Molecular data have been suggesting the existence of a complex cryptic species within taxon Perinereis cultrifera , which has not fully explored yet. In this study, we performed morphological and molecular analysis (mtCOI-5P 16S rRNA 28SD2 ) specimens from intertidal marine brackish European localities, mostly focusing on Mediterranean Sea Canary Islands. Two major phylogenetic clades with at least 18 divergent (COI 19.8; 6.4-28.5%) completely sorted lineages were uncovered based original...

Abstract Biodiversity studies greatly benefit from molecular tools, such as DNA metabarcoding, which provides an effective identification tool in biomonitoring and conservation programmes. The accuracy of species‐level assignment, consequent taxonomic coverage, relies on comprehensive barcode reference libraries. role these libraries is to support species identification, but accidental errors the generation barcodes may compromise their accuracy. Here, we present R‐based application,...

DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy species-level assignment, consequent taxonomic coverage, relies on comprehensive barcode reference libraries, which, due to incompleteness, are currently recognized limitation for applications. In this study, we assembled library barcodes Atlantic Iberia marine species, assessed gaps in species coverage examined data ambiguities. Initially, an Iberian checklist the three dominant...

The introduction of non-indigenous species (NIS) is one the major threats to integrity European coastal ecosystems. DNA-based assessments have been increasingly adopted for monitoring NIS. However, accuracy taxonomic assignments largely dependent on completion and reliability DNA barcode reference libraries. As such, we aimed compile audit a library marine invertebrate NIS occurring in Europe. To do so, compiled list using three databases: Alien Species Information Network (EASIN), System...

Abstract The north‐east Atlantic (NEA) is an important and complex biogeographic region with a very rich marine fauna. However, little known about the role of Macaronesian islands in evolutionary history diversification invertebrates NEA. Among amphipods, members family Hyalidae are particularly common abundant intertidal rocky shores In this study, we aimed to investigate genetic structure diversity seven hyalid species inhabiting Macaronesian, European Moroccan coasts, focus on...

The accuracy of specimen identification through DNA barcoding and metabarcoding relies on reference libraries containing records with reliable taxonomy sequence quality. considerable growth in barcode data requires stringent curation, especially taxonomically difficult groups such as marine invertebrates. A major effort curating the Barcode Life Data Systems (BOLD) was undertaken during 8 th International Conference (Trondheim, Norway, 2019). Major taxonomic (crustaceans, echinoderms,...

Past molecular studies using mtDNA sequences and alloenzymes signalled the existence of at least two cryptic species within Hediste diversicolor morphotype, in European coasts. However, to this day, no new descriptions have been made. In study, we identified five completely sorted lineages a multi-locus approach, including mitochondrial DNA cytochrome oxidase I gene (COI-5P) nuclear markers ITS2 rRNA 28S rRNA. The data were complemented with morphometric measurements examined through...

Abstract The resistance of malaria parasites to available drugs continues grow, and this makes the need for new antimalarial therapies pressing. Aminoacyl‐tRNA synthetases (ARSs) are essential enzymes well‐established antibacterial targets so constitute a promising set development antimalarials. Despite their potential as drug targets, apicoplastic ARSs remain unexplored. We have characterized lysylation system Plasmodium falciparum , designed, synthesized, tested inhibitors based on...

DNA metabarcoding has the capacity to bolster current biodiversity assessment techniques, including early detection and monitoring of non-indigenous species (NIS). However, success this approach is greatly dependent on availability, taxonomic coverage reliability reference sequences in genetic databases, whose deficiencies can potentially compromise identifications at assignment step. In study we assessed lacunae availability sequence data from four barcodes (COI, 18S, rbcL matK) for NIS...

DNA metabarcoding has great potential to improve marine biomonitoring programs by providing a rapid and accurate assessment of species composition in zoobenthic communities. However, some methodological improvements are still required, especially regarding failed detections, primers efficiency incompleteness databases. Here we assessed the two different marker loci (COI 18S) three primer pairs detection through macrozoobenthic communities colonizing types artificial substrates (slate, PVC...

Abstract Aim DNA metabarcoding has great potential to improve biomonitoring in island's marine ecosystems, which are highly vulnerable global change and non‐indigenous species (NIS) introductions. However, the depth accuracy of taxonomic identifications mainly dependent on reference libraries containing representative reliable sequences for targeted species. In this study, we evaluated gaps availability their macroinvertebrates inhabiting Macaronesia's shallow habitats. Location Macaronesia...

Abstract Diversification and speciation of terrestrial organisms are anticipated in oceanic islands such as Macaronesia, a group Atlantic that have remained unconnected to continental landmasses. Hitherto, the diversification marine islands, especially those with low vagility, has received little direct empirical analysis using molecular markers. Here, we focus on case study, through applying multilocus approach investigate diversity evolution lacks planktonic larval stage, isopod genus...

Abstract We report on two new lineages of the Eumida sanguinea complex from Great Britain and describe one them as a species using multilocus approach, including mitochondrial DNA COI‐5P nuclear markers ITS (ITS1, 5.8S rRNA ITS2) 28S rRNA. The molecular analysis placed mackiei sp. nov. in monophyletic clade with 19.1% (COI), 10.1% (ITS) 1.7% (28S) mean distance to its nearest neighbour. Molecular diagnoses were also applied nine within E. complex. This was complemented morphometric data...

Morphologically similar species are often overlooked but molecular techniques have been effective in signalling potential hidden diversity, boosting the documentation of unique evolutionary lineages and ecological diversity. Platynereis dumerilii massiliensis part a recognised complex, where differences reproductive biology mainly highlighted to date. Analyses DNA sequence data (COI, 16S rDNA D2 region 28S rDNA) populations apparent morphotype P. obtained from broader sampling area along...

The polychaete genus Laeonereis (Annelida, Nereididae) occurs over a broad geographic range and extends nearly across the entire Atlantic coast of America, from USA to Uruguay. Despite research efforts clarify its diversity systematics, mostly by morphological ecological evidence, there is still uncertainty, mainly concerning species culveri , which constitutes an old notorious case taxonomic ambiguity. Here, we revised molecular distribution based on multi-locus approach, including DNA...

Sphaeromatid isopods, such as Dynamene, are common and abundant members of the invertebrate fauna littoral shallow sublittoral substrates. Six species Dynamene occur in northern hemisphere. Only two exist outside this range, Australia. The distribution various NE Atlantic-Black Sea axis has been controversial due to difficulty identification different species. This led inaccurate records their distribution, ultimately generating uncertain or faulty assessments on biodiversity these habitats....

eDNA metabarcoding has been increasingly employed in the monitoring of marine invertebrate non-indigenous species (NIS), particular using filtered seawater. However, comprehensive detection all NIS may require a diversity sampling substrates. To assess effectiveness 5 sample types (hard and artificial substrates, water, zooplankton) on recovery invertebrates' diversity, two marinas were monitored over three time points, COI 18S rRNA genes as DNA markers. We detected total 628 23 NIS, with...