A5061989120- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Protein Kinase Regulation and GTPase Signaling
- Receptor Mechanisms and Signaling
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular transport and secretion
- Phagocytosis and Immune Regulation
- Vaccine Coverage and Hesitancy
- Renal Diseases and Glomerulopathies
- CRISPR and Genetic Engineering
- Systemic Lupus Erythematosus Research
- Bacterial Infections and Vaccines
- Ion channel regulation and function
- Animal Virus Infections Studies
Monash University
2020-2025
Garvan Institute of Medical Research
2024-2025
Monash Medical Centre
2020-2022
Children's Medical Research Institute
2020
The University of Sydney
2020
Abstract Antigen-specific regulatory T cells (Tregs) suppress pathogenic autoreactivity and are potential therapeutic candidates for autoimmune diseases such as systemic lupus erythematosus (SLE). Lupus nephritis is associated with to the Smith (Sm) autoantigen human leucocyte antigen (HLA)-DR15 haplotype; hence, we investigated of Sm-specific Tregs (Sm-Tregs) disease. Here identify a HLA-DR15 restricted immunodominant Sm cell epitope using biophysical affinity binding assays, then...
Adeno-associated virus (AAV) vectors are quickly becoming the of choice for therapeutic gene delivery. To date, hundreds natural isolates and bioengineered variants have been reported. While factors such as high production titer low immunoreactivity important to consider, ability deliver genetic payload (physical transduction) drive transgene expression (functional remains most feature when selecting AAV clinical applications. Reporter assays commonly used methods determining vector fitness....
AAVs isolated from human liver are tropic for hepatocytes in vivo, but this phenotype becomes attenuated after passage culture.
Epidemiological studies and clinical trials suggest Bacillus Calmette-Guérin (BCG) vaccine has protective effects against coronavirus disease 2019 (COVID-19). There are now over 30 evaluating if BCG vaccination can prevent or reduce the severity of COVID-19. However, mechanism by which induce severe acute respiratory syndrome 2 (SARS-CoV-2)-specific T cell responses is unknown. Here, we identify 8 novel BCG-derived peptides with significant sequence homology to either SARS-CoV-2 NSP3...
Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed prostate, lung, cancers. However, little known about PSKH1 regulatory mechanisms, leading to its classification “dark” kinase. Here, we used biochemistry mass spectrometry define PSKH1’s consensus substrate motif, protein interactors, how including Ca 2+ sensor proteins, promote or suppress activity. Intriguingly, despite the absence of canonical Calmodulin binding...
Heterologous immunity, when the memory T cell response elicited by one pathogen recognizes another pathogen, has been offered as a contributing factor for high variability in coronavirus disease 2019 (COVID-19) severity outcomes. Here we demonstrate that sensitization with bacterial peptides can induce heterologous immunity to severe acute respiratory syndrome 2 (SARS-CoV-2) derived and vaccination SARS-CoV-2 spike protein peptides. Using silico prediction methods, identified 6 sequence...
Abstract Epidemiological studies suggest that the Bacillus Calmette-Guérin (BCG) vaccine may have protective effects against coronavirus disease 2019 (COVID-19); and, there are now more than 15 ongoing clinical trials seeking to determine if BCG vaccination can prevent or reduce severity of COVID-19 (1). However, mechanism by which induce a severe acute respiratory syndrome 2 (SARS-CoV-2) specific T cell response is unknown. Here, in silico , we identify 8 derived peptides with significant...
Abstract Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed prostate, lung cancers. However, little known about PSKH1 regulatory mechanisms, leading to its classification “dark” kinase. Here, we used biochemistry mass spectrometry define PSKH1’s consensus substrate motif, protein interactors, how including Ca 2+ sensor proteins, promote or suppress activity. Intriguingly, despite the absence of canonical Calmodulin binding...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a recently-discovered beta capable of infecting humans and causing Coronavirus Disease 2019 (COVID-19). COVID-19 causes variety pathologies that are still being understood. While vaccines specific for developed deployed throughout the world, another vaccine called Bacillus Calmette–Guérin (BCG) trialled to test whether it has protective effect against COVID-19. Epidemiological studies have identified negative...