A5073151789- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- S100 Proteins and Annexins
- Immune cells in cancer
- Pancreatic and Hepatic Oncology Research
- Ultrasound and Hyperthermia Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Neonatal Respiratory Health Research
- Photoacoustic and Ultrasonic Imaging
- Protein Kinase Regulation and GTPase Signaling
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cutaneous lymphoproliferative disorders research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Traumatic Brain Injury Research
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Cardiac Arrest and Resuscitation
- Dementia and Cognitive Impairment Research
- Platelet Disorders and Treatments
- Cancer Mechanisms and Therapy
- Ion channel regulation and function
- Ubiquitin and proteasome pathways
- Ultrasound Imaging and Elastography
Walter and Eliza Hall Institute of Medical Research
2022-2025
The University of Melbourne
2022-2025
Defence Science and Technology Group
2020
Abstract Antibodies targeting “immune checkpoints” have revolutionized cancer therapy by reactivating tumor-resident cytotoxic lymphocytes, primarily CD8+ T cells. Interest in analogous pathways other lymphocytes is growing. Natural killer (NK) cells are key to immunosurveillance eradicating metastases and driving solid tumor inflammation. NK-cell antitumor function dependent on the cytokine IL15. Ablation of IL15 signaling inhibitor CIS (Cish) enhances immunity increasing metabolism...
Abstract Epithelial-mesenchymal transition (EMT) is a continuum that includes epithelial, partial EMT, and mesenchymal states, each of which associated with cancer progression, invasive capabilities, ultimately, metastasis. We used lineage-traced sporadic model pancreatic to generate murine organoid biobank from primary secondary tumors, including sublines underwent EMT complete EMT. Using an unbiased proteomics approach, we found morphology predicts the state, solid organoids are signature....
Abstract A prevalent view in treating age-dependent disorders including Alzheimer’s disease (AD) is that the underlying amyloid plaque pathology must be targeted for cognitive improvements. In contrast, we report here repeated scanning ultrasound (SUS) treatment at 1 MHz frequency can ameliorate memory deficits APP23 mouse model of AD without reducing amyloid-β (Aβ) burden. Different from previous studies had shown Aβ clearance as a consequence blood-brain barrier (BBB) opening, here, BBB...
Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed prostate, lung, cancers. However, little known about PSKH1 regulatory mechanisms, leading to its classification “dark” kinase. Here, we used biochemistry mass spectrometry define PSKH1’s consensus substrate motif, protein interactors, how including Ca 2+ sensor proteins, promote or suppress activity. Intriguingly, despite the absence of canonical Calmodulin binding...
Cullin-5 (Cul5) coordinates assembly of cullin-RING-E3 ubiquitin (Ub) ligase (CRL) complexes that include Suppressor Cytokine Signaling (SOCS)-box-containing proteins. The SOCS-box proteins function to recruit specific substrates the complex for ubiquitination and degradation. In hematopoiesis, are best known regulating actions cytokines utilize JAK-STAT signaling pathway. However, roles most have not been studied in physiological contexts any Cul5/SOCS by several hematopoietic cytokines,...
There are a limited number of clinically useful serum biomarkers to predict tumor onset or treatment response in gastric cancer (GC). For this reason, we explored the proteome gp130Y757F murine model intestinal-type (IGC). We identified 30 proteins with significantly elevated expression early IGC and 12 that were late compared age- gender-matched wild-type mice. Within these signatures, there was an overlap 10 commonly both early- late-stage disease. These results highlight potential...
OBJECTIVE Posttraumatic headache (PTH) represents the most common acute and persistent symptom in children after concussion, yet there is no blood protein signature to stratify risk of PTH concussion facilitate early intervention. This discovery study aimed identify capillary markers, at emergency department (ED) presentation within 48 hours predict persisting 2 weeks postinjury. METHODS Capillary was collected using Mitra Clamshell device from aged 8–17 years who presented ED Royal...
Abstract Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed prostate, lung cancers. However, little known about PSKH1 regulatory mechanisms, leading to its classification “dark” kinase. Here, we used biochemistry mass spectrometry define PSKH1’s consensus substrate motif, protein interactors, how including Ca 2+ sensor proteins, promote or suppress activity. Intriguingly, despite the absence of canonical Calmodulin binding...
Abstract A prevalent view in treating age-dependent disorders including Alzheimer’s disease (AD) is that the underlying amyloid plaque pathology must be targeted for cognitive improvements. In contrast, we report here repeated scanning ultrasound (SUS) treatment at 1 MHz frequency can ameliorate memory deficits APP23 mouse model of AD without reducing amyloid-β (Aβ) burden. Different from previous studies had shown Aβ clearance as a consequence blood-brain barrier (BBB) opening, here, BBB...
Abstract Caspase-2, one of the most evolutionarily conserved member caspase family, is an important regulator cellular response to oxidative stress. Given that ferroptosis suppressed by antioxidant defense pathways, such as involving selenoenzyme glutathione peroxidase 4 (GPX4), we hypothesised caspase-2 may play a role in regulating ferroptosis. This study provides first demonstration and unprecedented function protecting cancer cells from undergoing ferroptotic cell death. Specifically,...
Supplementary Figure from TGFβ and CIS Inhibition Overcomes NK-cell Suppression to Restore Antitumor Immunity
Supplementary Figure from TGFβ and CIS Inhibition Overcomes NK-cell Suppression to Restore Antitumor Immunity
<div>Abstract<p>Antibodies targeting “immune checkpoints” have revolutionized cancer therapy by reactivating tumor-resident cytotoxic lymphocytes, primarily CD8<sup>+</sup> T cells. Interest in analogous pathways other lymphocytes is growing. Natural killer (NK) cells are key to immunosurveillance eradicating metastases and driving solid tumor inflammation. NK-cell antitumor function dependent on the cytokine IL15. Ablation of IL15 signaling inhibitor CIS...
<div>Abstract<p>Antibodies targeting “immune checkpoints” have revolutionized cancer therapy by reactivating tumor-resident cytotoxic lymphocytes, primarily CD8<sup>+</sup> T cells. Interest in analogous pathways other lymphocytes is growing. Natural killer (NK) cells are key to immunosurveillance eradicating metastases and driving solid tumor inflammation. NK-cell antitumor function dependent on the cytokine IL15. Ablation of IL15 signaling inhibitor CIS...
Abstract Skin inflammation is a complex process implicated in various dermatological disorders. The chronic proliferative dermatitis (cpd) phenotype driven by the cpd mutation (cpdm) Sharpin gene characterized dermal and epidermal abnormalities. Tumour necrosis factor (TNF) caspase-8-driven cell death causes pathogenesis of Sharpincpdm mice; however, role mind bomb 2 (MIB2), pro-survival E3 ubiquitin ligase involved TNF signaling, skin remains unknown. Here, we demonstrate that MIB2...
Here, samples of castor oil were extracted from Australian Ricinus communis seeds. The oils esterified and the derivatives characterised by gas chromatography-mass spectrometry (GC–MS). fatty acid composition was profiled, enabling trace components to be reported for first time. resulting data also subjected statistical analysis test applicability provenance geographic attribution purposes, but no discrimination found.
SUMMARY Epithelial-mesenchymal transition (EMT) is a continuum that includes epithelial, partial EMT (P-EMT) and mesenchymal states, each of which are associated with cancer progression, invasive capabilities ultimately metastasis. We have employed lineage traced sporadic model pancreatic to generate murine organoid biobank from primary secondary tumors, including sublines undergone P-EMT complete (C-EMT). Using an unbiased proteomics approach, we found the morphology organoids predicts...