidence stasis, where no clear stable differences...
Abstract: Polychaetes are common in coastal and estuarine environments worldwide constitute one of the most complex groups marine invertebrates. The morpho-physiology female reproductive system (FRS) can be understood by using histological tools to describe cycle gametogenesis paths and, among other purposes, aiming identify differentiate polychaete species. However, this histology-based approach is rarely combined with molecular tools, which known accurately delimitate In same way,...
Abstract Despite the growing use and potential of DNA metabarcoding to improve expedite macrozoobenthos monitoring, its employment in Water Framework Directive (WFD) monitoring transitional ecosystems still remains largely unexplored pending proof-of-concept studies. In current study, we addressed this research gap by building upon biomonitoring network program Portuguese Environmental Agency (APA) benchmark against morphology-based approach for characterizing macrozoobenthic communities. We...