A5013884525- Genetics, Aging, and Longevity in Model Organisms
- Kruppel-like factors research
- Mitochondrial Function and Pathology
- Alzheimer's disease research and treatments
- Cancer-related gene regulation
- Circadian rhythm and melatonin
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Lanthanide and Transition Metal Complexes
- RNA Research and Splicing
- Environmental Conservation and Management
- Metal complexes synthesis and properties
- Chronic Myeloid Leukemia Treatments
- Reproductive Biology and Fertility
- Genetic Syndromes and Imprinting
- Microbial Metabolic Engineering and Bioproduction
- Magnetism in coordination complexes
- Gut microbiota and health
- Genomics, phytochemicals, and oxidative stress
- Prion Diseases and Protein Misfolding
- Axon Guidance and Neuronal Signaling
- Signaling Pathways in Disease
- Hematopoietic Stem Cell Transplantation
Agency for Science, Technology and Research
2024
Genome Institute of Singapore
2024
Garvan Institute of Medical Research
2024
University of Oxford
2002-2023
The University of Sydney
2009-2020
Macquarie University
2014
Saint Louis University
1972
Abstract Interactions with bacteria play a major role in immune responses, ecology, and evolution of all animals, but they have been neglected until recently the case C. elegans. We report genetic investigation interaction elegans nematode-specific pathogen Microbacterium nematophilum, which colonizes rectum causes distinctive tail swelling its host. A total 121 mutants altered response to infection were isolated from selections or screens for bacterially unswollen (Bus) phenotype, using...
Transcription factors of the Sp/Klf (Krüppel-like factor) family regulate biological processes such as hematopoiesis, adipogenesis, and stem cell maintenance. Here we show that Bklf or Klf3 (Basic Krüppel-like represses Klf8 Factor 8) gene in vivo. Conversely, Eklf Klf1 (Erythroid activates gene. is driven by two promoters, both which contain multiple CACCC sites. can repress Klf1-mediated activation promoters. Chromatin immunoprecipitation experiments confirm occupies promoters...
TAU is a microtubule-associated protein that under pathological conditions such as Alzheimer's disease forms insoluble, filamentous aggregates. When twenty years after TAU's discovery the first transgenic mouse models were established, one declared goal was achieved modelling of authentic aggregate formation in form neurofibrillary tangles. However, we review here, it has become increasingly clear causes damage much before these aggregates develop. In fact, because scaffolding protein,...
Protein misfolding and aggregation as a consequence of impaired protein homeostasis (proteostasis) not only characterizes numerous age-related diseases but also the aging process itself. Functionally related to are, among others, ribosomal proteins, suggesting an intimate link between proteostasis aging. We determined by iTRAQ quantitative proteomic analysis in C. elegans how proteome changes with age response heat shock. Levels proteins mitochondrial chaperones were decreased aged animals,...
Understanding the genetic basis of gene expression can shed light on regulatory mechanisms underlying complex traits and diseases. Single-cell resolved measures RNA levels single-cell quantitative trait loci (sc-eQTLs) have revealed regulation that drives sub-tissue cell states types across diverse human tissues. Here, we describe first phase TenK10K, largest-to-date dataset matched whole-genome sequencing (WGS) RNA-sequencing (scRNA-seq). We leverage scRNA-seq data from over 5 million cells...
Protein with tau-like repeats (PTL-1) is the sole Caenorhabditis elegans homolog of tau and MAP2, which are members mammalian family microtubule-associated proteins (MAPs). In neurons, MAP2 segregated, being mainly localised to axon dendrite. particular, plays a crucial role in pathology, as elevated levels lead formation aggregates many neurodegenerative conditions including Alzheimer's disease. We used PTL-1 C. model biological functions protein without complication functional redundancy...
Countries around the world are attempting to navigate complex trade-offs between biodiversity and other land use objectives such as infrastructure expansion, with many adopting ‘net outcomes’ policies that aim ensure economic development leaves better off than before. The implementation of net outcomes often occurs on a project-by-project basis, which can lead missing opportunities for integrated thinking delivers across multiple objectives. Here, we present new practical framework...
Krüppel-like factor 3 (KLF3) is a transcriptional regulator that we have shown to be involved in the regulation of adipogenesis vitro. Here, report KLF3-null mice are lean and protected from diet-induced obesity glucose intolerance. On chow diet, plasma levels leptin decreased, adiponectin increased. Despite significant reductions body weight adiposity, wild-type knockout animals show equivalent energy intake, expenditure, excretion. To investigate molecular events underlying these...
The ability of DNA-binding transcription factors to recruit specific cofactors is central the mechanism by which they regulate gene expression. BKLF/KLF3, a member Krüppel-like factor family zinc finger proteins, potent transcriptional repressor that recruits CtBP co-repressor. We show here BKLF also four and half LIM domain protein FHL3. Different but closely linked regions mediate contact with CtBP2 present evidence interacts FHL3 demonstrate three proteins co-elute in gel filtration...
Krüppel-like factor 3 (KLF3/BKLF), a member of the (KLF) family transcription factors, is widely expressed transcriptional repressor with diverse biological roles. Although there considerable understanding molecular mechanisms that allow KLF3 to silence activity its target genes, less known about signal transduction pathways and post-translational modifications modulate in response physiological stimuli. We observed modified range different tissues found serine/threonine kinase...
Abstract Proteins of the Homeodomain-Interacting Protein Kinase (HIPK) family regulate an array processes in mammalian systems, such as DNA damage response, cellular proliferation and apoptosis. The nematode Caenorhabditis elegans has a single HIPK homologue called HPK-1. Previous studies have implicated HPK-1 longevity control suggested that this protein may be regulated stress-dependent manner. Here we set out to expand these observations by investigating role response heat oxidative...
Background: Tightly regulated pathways maintain the balance between proliferation and differentiation within stem cell populations. In Caenorhabditis elegans , germline is only tissue that maintained by stem‐like cells into adulthood. current study, we investigated role played a member of Homeodomain interacting protein kinase (HIPK) family serine/threonine kinases, HPK‐1, in development maintenance C. germline. Results: We report HPK‐1 required for promotion during Additionally, show soma...
PTL-1 is the sole homolog of MAP2/MAP4/tau family in Caenorhabditis elegans. Accumulation tau a pathological hallmark neurodegenerative diseases such as Alzheimer's disease. Therefore, reducing levels has been suggested therapeutic strategy. We previously showed that maintains age-related structural integrity neurons, implying excessive reduction tau-like protein detrimental. Here, we demonstrate regulation neuronal ageing by occurs via cell-autonomous mechanism. re-expressed null mutant...
Summary Oxidative stress is a central pathomechanism in Alzheimer's disease ( AD ) and other diseases with tau pathology. The Nrf2 transcription factor induces detoxification enzymes improves pathology cognition. Its homologue C. elegans SKN ‐1. We previously showed that the worm homologue, PTL ‐1, regulates neuronal aging lifespan. Here, we tested ‐1's involvement response. ptl‐1 mutant animals are hypersensitive to oxidative defective stress‐mediated nuclear accumulation of This defect can...
Regulation of chromatin is a key process in the developmental control gene expression. Many multi-subunit protein complexes have been found to regulate through modification histone residues. One such complex MOF acetyltransferase-containing NSL complex. While composition human and Drosophila has determined functions these investigated, existence an equivalent nematodes as Caenorhabditis elegans not yet explored. Here we summarise evidence, from our own work that others, homologues components...